In microbial leaching systems, the microbial tolerance cannot generally bear the environmental stress. Fluoride to a large extent could inhibit the growth and activity of the microbes,or even kill the microbes. The growth state and iron/sulfur oxidation activities of five representive bioleaching microorganisms ( A. ferrooxidans ATCC 23270, L. ferriphilumYSK,S. thermosulfidooxidans ST,A. thiooxidans A01 ,A. caldus S1 ) upon different fluoride stress were compared. It showed that the fluoride tolerance of A.ferrooxidans was the best, A. thioox...