期刊：
Journal of Orthopaedic Research,2013年31(10):1514-1519 ISSN：0736-0266
通讯作者：
Li, Kanghua
作者机构：
[Li, Kanghua; Dai, Zhu] Cent S Univ, Xiangya Hosp, Dept Orthoped, Changsha 410078, Hunan, Peoples R China.;[Chen, Zhiwei; Dai, Zhu; Liu, Chunlei; Yang, Lezhong; Liao, Ying] South China Univ, Affiliated Hosp 1, Dept Orthoped, Hengyang 421001, Hunan, Peoples R China.;[Ding, Wenjun] South China Univ, Affiliated Hosp 1, Clin Res Inst, Hengyang 421001, Hunan, Peoples R China.;[Li, Kanghua] Cent S Univ, Xiangya Hosp, Dept Orthoped, 87 Xiangya Rd, Changsha 410078, Hunan, Peoples R China.
通讯机构：
[Li, Kanghua] C;Cent S Univ, Xiangya Hosp, Dept Orthoped, 87 Xiangya Rd, Changsha 410078, Hunan, Peoples R China.
关键词：
in vitro;injury;integrative repair;meniscus;organ culture
摘要：
We investigated whether the implantation of juvenile allograft and minced meniscal fragments could improve the healing of avascular meniscal injuries, which cannot heal spontaneously. Concentric cylindrical explants were excised from the inner two-thirds of swine medial menisci. The inner cylinder consisted of a "sandwich" structure, with minced juvenile meniscal fragments, juvenile meniscal columns, minced mature meniscal fragments, or mature meniscal columns implanted in the middle. The explants were cultured in vitro for 2, 4, or 6 weeks. Interfacial meniscal repair was assessed by histology, immunohistochemistry, biomechanical testing, and confocal laser scanning microscopy. Histology and confocal microscopy results revealed that tissue repair and cell accumulation at the interface were best at all time points in the juvenile meniscal fragments group, followed by the juvenile columns, minced mature fragments, and mature columns groups, respectively. At 6 weeks, the implantation of juvenile allograft and minced meniscal fragments increased the shear strength, peak force, and energy to failure in the peripheral interface. Picosirius red/polarized light microscopy and immunohistochemistry results showed concurrent expression of type I and II collagen in the interfacial repair tissue. In conclusion, implantation of juvenile allograft and minced meniscal fragments could increase the healing of avascular meniscal injury in vitro.
作者机构：
[廖瑛; 范伟杰; 黄煊怀; 陈志伟] Department of Orthopedics, First Affiliated Hospital of University of South China, Hengyang 421001, Hunan Province, China;Huang Xuan-huai★, Master, Attending physician, Department of Orthopedics, First Affiliated Hospital of University of South China, Hengyang 421001, Hunan Province, China huangxuanhuai@163.com;As the epiphyseal plate has an important role in the growth and development of femur, the design of internal fixation should avoid the damage to the epiphyseal plate. Therefore, the positioning of pediatric femoral epiphyseal plate is particularly important.;To investigate the biomechanical property of the new femur neck screw internal fixation in treatment of children femoral neck fracture.;Twelve femurs were collected from six fresh children cadavers provided by Dissecting Room of University of South China. After exclusion of bone disease with X-ray film, three new children femoral neck screws and three Kirschner wires with the diameter of 2.0 mm were used for fixation. The biomechanical tests were conducted to detect the axial compression and torsion stiffness.
通讯机构：
[Liao, Y.] D;Department of Orthopedics, First Affiliated Hospital of University of South China, Hengyang 421001, Hunan Province, China
作者机构：
Department of Orthopedics, First Affiliated Hospital of the University of South China, Hengyang 421001, Hunan Province, China;Department of Orthopedics, South Branch of Yongzhou Central Hospital, Yongzhou 425006, Hunan Province, China;Dai Zhu★, Master, Attending physician, Department of Orthopedics, First Affiliated Hospital of the University of South China, Hengyang 421001, Hunan Province, China oliverdaizhu@sina.com.cn;Corresponding author: Yang Le-zhong, Master, Physician, Department of Orthopedics, South Branch of Yongzhou Central Hospital, Yongzhou 425006, Hunan Province, China toulangyingwang@163.com;Supported by: the Natural Science Foundation of Hunan Province, No.10JJ3094*
