Study of efflux pump gene expression in rifampicin-monoresistant Mycobacterium tuberculosis clinical isolates
作者：
Li, Guilian;Zhang, Jingrui;Guo, Qian;Wei, Jianhao;Jiang, Yi;...
期刊：
JOURNAL OF ANTIBIOTICS ,2015年68(7):431-435 ISSN：0021-8820
通讯作者：
Wan, Kanglin
作者机构：
[Zhao, Xiuqin; Zhao, Li-li; Zhang, Jingrui; Jiang, Yi; Liu, Zhiguang; Li, Guilian; Guo, Qian; Wan, Kanglin; Wei, Jianhao] Chinese Ctr Dis Control & Prevent, Natl Inst Communicable Dis Control & Prevent, TB Branch, State Key Lab Infect Dis Prevent & Control, Beijing 102206, Peoples R China.;[Zhao, Xiuqin; Zhao, Li-li; Zhang, Jingrui; Jiang, Yi; Liu, Zhiguang; Li, Guilian; Guo, Qian; Wan, Kanglin; Wei, Jianhao] Collaborat Innovat Ctr Diag & Treatment Infect Di, Hangzhou, Zhejiang, Peoples R China.;[Lu, Jianxin; Zhang, Jingrui; Wei, Jianhao] Wenzhou Med Coll, Key Lab Lab Med, Wenzhou, Zhejiang, Peoples R China.;[Zhang, Jingrui] Lab Gynecol & Obstetr Hosp, Shijiazhuang, Hebei, Peoples R China.;[Guo, Qian] South China Univ, Pathogen Biol Inst, Hengyang, Hunan, Peoples R China.
通讯机构：
[Wan, Kanglin] C;Chinese Ctr Dis Control & Prevent, Natl Inst Communicable Dis Control & Prevent, TB Branch, State Key Lab Infect Dis Prevent & Control, POB 5, Beijing 102206, Peoples R China.
摘要：
Rifampicin (RIF) resistance is a risk factor for poor outcome in tuberculosis (TB). In Mycobacterium tuberculosis, both target gene mutation and efflux pumps have major roles in the resistance to anti-TB drugs. This study aimed to determine whether RIF induces efflux pump activation in RIF-monoresistant M. tuberculosis strains. Here, we took advantage of 16 RIF-monoresistant M. tuberculosis clinical isolates to evaluate the expression of 27 putative drug efflux pump genes and measured the influence of four drug efflux pump inhibitors, carbonyl cyanide m-chlorophenyl hydrazone (CCCP), verapamil (VP), thioridazine (TZ) and chlorpromazine (CPZ), on the RIF MICs of these strains. Eight of the 16 RIF-monoresistant isolates carried mutations in rpoB and overexpressed one or two of the following putative efflux pump genes: Rv2333, drrB, drrC, Rv0842, bacA and efpA. CCCP, VP, TZ and CPZ lowered the RIF MICs greater than fourfold in 6, 12, 9 and 12 isolates, respectively. The lowered RIF MICs by VP and CPZ were identical and stronger than CCCP (P-values were all 0.033). In conclusion, the efflux pumps Rv2333, DrrB, DrrC, Rv0842, BacA and EfpA may have a role in RIF resistance in addition to classical mutations in the rpoB gene, and the addition of VP and CPZ could significantly increase RIF susceptibility in RIF-monoresistant M. tuberculosis.
语种：
英文
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从美国医疗卫生信息和管理系统协会的调查结果分析当前信息护士的发展状况
作者：
刘霖;陶红;刘珍;姜安丽
期刊：
中华医学图书情报杂志 ,2015年(4):9-14 ISSN：1671-3982
作者机构：
第二军医大学护理系人文护理学教研室,上海,200433;威斯康辛大学密尔沃基校区护理系 University of Wisconsin Milwaukee，美国威斯康辛州密尔沃基 53202;南华大学医学院临床技能教学中心,湖南衡阳,430400;[姜安丽; 刘霖] 第二军医大学;[刘珍] 南华大学
关键词：
护理信息学;信息护士;美国医疗卫生信息和管理系统协会
摘要：
分析了美国医疗卫生信息和管理系统协会（HIMSS）2004-2014年对美国信息护士工作现状的4次调查结果,从学历、薪酬、资格认证、教育水平、职业满意度、工作经验、主要工作职责等方面,总结出当前美国信息护士的几大特征：高学历且需交叉学科背景、收入丰厚职业满意度高、临床工作背景不可或缺、职业呈体系化发展趋势,为我国护理人员加深对信息护士的认识提供参考。
语种：
中文
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Low expression of spindle checkpoint protein, Cenp-E, causes numerical chromosomal abnormalities in HepG-2 human hepatoma cells
作者：
Liu, Bin;Luo, Hongmei;Wu, Guang;Liu, Jing;Pan, Jie;...
期刊：
ONCOLOGY LETTERS ,2015年10(5):2699-2704 ISSN：1792-1074
通讯作者：
Liu, Zhuoran
作者机构：
[Liu, Bin; Liu, Jing; Liu, Zhuoran; Wu, Guang; Pan, Jie] Univ South China, Affiliated Hosp 2, Dept Lab Med, Hengyang 4210001, Hunan, Peoples R China.;[Luo, Hongmei] Univ South China, Dept Histol & Embryol, Hengyang 4210001, Hunan, Peoples R China.;[Liu, Zhuoran] Univ South China, Affiliated Hosp 2, Dept Lab Med, 35 Jiefang Rd, Hengyang 4210001, Hunan, Peoples R China.
通讯机构：
[Liu, Zhuoran] U;Univ South China, Affiliated Hosp 2, Dept Lab Med, 35 Jiefang Rd, Hengyang 4210001, Hunan, Peoples R China.
关键词：
centromere-associated protein E;chromosome numerical abnormality;hepatoma;spindle checkpoint protein
摘要：
The aim of the present study was to investigate the expression, localization and role of centromere-associated protein E (Cenp-E) in hepatoma cells. The Cenp-E mRNA expression levels in the HepG-2 human hepatocellular carcinoma and LO2 normal hepatic cell lines following treatment with nocodazole were detected by reverse transcription-quantitative polymerase chain reaction (RT-qPCR). Furthermore, the localization and expression of Cenp-E protein in the two cell types was visualized using indirect immunofluorescence. RT-qPCR was also performed to detect the Cenp-E mRNA expression levels in LO2 cells before and after RNA interference. Additional evaluation of the function of interfered cells was performed using indirect immunofluorescence. The results of RT-qPCR demonstrated that the protein expression levels of Cenp-E in the two cell lines prior to treatment with nocodazole were not significantly different (P>0.05). However, the upregulation of Cenp-E expression levels in the LO2 cells was significantly higher compared with that in the HepG-2 cells during cell division (P<0.05). Indirect immunofluorescence analysis indicated that the Cenp-E protein was predominantly located in the nucleus, and that Cenp-E protein expression in nuclei with abnormal mitosis was markedly lower compared with that in nuclei exhibiting normal mitosis. Indirect immunofluorescence also determined that the ratio of dyskaryosis was significantly higher in cells that had undergone Cenp-E interference compared with normal cells. Thus, the present study indicated that the low expression of Cenp-E mRNA may be an important reason for numerical chromosomal abnormalities in human hepatoma cells. © 2015, Spandidos Publications. All rights reserved.
语种：
英文
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MiR-210 Links Hypoxia With Cell Proliferation Regulation in Human Laryngocarcinoma Cancer
作者：
Zuo, Jianhong;Wen, Meiling;Lei, Mingsheng;Peng, Xiang;Yang, Xuefeng* ;...
期刊：
JOURNAL OF CELLULAR BIOCHEMISTRY ,2015年116(6):1039-1049 ISSN：0730-2312
通讯作者：
Yang, Xuefeng
作者机构：
[Zuo, Jianhong; Liu, Zhigang] Shenzhen Univ, Sch Med, State Key Lab Resp Dis Allergy, Shenzhen 518060, Guangdong, Peoples R China.;[Zuo, Jianhong; Wen, Meiling; Yang, Xuefeng] Univ South China, Affiliated Nanhua Hosp, Hengyang 421001, Hunan, Peoples R China.;[Zuo, Jianhong] Univ South China, Sch Med, Hengyang 421001, Hunan, Peoples R China.;[Lei, Mingsheng] Zhangjiajie City Hosp, Dept Resp & Crit Care Med, Zhangjiajie 427000, Hunan, Peoples R China.;[Peng, Xiang] Shenzhen Univ, Sch Optoelect, Shenzhen 518060, Guangdong, Peoples R China.
通讯机构：
[Yang, Xuefeng] U;Univ South China, Affiliated Nanhua Hosp, Hengyang 421001, Hunan, Peoples R China.
关键词：
MiR-210;HYPOXIA;CELL PROLIFERATION;LARYNGOCARCINOMA CANCER;FGFRL1
摘要：
ABSTRACT The microRNA hsa‐miR‐210 (miR‐210) is associated with hypoxia; however its function has not fully identified. In the present study, we aim to detect its role concerning proliferation in Laryngocarcinoma. We found that miR‐210 was highly expressed in hypoxia, which inhibited proliferation by inducing cell cycle arrest in G1/G0 as well as apoptosis. We further identified that miR‐210 targeted fibroblast growth factor receptor‐like 1 (FGFRL1). Down regulation of FGFRL1 decreased cell proliferation by promoting proportion of cells in G1/G0 phase and decreasing in S and G2/M phases. Moreover, overexpression of FGFRL1 effectively released the miR‐210‐induced suppression of SCC10A cell proliferation. Expression of miR‐210 repressed tumor xenograft growth in vivo as well. Together, our findings reveal a new mechanism of adaptation to hypoxia that miR‐210 inhibits the proliferation via inducing cell cycle arrest and apoptosis by the targeting of FGFRL1. J. Cell. Biochem. 116: 1039–1049, 2015. © 2015 Wiley Periodicals, Inc.
语种：
英文
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Bom m 9 from Bombyx mori is a novel protein related to asthma
作者：
Zuo, Jianhong;Lei, Mingsheng;Yang, Rui;Liu, Zhigang*
期刊：
MICROBIOLOGY and IMMUNOLOGY ,2015年59(7):410-418 ISSN：0385-5600
通讯作者：
Liu, Zhigang
作者机构：
[Zuo, Jianhong; Liu, Zhigang; Yang, Rui] Shenzhen Univ, Sch Med, Shengzhen Univ, State Key Lab Resp Dis Allergy, Shenzhen 518060, Guangdong, Peoples R China.;[Zuo, Jianhong] Shenzhen Univ, Coll Optoelect Engn, Minist Educ & Guangdong Prov, Key Lab Optoelect Devices & Syst, Shenzhen 518060, Peoples R China.;[Zuo, Jianhong] Univ South China, Sch Med, Hengyang 421001, Hunan, Peoples R China.;[Lei, Mingsheng] Zhangjiajie City Hosp, Dept Resp & Crit Care Med, Zhangjiajie 427000, Hunan, Peoples R China.;[Lei, Mingsheng] Wuhan Univ, Zhongnan Hosp, Wuhan 430071, Hubei, Peoples R China.
通讯机构：
[Liu, Zhigang] S;Shenzhen Univ, Sch Med, Shengzhen Univ, State Key Lab Resp Dis Allergy, Shenzhen 518060, Guangdong, Peoples R China.
关键词：
asthma;Bom m 9;proteomics;silkworm (Bombys mori) pupa
摘要：
The silkworm (Bombyx mori) can cause severe IgE-mediated allergic disease, however, the mechanism remains unclear. The aim of this study was to investigate the immunologic mechanism by which silkworms induce allergy. Whole silkworm pupa proteins were separated by SDS-PAGE and 2-D PAGE. Then, IgE-binding proteins were detected by immunoblotting with sera of patients having an allergy to Bombyx mori. After tryptic digestion, the peptides of IgE-binding proteins were analyzed by matrix-assisted laser desorption ionization tandem time-of-flight mass spectrometry or tandem mass spectrometry. Database searches were used to identify allergens in silkworm pupa, after which Bom m 9 was to construct an asthma model. Thus, in the current study, a mouse asthma model was constructed with Bom m 9.
语种：
英文
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Multicenter Research on the BACTEC MGIT 960 system for the second-line drugs susceptibility testing of Mycobacterium tuberculosis in China
作者：
Zhao, Li-li;Liang, Ya-ping;Huang, Ming-xiang;Tan, Yun-hong;Jiang, Yuan;...
期刊：
Diagnostic Microbiology and Infectious Disease ,2013年77(4):330-334 ISSN：0732-8893
通讯作者：
Wan, Kang-lin
作者机构：
[Zhao, Li-li; Liu, Zhiguang; Jiang, Yi; Chen, Yan; Wan, Kang-lin] Chinese Ctr Dis Control & Prevent, State Key Lab Infect Dis Prevent & Control, Natl Reference Lab TB, Natl Inst Communicable Dis Control & Prevent, Beijing 102206, Peoples R China.;[Gao, Man; Liang, Ya-ping] Shaanxi TB Prevent & Treatment Hosp, Xian 710100, Peoples R China.;[Huang, Ming-xiang] Fujian Med Univ, Clin Teaching Hosp, Fuzhou Pulm Hosp, Fuzhou 350008, Peoples R China.;[Chen, Zhongnan; Tan, Yun-hong] Hunan Inst TB Control, Changsha 410013, Hunan, Peoples R China.;[Wu, Jie; Jiang, Yuan] Shanghai Municipal Ctr Dis Control & Prevent, Dept TB Prevent & Control, Shanghai 200336, Peoples R China.
通讯机构：
[Wan, Kang-lin] C;Chinese Ctr Dis Control & Prevent, State Key Lab Infect Dis Prevent & Control, Natl Reference Lab TB, Natl Inst Communicable Dis Control & Prevent, Beijing 102206, Peoples R China.
关键词：
BACTEC MGIT 960 system;Drug susceptibility testing;Lowenstein-Jensen proportion method;Multicenter research;Mycobacterium tuberculosis;The second-line drugs
摘要：
The reliability of the BACTEC MGIT 960 system for the second-line drugs (capreomycin [CPM], kanamycin [KAN], ofloxacin [OFX] and ethionamide [ETH]) susceptibility testing (DST) of Mycobacterium tuberculosis (M. tuberculosis) was compared to that of traditional Lowenstein-Jensen (L-J) proportion method (PM) among four different sites in China. After resolution of discrepant results by retesting the strains using both methods in the National Reference Laboratory of tuberculosis, the overall concordance values between the 2 systems were 99.7% (kappa value: 0.97) for CPM, 99.7% (kappa value: 0.97) for KAN, 100.0% (kappa value: 1.00) for OFX, and 98.6% (kappa value: 0.95) for ETH. The average turnaround time with BACTEC MGIT 960 system among four sites was 8.9 ± 1.7 days, significantly shorter than 28 days with the traditional L-J PM. Therefore, the BACTEC MGIT 960 system is a reliable and rapid method for the second-line drug susceptibility testing of tuberculosis in China. Notably, a stricter quality control program should be routinely carried out when clinical laboratories perform the second-line DST with BACTEC MGIT 960 system. © 2013.
语种：
英文
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高等医学院校全科医学生临床技能培养对策的探索与实践——以南华大学为例
作者：
向琼;王汉群;张新华;高天舒;杨琼;...
期刊：
医学教育研究与实践 ,2013年21(6):1095-1097+1156 ISSN：2096-3181
作者机构：
南华大学临床技能教学中心,湖南衡阳,421001;南华大学医学院,湖南衡阳,421001;[刘珍; 王汉群; 向琼; 文格波; 熊文昊; 杨琼; 高天舒; 黄靓; 张新华] 南华大学
关键词：
全科医学生;临床技能;培养对策
摘要：
加强高等医学院校全科医学生的临床技能培养,是培养高质量全科医生的重要途径和关键环节.在分析基层医生知识技能现状的基础上,提出高等医学院校创建全科医学生临床技能培养的方式方法,应当合理定位教学目标,优化整合教学内容,注重全科医学思维培养,关注人文素质培养,强化质量监控.
语种：
中文
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Dark rearing maintains tyrosine hydroxylase expression in retinal amacrine cells following optic nerve transection
作者：
Wan, Wei;Liu, Zhenghai;Wang, Xiaosheng;Luo, Xuegang*
期刊：
中国神经再生研究：英文版 ,2012年7(1):18-23 ISSN：1673-5374
通讯作者：
Luo, Xuegang
作者机构：
[Wan, Wei; Wang, Xiaosheng; Luo, Xuegang] Cent S Univ, Dept Human Anat & Neurobiol, Xiangya Med Coll, Changsha 410013, Hunan, Peoples R China.;[Wan, Wei; Liu, Zhenghai] Univ S China, Dept Human Anat, Hengyang 421001, Hunan, Peoples R China.
通讯机构：
[Luo, Xuegang] C;Cent S Univ, Dept Human Anat & Neurobiol, Xiangya Med Coll, Changsha 410013, Hunan, Peoples R China.
关键词：
optic nerve transection;tyrosine hydroxylase;dark rearing;amacrine cells;peripheral nerve injury;neural regeneration
摘要：
The present study examined changes in retinal tyrosine hydroxylase (TH) expression in rats having undergone optic nerve transection and housed under a normal day/night cycle or in the dark. The aim was to investigate the effects of amacrine cells on axonal regeneration in retinal ganglion cells and on the synapses that transmit visual signals. The results revealed that retinal TH expression gradually decreased following optic nerve transection in rats housed under a normal day/night cycle, reaching a minimum at 5 days. In contrast, retinal TH expression decreased to a minimum at 1 day following optic nerve transection in dark reared rats, gradually increasing afterward and reaching a normal level at 5-7 days. The number of TH-positive synaptic particles correlated with the TH levels, indicating that dark rearing can help maintain TH expression during the synaptic degeneration stage (5-7 days after optic nerve injury) in retinal amacrine cells.
语种：
英文
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Over-expression of eukaryotic translation initiation factor 4 gamma 1 correlates with tumor progression and poor prognosis in nasopharyngeal carcinoma
作者：
Tu, Luxia;Liu, Zhen;He, Xiufang;He, Ying;Yang, Huiling;...
期刊：
Molecular Cancer ,2010年9(1):1-11 ISSN：1476-4598
通讯作者：
Li, Xin
作者机构：
[Jiang, Qingping; Xiao, Guanghui; Tu, Luxia; Yao, Kaitai; Xie, Siming; Fang, Weiyi; Liu, Zhen; Li, Xin] So Med Univ, Minist Educ & Guangdong Prov, Key Lab Transcript & Prote Human Fatal Dis, Canc Res Inst, Guangzhou 510515, Guangdong, Peoples R China.;[He, Xiufang] Peoples Hosp Zhongshan City, Dept Otorhinolaryngol, Guangzhou 528403, Guangdong, Peoples R China.;[Tu, Luxia] So Med Univ, Nanfang Hosp, Dept Otorhinolaryngol, Guangzhou 510515, Guangdong, Peoples R China.;[He, Ying] Nanchang Univ, Affiliated Hosp 1, Dept Pathol, Nanchang 330006, Jiangxi, Peoples R China.;[Yang, Huiling] Univ S China, Affiliated Hosp 1, Inst Clin Med, Hengyang City 421001, Hunan, Peoples R China.
通讯机构：
[Li, Xin] S;So Med Univ, Minist Educ & Guangdong Prov, Key Lab Transcript & Prote Human Fatal Dis, Canc Res Inst, Guangzhou 510515, Guangdong, Peoples R China.
关键词：
Inflammatory Breast Cancer;Eukaryotic Translation Initiation Factor;PDCD4 Expression;Nasopharyngeal Tissue;Final Staining Score
摘要：
Background: The aim of the present study was to analyze the expression of eukaryotic translation initiation factor 4 gamma 1 (EIF4G1) in nasopharyngeal carcinoma (NPC) and its correlation with clinicopathologic features, including patients' survival time.Methods: Using real-time PCR, we detected the expression of EIF4G1 in normal nasopharyngeal tissues, immortalized nasopharyngeal epithelial cell lines NP69, NPC tissues and cell lines. EIF4G1 protein expression in NPC tissues was examined using immunohistochemistry. Survival analysis was performed using Kaplan-Meier method. The effect of EIF4G1 on cell invasion and tumorigenesis were investigated.Results: The expression levels of EIF4G1 mRNA were significantly greater in NPC tissues and cell lines than those in the normal nasopharyngeal tissues and NP69 cells (P < 0.001). Immunohistochemical analysis revealed that the expression of EIF4G1 protein was higher in NPC tissues than that in the nasopharyngeal tissues (P < 0.001). In addition, the levels of EIF4G1 protein in tumors were positively correlated with tumor T classification (P = 0.039), lymph node involvement (N classification, P = 0.008), and the clinical stages (P = 0.003) of NPC patients. Patients with higher EIF4G1 expression had shorter overall survival time (P = 0.019). Multivariate analysis showed that EIF4G1 expression was an independent prognostic indicator for the overall survival of NPC patients. Using shRNA to knock down the expression of EIF4G1 not only markedly inhibited cell cycle progression, proliferation, migration, invasion, and colony formation, but also dramatically suppressed in vivo xenograft tumor growth.Conclusion: Our data suggest that EIF4G1 can serve as a biomarker for the prognosis of NPC patients. © 2010 Tu et al; licensee BioMed Central Ltd.
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英文
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Oxidized low-density lipoprotein activates adipophilin through ERK1/2 signal pathway in RAW264.7 cells
作者：
Liu, Qingnan;Dai, Zhibing;Liu, Zhiqiang;Liu, Xiaohui;Tang, Chaoke;...
期刊：
生物化学与生物物理学报 ,2010年42(9):635-645 ISSN：1672-9145
通讯作者：
Yuan, Zhonghua
作者机构：
[Tang, Chaoke; Liu, Qingnan; Jiang, Zhisheng; Yi, Guanghui; Liu, Lushan; Liu, Zhiqiang; Wang, Zuo; Liu, Xiaohui; Yang, Yongzong; Yuan, Zhonghua] Univ S China, Key Lab Arteriosclerol Hunan Prov, Inst Cardiovasc Dis, Hengyang 421001, Peoples R China.;[Dai, Zhibing] Univ S China, Inst Pathobiol, Hengyang 421001, Peoples R China.;[Liu, Qingnan] YiYang Med Coll, Dept Basic Nursing, Yiyang 413000, Peoples R China.;[Dai, Zhibing] Cent Hosp Yiyang, Dept B Type Ultrason Diag, Yiyang 413000, Peoples R China.
通讯机构：
[Yuan, Zhonghua] U;Univ S China, Key Lab Arteriosclerol Hunan Prov, Inst Cardiovasc Dis, Hengyang 421001, Peoples R China.
关键词：
adipophilin;ERK1;atherosclerosis;PPAR gamma
摘要：
It has been reported that oxidized low-density lipoprotein (Ox-LDL) can increase the expression of adipophilin. However, the detailed mechanisms are not fully understood. The aim of this study was to investigate the mechanism of Ox-LDL on adipophilin expression and the intracellular lipid droplet accumulation. A mouse macrophage-like cell line, RAW264.7, was used throughout, and it was found that Ox-LDL induced adipophilin expression in a dose-dependent manner. Moreover, Ox-LDL induced peroxisome proliferator-activated receptor-γ (PPARγ) expression and PPARγ-specific inhibitor T0070907 abrogated Ox-LDL-induced adipophilin expression, but specific agonist GW1929 not. Furthermore, Ox-LDL induced phosphorylation of ERK1/2, and ERK1/2-specific inhibition by PD98059 suppressed the Ox-LDL-induced PPARγ and adipophilin expression. The results showed that ERK1/2 or PPARγ-specific inhibition decreased the amounts of intracellular lipid droplets. Meanwhile, the PPARγ-specific agonist increased intracellular lipid droplets. These results suggested that Ox-LDL-induced increase in adipophilin level via ERK1/2 activation is one of the mechanisms of inducing greater amounts of intracellular lipid droplets in RAW264.7 cells, which indicated that adipophilin is involved in atherosclerotic progression. © The Author 2010.
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英文
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青藤碱对吗啡依赖与戒断小鼠的小脑与脊髓中NO/nNOS系统的影响
作者：
刘珍;郑济芳;杨露青;易岚;胡弼
作者机构：
[刘珍; 郑济芳; 杨露青; 易岚; 胡弼] 南华大学生理学教研室;[刘珍; 郑济芳; 杨露青; 易岚; 胡弼] 南华大学遗传学教研室
会议名称：
湖南省生理科学会2006年度学术年会
会议时间：
2007-01-12
会议地点：
中国湖南长沙
摘要：
为探讨青藤碱对吗啡依赖与戒断小鼠的小脑、胸腰段脊髓中NO/nNOS系统的影响及作用机制,采用吗啡剂量递增法,建立小鼠吗啡依赖模型,纳洛酮催瘾,评价小鼠急性戒断时扭体、齿颤、直立、喷嚏、眼睑下垂等戒断症状,对成瘾小鼠用青藤碱治疗后,再用纳洛酮催瘾观察戒断症状。半定量RT-PCR测小脑和胸腰段脊髓nNOS的mRNA变化,化学比色法与硝酸还原酶法分别测小鼠小脑和胸腰段脊髓组织匀浆的nNOS酶的活性与NO
语种：
中文
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青藤碱对吗啡依赖与戒断小鼠小脑与脊髓NO/nNOS系统的影响
作者：
刘珍;郑济芳;杨露青;易岚;胡弼
期刊：
生理学报 ,2007年59(3):285-292 ISSN：0371-0874
通讯作者：
Liu, Z.
作者机构：
[刘珍; 郑济芳; 杨露青; 易岚; 胡弼] Department of Physiology, Nanhua University, Hengyang, 421001, China
关键词：
青藤碱;吗啡;戒断;一氧化氮合酶
摘要：
本文旨在探讨青藤碱对吗啡依赖与戒断小鼠的小脑和胸腰段脊髓中一氧化氮(nitric oxide,NO)/神经元型一氧化氮合酶(neural nitric oxide synthase,nNOS)系统的影响及作用机制,采用吗啡剂量递增法建立小鼠吗啡依赖模型,用纳洛酮激发戒断症状,评价小鼠急性戒断时齿颤、扭体、直立、喷嚏、眼睑下垂等戒断症状,对成瘾小鼠用青藤碱(40mg/kg,I.p.)治疗后,再用纳洛酮激发观察戒断症状.半定量RT-PCR检测小鼠小脑和胸腰段脊髓nNOS mRNA表达变化,化学比色法和硝酸还原酶法分别测定小鼠小脑和胸腰段脊髓组织匀浆的nNOS 活性与NO含量.结果显示:(1)青藤碱可以扭转由吗啡依赖引起的小鼠体重下降的趋势,减轻小鼠急性戒断时齿颤、扭体、直立、喷嚏、眼睑下垂等戒断症状;(2)青藤碱可降低小鼠吗啡依赖与戒断时在小脑与胸腰段脊髓中异常上调的nNOS mRNA水平,使酶活性下降到接近对照组水平.nNOS催化产生的NO含量与酶活性的变化一致;(3)单独使用青藤碱,小鼠没有出现类似吗啡引起的戒断症状,小脑与胸腰段脊髓中nNOS mRNA水平及酶活性没有异常升高.上述结果表明,青藤碱本身无成瘾性,但能显著减轻吗啡依赖小鼠的戒断征状,其作用机制可能与青藤碱影响小脑与脊髓中的NO/nNOS系统有关.
语种：
中文
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青藤碱对吗啡依赖与戒断小鼠脑组织nNOS活性的影响
作者：
刘珍;郑济芳;胡弼;刘运莲;周军媚
期刊：
中国应用生理学杂志 ,2007年23(3):297-299 ISSN：1000-6834
通讯作者：
Liu, Z.
作者机构：
[刘珍; 胡弼] 南华大学生理学教研室;[郑济芳; 刘运莲; 周军媚] 南华大学遗传学教研室
关键词：
青藤碱;吗啡;戒断;一氧化氮合酶
摘要：
目的:观察青藤碱对吗啡依赖与戒断小鼠中枢神经系统中大脑皮层、小脑、脑干的nNOS活性变化的影响.方法:剂量递增法建立小鼠吗啡依赖模型,青藤碱治疗前后用纳洛酮催瘾,观察戒断症状;化学比色法测各脑组织匀浆的nNOS活性.结果:青藤碱可扭转成瘾小鼠的体重下降趋势,减轻戒断症状;使由吗啡依赖与戒断引起异常变化的nNOS活性水平恢复到最终接近对照组水平.结论:青藤碱能显著减轻吗啡依赖小鼠的戒断征状,其机制可能与青藤碱影响各脑组织nNOS活性相关.
语种：
中文
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