Bom m 9 from Bombyx mori is a novel protein related to asthma

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成果类型：

期刊论文

作者：

Zuo, Jianhong;Lei, Mingsheng;Yang, Rui;Liu, Zhigang*

通讯作者：

Liu, Zhigang

作者机构：

[Zuo, Jianhong; Liu, Zhigang; Yang, Rui] Shenzhen Univ, Sch Med, Shengzhen Univ, State Key Lab Resp Dis Allergy, Shenzhen 518060, Guangdong, Peoples R China.

[Zuo, Jianhong] Shenzhen Univ, Coll Optoelect Engn, Minist Educ & Guangdong Prov, Key Lab Optoelect Devices & Syst, Shenzhen 518060, Peoples R China.

[Zuo, Jianhong] Univ South China, Sch Med, Hengyang 421001, Hunan, Peoples R China.

[Lei, Mingsheng] Zhangjiajie City Hosp, Dept Resp & Crit Care Med, Zhangjiajie 427000, Hunan, Peoples R China.

[Lei, Mingsheng] Wuhan Univ, Zhongnan Hosp, Wuhan 430071, Hubei, Peoples R China.

通讯机构：

[Liu, Zhigang] S

Shenzhen Univ, Sch Med, Shengzhen Univ, State Key Lab Resp Dis Allergy, Shenzhen 518060, Guangdong, Peoples R China.

语种：

英文

关键词：

asthma;Bom m 9;proteomics;silkworm (Bombys mori) pupa

期刊：

MICROBIOLOGY and IMMUNOLOGY

ISSN：

0385-5600

年：

2015

卷：

期：

页码：

410-418

DOI：

10.1111/1348-0421.12271

基金类别：

Major State Basic Research Development Program of China (973 Program)National Basic Research Program of China [2005CB121000]; National Nature Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81272960]; Technological Key Program of Guangdong province [2003A3080502]; Science and Technology Department of Hunan Province, China [2013WK2010]; Technological Plan of Shenzhen [200326]

机构署名：

本校为其他机构

院系归属：

医学院

摘要：

The silkworm (Bombyx mori) can cause severe IgE-mediated allergic disease, however, the mechanism remains unclear. The aim of this study was to investigate the immunologic mechanism by which silkworms induce allergy. Whole silkworm pupa proteins were separated by SDS-PAGE and 2-D PAGE. Then, IgE-binding proteins were detected by immunoblotting with sera of patients having an allergy to Bombyx mori. After tryptic digestion, the peptides of IgE-binding proteins were analyzed by matrix-assisted laser desorption ionization tandem time-of-flight mass spectrometry or tandem mass spectrometry. Databa...

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Bom m 9 from Bombyx mori is a novel protein related to asthma

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