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The reduction effect and mechanism of Deinococcus radiodurans transformed dsrA gene to uranyl ions

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成果类型:
期刊论文
作者:
Cheng, Conghui;Xie, Jingxi;Zhu, Qiqi;Chen, Luyao;Guo, Kexin;...
通讯作者:
He, Shuya;Xiao, Fangzhu
作者机构:
[He, Shuya; Xiao, Fangzhu; Chen, Luyao; Cheng, Conghui; Zhu, Qiqi; Guo, Kexin; He, SY; Xiao, FZ; Li, Shanshan] Univ South China, Sch Publ Hlth, Hengyang 421001, Hunan, Peoples R China.
[Xie, Jingxi] Univ South China, Sch Chem & Chem Engn, Hengyang 421001, Hunan, Peoples R China.
通讯机构:
[He, SY; Xiao, FZ] U
Univ South China, Sch Publ Hlth, Hengyang 421001, Hunan, Peoples R China.
语种:
英文
关键词:
Deinococcus radiodurans;Reduction gene dsrA;Uranium;Reduction enrichment;Oxidative stress
期刊:
Journal of Radioanalytical and Nuclear Chemistry
ISSN:
0236-5731
年:
2021
卷:
330
期:
3
页码:
1075-1090
基金类别:
Nature Science Foundation of Hunan ProvinceNatural Science Foundation of Hunan Province [2020JJ6050.2020JJ4077]
机构署名:
本校为第一且通讯机构
院系归属:
化学化工学院
公共卫生学院
摘要:
Deinococcus radiodurans (DR) is highly resistant to ionizing radiation. This study aims to convert dsrA gene into DR to construct radiation-resistant genetically engineered bacteria (Deino-dsrA) with high reducibility, so as to enhance the reducing and enrichment ability of DR. Methods: the recombinant vector pRADK-dsrA was extracted and transformed into DR. 1. Radiation resistant gene engineering bacteria containing dsrA gene were constructed successfully. 2. In the most favorable conditions,contributing to approximately 92.45% U (VI) was removed. 3. Autioxidant enzyme activities in Deino-dsr...

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