摘要：
Leaching experiments were carried out using the Deino-ure, a genetically engineered bacterium of Deinococcus radiodurans, to investigate its impact on the migration patterns of uranium in soil. In comparison to Deino-wt and control group, the Deino-ure exhibited a decrease in leached uranium (VI) content, an increase in total soil uranium content, yielding a uranium retention rate of 60.98%. The equilibrium enrichment capacity was 53 mg/g, which was consistent with the pseudo-first-order reaction kinetic model (correlation coefficient R-2 = 0.99792). This validates the impact of Deino-ure on the forms of uranium in soil, demonstrating a transformation of the free state uranium into less migratory forms. The products of uranium enrichment were mainly the hydrated calcium uranyl oxide minerals.
摘要：
Deinococcus radiodurans (DR) exhibits strong resistance to ionizing radiation. In this study, by constructing a radiation-resistant genetically engineered strain overexpressing the Cs gene, the tolerance of the bacterium to aluminum ions was enhanced, thereby achieving the goal of microbial sustainable remediation of uranium-contaminated environments. Methods: 1. Extraction of the recombinant plasmid pRADK-Cs, transformation into DR, and verification. 2. Investigation of factors such as time and initial uranium concentration on the efficiency of uranium accumulation by the recombinant strain, characterized by changes in functional groups and surface morphology before and after accumulation using techniques such as scanning electron microscope. Conclusions: The recombinant strain Deino-Cs can reduce the inhibitory effect of aluminum ions on uranium accumulation capability, and it exhibits a higher uranium accumulation rate compared to the wild-type strain.
摘要：
Biosorption by microorganisms is an environmentally friendly and efficient method to adsorb heavy metals and radionuclieds. The purpose of this study was to transform urease gene (Ure) from Sporosarcina pasteurii DSM33 into D.radiodurans to prepare a recombinant Deino-Ure strains. The urease was used to decompose urea to produce CO32-, promoting calcium carbonate precipitation in the presence of Ca2+. This enhances the biomineralisation and U(IV) enrichment of D. radiodurans, which provides a scientific basis for the treatment of low enriched uranium contaminated soil. During Deino-Ure mineralization of U(VI), uranium may co-precipitate with calcium, forming a stable U(VI)/U (IV)-calcite mineral precipitate.
摘要：
A hydrothermal and ethanol activation method is proposed to synthesize Cu-BTC and graphite oxide composites (Cu-BTC/GO). The uranium dynamic adsorption behavior was investigated by varying the addition of graphene oxide. The result showed that Langmuir isotherm and quasi-secondary kinetic model (R-2 > 0.99) were more consistent with the adsorption process of uranium (VI) on this composite, and the maximum uranium adsorption was 150.50 mg g(-1). The thermodynamic parameters indicated that this adsorption process was spontaneous and endothermic. After five adsorption-desorption cycles, the adsorption efficiency can still reach 76.6-78.9%. This study will help to draw a promising roadmap to describe the adsorption performance of Cu-BTC/GO composites for uranium.
期刊：
Journal of Radioanalytical and Nuclear Chemistry,2022年331(2):655-664 ISSN：0236-5731
通讯作者：
He, Shuya;Xiao, Fangzhu
作者机构：
[He, Shuya; Xiao, Fangzhu; Chen, Luyao; Cheng, Conghui; Guo, Kexin; He, SY; Xiao, FZ; Li, Shanshan] Univ South China, Sch Publ Hlth, Hengyang 421001, Hunan, Peoples R China.;[Xie, Jingxi] Univ South China, Sch Chem & Chem Engn, Hengyang 421001, Hunan, Peoples R China.;[Xiao, Fangzhu] Univ South China, Hunan Engn Res Ctr Safety Control & Recycling Rad, Hengyang 421001, Hunan, Peoples R China.
通讯机构：
[He, SY; Xiao, FZ; Xiao, Fangzhu] U;Univ South China, Sch Publ Hlth, Hengyang 421001, Hunan, Peoples R China.;Univ South China, Hunan Engn Res Ctr Safety Control & Recycling Rad, Hengyang 421001, Hunan, Peoples R China.
关键词：
Deinococcus radiodurans;Heavy metal contamination;Radionuclide contamination;Biosorption
摘要：
This review thoroughly presents the recent progress of Deinococcus radiodurans, recombinant D. radiodurans and immobilized D. radiodurans as superior adsorbents to efficiently remove toxic heavy metals ions and radionuclides. Finally, a summary and prospect on the opportunities and challenges of D. radiodurans are provided.
摘要：
Deinococcus radiodurans (DR) is highly resistant to ionizing radiation. This study aims to convert dsrA gene into DR to construct radiation-resistant genetically engineered bacteria (Deino-dsrA) with high reducibility, so as to enhance the reducing and enrichment ability of DR. Methods: the recombinant vector pRADK-dsrA was extracted and transformed into DR. 1. Radiation resistant gene engineering bacteria containing dsrA gene were constructed successfully. 2. In the most favorable conditions,contributing to approximately 92.45% U (VI) was removed. 3. Autioxidant enzyme activities in Deino-dsrA is higher than in Deino-pRADK, excepting the content of malondialdehyde. The uranium enrichment ability of the Deino-dsrA is better than the wild DR, and dsrA gene can increase its antioxidation capability by increasing the activity of oxidase.
摘要：
A novel adsorbent magnetic functionalized m-carboxyphenyl azo calix[4]arene symmetrical sulfide derivative (Fe3O4/M-CCSSD) was fabricated by a series of simple reactions and characterized by FT-IR and SEM. The effects on U (VI) adsorption process were investigated at several conditions. The calculated data disclose that the adsorption process of U (VI) by Fe3O4/M-CCSSD is an endothermic spontaneous process, which well fits with the pseudo-second-order kinetic and Freundlich isotherm model. In the most favorable conditions, accounting for approximately 89% of U (VI) was removed. In addition, Fe3O4/M-CCSSD showed high selectivity for U (VI) and exceptional reusability.
期刊：
Journal of Radioanalytical and Nuclear Chemistry,2021年328(3):1265-1278 ISSN：0236-5731
通讯作者：
Fangzhu Xiao<&wdkj&>Guowen Peng
作者机构：
[Luo, Jiaqi; Peng, Guowen] Univ South China, Sch Chem & Chem Engn, Hengyang 421001, Hunan, Peoples R China.;[Xiao, Fangzhu; Zhu, Qiqi; Li, Shanshan] Univ South China, Sch Publ Hlth, Hengyang 421001, Hunan, Peoples R China.;[Chen, Fang; Peng, Guowen] Univ South China, Hunan Prov Engn Technol Res Ctr Uranium Tailings, Hengyang 421001, Hunan, Peoples R China.;[Xiao, Fangzhu; Peng, Guowen] Univ South China, Hunan Engn Res Ctr Safety Control & Recycling Rad, Hengyang 421001, Hunan, Peoples R China.
通讯机构：
[Fangzhu Xiao; Guowen Peng] S;School of Public Health, University of South China, Hengyang, People’s Republic of China<&wdkj&>Hunan Engineering Research Center for the Safety Control and Recycling of Radioactive Heavy Metal Pollutants, University of South China, Hengyang, People’s Republic of China<&wdkj&>School of Chemistry and Chemical Engineering, University of South China, Hengyang, People’s Republic of China<&wdkj&>Hunan Province Engineering Technology Research Center of Uranium Tailings Treatment, University of South China, Hengyang, People’s Republic of China<&wdkj&>Hunan Engineering Research Center for the Safety Control and Recycling of Radioactive Heavy Metal Pollutants, University of South China, Hengyang, People’s Republic of China
摘要：
In order to solve the problem of lack of resistance of active microorganisms to the complex ion environment in the treatment of uranium-containing wastewater, the Deino-flr-2 radiation-resistant genetically engineered bacteria containing flr-2 fluorine-resistant gene was constructed and characterized. Utilizing the reductase activity of Deinococcus radiodurans (DR) itself and the enrichment of U(VI) by the surface active functional groups of the bacteria, the treatment of simulated low-concentration uranium-containing wastewater will be realized. In the most favorable conditions, accounting for approximately 90% of U(VI) was removed. The calculated data disclosed that the enrichment process of U(VI) by Deino-flr-2 well fit with the pseudo-second-order kinetic and Freundlich isotherm model.
摘要：
Deinococcus radiodurans (DR) was used as a biological adsorbent for uranium adsorption of low-concentration uranium wastewater.The enrichment performance of the DR was investigated at several conditions,and characterized by SEM and EDS, genes with relatively different expression of DR enriched uranium were screened through RNA-seq. Through GO analysis and KEGG pathway enrichment analysis, to find the main regulatory pathways.The results show that: The DR under the culture condition, pH = 5, temperature 30 celcius, initial concentration of 10 mg/L, dosage of DR 16 mL, adsorption time for 60 min, uranium(VI) adsorption rate was 91%, the solution pH = 6.1 after enrichment; several genes were up-regulated or down-regulated, which involved in a variety of signaling pathways, mainly involving oxidative phosphorylation, various glucose metabolism and amino acid synthesis metabolism. Cytochrome C oxidase was significantly up-regulated.
摘要：
A novel material called m-carboxyphenyl azo calix[4]arene amine oxime derivatives (M-CCAOD) was synthesized by diazotization-coupling reaction and substitution reaction. Then the composite consisting of Fe3O4 and M-CCAOD was prepared through a facile self-assembly method. The as-synthesized Fe3O4/M-CCAOD composite was used to remove U(VI) from aqueous solutions under different conditions. The calculated data indicate that the adsorption process of uranium by Fe3O4/M-CCAOD is an exothermic spontaneous process, which well fits with the pseudo-second-order and Freundlich model. More significantly, the composite showed high selectivity for uranium and excellent reusability, demonstrating that Fe3O4/M-CCAOD may be a potential uranium adsorbent material.
