作者：
Hu Rong;Li Guoqing;Xiao Zhefeng;Li Maoyu;Peng Fang;...
作者机构：
[Hu Rong; Li Guoqing; Xiao Zhefeng; Li Maoyu; Peng Fang; Shao Meiying; Chen Xiaojuan; Li Yuanyuan; Huang Ying; Zhan Xianquan; Chen Zhuchu] State Local Joint Engineering Laboratory for Anticancer Drugs,Hunan Engineering Laboratory for Structural Biology & Drug Design,Key Laboratory of Cancer Proteomics of Chinese Ministry of Health,Xiangya Hospital,Central South University,Changsha,P.R.China,410008;[Li Guoqing] Department of Biology,School of Pharmacy and Life Science,University of South China,Hengyang,Hunan,P.R.China,410008
会议名称：
第八届中国蛋白质组学大会
会议时间：
2013-9-7
会议地点：
重庆
会议主办单位：
中国生物化学与分子生物学会
会议论文集名称：
第八届中国蛋白质组学大会论文集
关键词：
human hepatocellular carcinoma cells;6-shogaol;endoplasmic reticulum stress;PERK;eIF2α;UPR;salubrinal
作者机构：
[Huang Ying; Li Guoqing; Xiao Zhefeng; Li Maoyu; Peng Fang; Hu Rong; Shao Meiying; Chen Xiaojuan; Li Yuanyuan; Zhan Xianquan; Chen Zhuchu] State Local Joint Engineering Laboratory for Anticancer Drugs,Hunan Engineering Laboratory for Structural BiologyDrug Design,Key Laboratory of Cancer Proteomics of Chinese Ministry of Health,Xiangya Hospital,Central South University,Changsha,P.R.China,410008;[Li Guoqing] Department of Biology,School of Pharmacy and Life Science,University of South China,Hengyang,Hunan,P.R.China,410008;[Li Yuanyuan] Medical College,Guangxi University of Science and Technology,Liuzhou,Guangxi,P.R.China,545005
作者机构：
[Xiao Zhefeng; Li Guoqing; Zhan Xianquan; Li Maoyu; Chen Xiaojuan; Peng Fang; Hu Rong; Shao Meiying; Huang Ying; Chen Zhuchu] State Local Joint Engineering Laboratory for Anticancer Drugs,Hunan Engineering Laboratory for Structural Biology & Drug Design,Key Laboratory of Cancer Proteomics of Chinese Ministry of Health,Xiangya Hospital,Central South University,Changsha,P.R.China,410008;[Li Guoqing] Department of Biology,School of Pharmacy and Life Science,University of South China,Hengyang,Hunan,P.R.China,410008
期刊：
BioMed Research International,2012年2012(3):510418-510429 ISSN：2314-6133
通讯作者：
Li Cui
作者机构：
[Li Cui; Chen Zhu-Chu; Xiao Zhi-Qiang; Xu Yan; Peng Fang; Duan Chao-Jun; Tang Can-E] Cent S Univ, Xiangya Hosp, Chinese Minist Hlth, Key Lab Canc Prote, Changsha 410008, Hunan, Peoples R China.;[Xu Yan] Judicial Police Gen Hosp, Changsha 410004, Hunan, Peoples R China.;[Cao Lan-Qin] Cent S Univ, Xiangya Hosp, Dept Gynecol & Obstet, Changsha 410008, Hunan, Peoples R China.;[Jin Long-Yu] Cent S Univ, Xiangya Hosp 3, Dept Cardiothorac Surg, Changsha 410013, Hunan, Peoples R China.;[Zeng Gu-Qing] Univ S China, Sch Med, Dept Gen Intro Surg, Hengyang 421001, Peoples R China.
通讯机构：
[Li Cui] C;Cent S Univ, Xiangya Hosp, Chinese Minist Hlth, Key Lab Canc Prote, Changsha 410008, Hunan, Peoples R China.
摘要：
Objective. To investigate the differential protein profile of human lung squamous carcinoma (HLSC) and normal bronchial epithelium (NBE) and provide preliminary results for further study to explore the carcinogenic mechanism of HLSC. Methods. Laser capture microdissection (LCM) was used to purify the target cells from 10 pairs of HLSC tissues and their matched NHBE, respectively. A stable-isotope labeled strategy using iTRAQ, followed by 2D-LC/Q-STAR mass spectrometry, was performed to separate and identify the differential expression proteins. Results. A total of 96 differential expression proteins in the LCM-purified HLSC and NBE were identified. Compared with NBE, 49 proteins were upregulated and 47 proteins were downregulated in HLSC. Furthermore, the expression levels of the differential proteins including HSPB1, CKB, SCCA1, S100A8, as well as S100A9 were confirmed by western blot and tissue microarray and were consistent with the results of quantitative proteomics. Conclusion. The different expression proteins in HLSC will provide scientific foundation for further study to explore the carcinogenic mechanism of HLSC.
摘要：
The development of cancer is a pathological process involving multiple environmental carcinogenic factors and genetic alterations. For decades, cancer researchers have focused on genomic and transcriptomic analyses. The completion of the Human Genome Project has opened the door to the post-genome era and oncoproteomics. Proteins play a critical role in tumorigenesis and influence the differences between normal cells and malignant cells. This report proposes the concept that cancer is a proteomic disease. This concept is based on examining protein expression profiles, post-translational modifications, and protein-protein interactions in carcinogenesis using recent advances in comparative, functional and structural proteomics. This approach provides a new way of viewing carcinogenesis, presents new clues in biomarker discovery for cancer diagnosis and therapy, and reveals important scientific findings and their significance to clinical applications.
期刊：
Journal of Histochemistry & Cytochemistry,2010年58(6):517-527 ISSN：0022-1554
通讯作者：
Chen, Zhuchu
作者机构：
[Li, Maoyu; Xiao, Zhiqiang; Li, Cui; Li, Feng; Peng, Fang; Xiao, Zhefeng; Chen, Zhuchu; Li, Guoqing] Cent S Univ, Xiangya Hosp, Key Lab Canc Prote, Chinese Minist Hlth, Changsha 410008, Hunan, Peoples R China.;[Yu, Yanhui; Li, Feng; Ouyang, Yongmei; Xiao, Zhefeng] Cent S Univ, Canc Res Inst, Xiangya Sch Med, Changsha 410008, Hunan, Peoples R China.;[Li, Guoqing] Univ S China, Sch Pharm & Life Sci, Dept Biol, Hengyang, Hunan, Peoples R China.;[Chen, Yongheng] Univ So Calif, Los Angeles, CA USA.;[Chen, Zhuchu] Cent S Univ, Xiangya Hosp, Key Lab Canc Prote, Chinese Minist Hlth, 87 Xiangya Rd, Changsha 410008, Hunan, Peoples R China.
通讯机构：
[Chen, Zhuchu] C;Cent S Univ, Xiangya Hosp, Key Lab Canc Prote, Chinese Minist Hlth, 87 Xiangya Rd, Changsha 410008, Hunan, Peoples R China.
关键词：
NPC;FFPE;iTRAQ;2D LC-MS/MS
摘要：
Formalin-fixed, paraffin-embedded (FFPE) tissue specimens represent a potentially valuable resource for protein biomarker investigations. In this study, proteins were extracted by a heat-induced antigen retrieval technique combined with a retrieval solution containing 2% SDS from FFPE tissues of normal nasopharyngeal epithelial tissues (NNET) and three histological types of nasopharyngeal carcinoma (NPC) with diverse differentiation degrees. Then two-dimensional liquid chromatography-tandem mass spectrometry coupled with isobaric tags for relative and absolute quantification (iTRAQ) labeling was employed to quantitatively identify the differentially expressed proteins among the types of NPC FFPE tissues. Our study resulted in the identification of 730 unique proteins, the distributions of subcellular localizations and molecular functions of which were similar to those of the proteomic database of human NPC and NNET that we had set up based on the frozen tissues. Additionally, the relative expression levels of cathepsin D, keratin8, SFN, and stathmin1 identified and quantified in this report were consistent with the immunohistochemistry results acquired in our previous study. In conclusion, we have developed an effective approach to identifying protein changes in FFPE NPC tissues utilizing iTRAQ technology in conjunction with an economical and easily accessible sample preparation method. (J Histochem Cytochem 58:517-527, 2010)
作者机构：
[Li Mao-Yu; Li Cui; Zhang Peng-Fei; Chen Zhu-Chu; Xiao Zhi-Qiang; Peng Fang; Li Guo-Qing; Li Mei-Xiang; Liu Ying-Fu] Cent S Univ, Key Lab Canc Prote, Chinese Minist Hlth, Xiangya Hosp, Changsha 410008, Hunan, Peoples R China.;[Li Feng; Li Mei-Xiang; Liu Ying-Fu] Cent S Univ, Canc Res Inst, Xiangya Sch Med, Changsha 410078, Hunan, Peoples R China.;[Li Mei-Xiang] Univ S China, Dept Histol & Embryol, Hengyang 421001, Peoples R China.;[Li Guo-Qing] Univ S China, Sch Life Sci & Technol, Hengyang 421001, Peoples R China.
通讯机构：
[Chen Zhu-Chu] C;Cent S Univ, Key Lab Canc Prote, Chinese Minist Hlth, Xiangya Hosp, Changsha 410008, Hunan, Peoples R China.
摘要：
AIM: To investigate the effects of macrophage migration inhibitory factor (MIF) on proliferation of human gastric cancer MGC-803 cells and expression of cyclin D1 and p27~(Kip1) in them, and further determine whether the effects are related to the PI3K/Akt signal transduction pathway. METHODS: Gastric cancer MGC-803 cells were cultured and then treated with 50 μg/L recombinant human MIF (rhMIF) with and without a PI3K inhibitor, LY294002 (25 μmol/L). MTT assay was used to detect the proliferation of MGC-803 cells. Cell cycle was detected by flow cytometry. Expression of cyclin D1 and p27~(Kip1) mRNA was by reverse transcription-polymerase chain reaction. Protein expression of phosphorylated Akt (p-Akt), Akt, cyclin D1 and p27~(Kip1) was examined by immunocytochemistry and Western blotting. RESULTS: rhMIF significantly stimulated the proliferation of MGC-803 cells and cell cycle progression from G1 phase to S phase in a concentration- and time-dependent manner. After the MGC-803 cells were treated with rhMIF for 24 h, the expression of cyclin D1 was significantly up-regulated compared with the cells not treated with rhMIF at both mRNA and protein levels (0.97± 0.02 vs 0.74±0.01,P = 0.002;0.98±0.05 vs 0.69±0.04,P =0.003). The p27~(Kip1) was downregulated but only statistically significant at the protein level. rhMIF significantly increased the expression of p-Akt, which reached the peak at 30 min, but did not affect the expression of Akt. However, LY294002 inhibited all the effects of rhMIF. CONCLUSION: Macrophage MIF increases the proliferation of gastric cancer cells, induces the expression of cyclin D1 at the transcriptional level and inhibits the expression of p27~(Kip1) at the post-transcriptional level via the PI3K/Akt pathway.
