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Research on the effect of Deinococcus radiodurans transformed by dsrA-flr-2 double gene on the enrichment performance of uranium(VI)

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成果类型:
期刊论文
作者:
Li, Shanshan;Xie, Jingxi;Luo, Jiaqi;Zhu, Qiqi;Shu, Yangzhen;...
通讯作者:
Xiao, Fangzhu;He, SY
作者机构:
[He, Shuya; Xiao, Fangzhu; Zhu, Qiqi; Xiao, FZ; Shu, Yangzhen; Li, Shanshan] Univ South China, Sch Publ Hlth, Hengyang 421001, Peoples R China.
[Xie, Jingxi; Luo, Jiaqi] Univ South China, Sch Chem & Chem Engn, Hengyang 421001, Peoples R China.
[Shu, Yangzhen; Peng, Guowen] Univ South China, Sch Resources Environm & Safety Engn, Hengyang 421001, Peoples R China.
通讯机构:
[Xiao, FZ; He, SY ] U
Univ South China, Sch Publ Hlth, Hengyang 421001, Peoples R China.
语种:
英文
关键词:
Deinococcus radiodurans;Genetically engineered bacteria;Biological treatment;Bioreduction;Uranium-containing wastewater
期刊:
Journal of Radioanalytical and Nuclear Chemistry
ISSN:
0236-5731
年:
2022
卷:
331
期:
5
页码:
2195-2207
基金类别:
Hunan Province Natural Science Foundation of ChinaNatural Science Foundation of Hunan Province [2020JJ6050, 2020JJ4077]
机构署名:
本校为第一且通讯机构
院系归属:
化学化工学院
公共卫生学院
摘要:
Strong-reducing gene (dsrA) and the fluorine-resistant gene (flr-2) were cloned into D. radiodurans to prepare a recombinant Deino-dsrA-flr-2 strains. Then, the influencing factors of uranium enrichment by Deino-dsrA-flr-2 and its enrichment mechanism were studied. When the initial concentration of uranium was 30 mg/L, the pH was 5, the biomass was 0.016 g and the enrichment time was 75 min, the Deino-dsrA-flr-2 had the best remove effeciency of U(VI). SEM and TEM could clearly observe uranium deposits on the surface of bacteria; EDS also indic...

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