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Research on urease-modified Deinococcus radiodurans and the control mechanism of the contamination of U(VI)

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成果类型:
期刊论文
作者:
Liang, Yujin;Guo, Kexin;Jiang, Fan;Peng, Guowen;Xiao, Fangzhu
通讯作者:
Xiao, FZ;Peng, GW
作者机构:
[Xiao, Fangzhu; Xiao, FZ; Liang, Yujin; Guo, Kexin; Jiang, Fan] Univ South China, Sch Publ Hlth, Hengyang 421001, Peoples R China.
[Peng, Guowen] Univ South China, Sch Resources Environm & Safety Engn, Hengyang 421001, Peoples R China.
通讯机构:
[Xiao, FZ ; Peng, GW ] U
Univ South China, Sch Publ Hlth, Hengyang 421001, Peoples R China.
Univ South China, Sch Resources Environm & Safety Engn, Hengyang 421001, Peoples R China.
语种:
英文
关键词:
Deinococcus radiodurans;Uranium;Urease;Biomineralization;Microbially induced carbonate precipitation
期刊:
Journal of Radioanalytical and Nuclear Chemistry
ISSN:
0236-5731
年:
2024
页码:
1-16
基金类别:
Environmental Protection Research Project of Hunan Province; Independent Research Project of the China Academy of Radiation Protection (2022 Platform Project of the National Key Laboratory of Radiation Environment and Health for Environmental Protection -4); National Natural Science Foundation of China [42377076]; Natural Science Foundation of Hunan Province [2023JJ50129, 202002042158]; Hunan Provincial Innovation Foundation for Postgraduates [QL20230242]; [HBKYXM-2023021]
机构署名:
本校为第一且通讯机构
院系归属:
公共卫生学院
摘要:
Biosorption by microorganisms is an environmentally friendly and efficient method to adsorb heavy metals and radionuclieds. The purpose of this study was to transform urease gene (Ure) from Sporosarcina pasteurii DSM33 into D.radiodurans to prepare a recombinant Deino-Ure strains. The urease was used to decompose urea to produce CO32-, promoting calcium carbonate precipitation in the presence of Ca2+. This enhances the biomineralisation and U(IV) enrichment of D. radiodurans, which provides a scientific basis for the treatment of low enriched uranium contaminated soil. During Deino-Ure mineral...

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