Research on the effect of Deinococcus radiodurans transformed by dsrA-flr-2 double gene on the enrichment performance of uranium(VI)

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作者信息关键词期刊信息基础信息归属信息摘要

成果类型：

期刊论文

作者：

Li, Shanshan;Xie, Jingxi;Luo, Jiaqi;Zhu, Qiqi;Shu, Yangzhen;...

通讯作者：

Xiao, Fangzhu;He, SY

作者机构：

[He, Shuya; Xiao, Fangzhu; Zhu, Qiqi; Xiao, FZ; Shu, Yangzhen; Li, Shanshan] Univ South China, Sch Publ Hlth, Hengyang 421001, Peoples R China.

[Xie, Jingxi; Luo, Jiaqi] Univ South China, Sch Chem & Chem Engn, Hengyang 421001, Peoples R China.

[Shu, Yangzhen; Peng, Guowen] Univ South China, Sch Resources Environm & Safety Engn, Hengyang 421001, Peoples R China.

通讯机构：

[Xiao, FZ; He, SY ] U

Univ South China, Sch Publ Hlth, Hengyang 421001, Peoples R China.

语种：

英文

关键词：

fluorine;uranium;uranyl nitrate;Article;bacterial gene;bacterial strain;bacterium identification;biomass;concentration (parameter);controlled study;Deinococcus radiodurans;dsrA flr 2 double gene;heavy metal removal;molecular cloning;nonhuman;pH;polymerase chain reaction;scanning electron microscopy;transgenic microorganism;transmission electron microscopy;waste component removal;waste water management

期刊：

Journal of Radioanalytical and Nuclear Chemistry

ISSN：

0236-5731

年：

2022

卷：

331

期：

页码：

2195-2207

DOI：

10.1007/s10967-022-08257-6

基金类别：

This work was supported by the Hunan Province Natural Science Foundation of China (Grant Numbers 2020JJ6050 and 2020JJ4077).

机构署名：

本校为第一且通讯机构

院系归属：

化学化工学院

公共卫生学院

摘要：

Strong-reducing gene (dsrA) and the fluorine-resistant gene (flr-2) were cloned into D. radiodurans to prepare a recombinant Deino-dsrA-flr-2 strains. Then, the influencing factors of uranium enrichment by Deino-dsrA-flr-2 and its enrichment mechanism were studied. When the initial concentration of uranium was 30mg/L, the pH was 5, the biomass was 0.016g and the enrichment time was 75min, the Deino-dsrA-flr-2 had the best remove effeciency of U(VI). SEM and TEM could clearly observe uranium deposits on the surface of bacteria; EDS also indicate...

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Research on the effect of Deinococcus radiodurans transformed by dsrA-flr-2 double gene on the enrichment performance of uranium(VI)

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