期刊：
FRONTIERS IN IMMUNOLOGY,2024年15:1289644 ISSN：1664-3224
通讯作者：
Li, ZY
作者机构：
[Li, Zhongyu; Fang, Chunxia; Li, ZY; Wang, Xinglv; Wu, Hongrong] Univ South China, Inst Pathogen Biol, Hengyang Med Coll, Sch Nursing,Hunan Prov Key Lab Special Pathogens P, Hengyang, Peoples R China.
通讯机构：
[Li, ZY ] U;Univ South China, Inst Pathogen Biol, Hengyang Med Coll, Sch Nursing,Hunan Prov Key Lab Special Pathogens P, Hengyang, Peoples R China.
关键词：
Chlamydia trachomatis;immune evasion;innate immune cells;innate immunity;survival and growth
摘要：
Chlamydia trachomatis, is a kind of obligate intracellular pathogen. The removal of C. trachomatis relies primarily on specific cellular immunity. It is currently considered that CD4(+) Th1 cytokine responses are the major protective immunity against C. trachomatis infection and reinfection rather than CD8(+) T cells. The non-specific immunity (innate immunity) also plays an important role in the infection process. To survive inside the cells, the first process that C. trachomatis faces is the innate immune response. As the "sentry" of the body, mast cells attempt to engulf and remove C. trachomatis. Dendritic cells present antigen of C. trachomatis to the "commanders" (T cells) through MHC-I and MHC-II. IFN-γ produced by activated T cells and natural killer cells (NK) further activates macrophages. They form the body's "combat troops" and produce immunity against C. trachomatis in the tissues and blood. In addition, the role of eosinophils, basophils, innate lymphoid cells (ILCs), natural killer T (NKT) cells, γδT cells and B-1 cells should not be underestimated in the infection of C. trachomatis. The protective role of innate immunity is insufficient, and sexually transmitted diseases (STDs) caused by C. trachomatis infections tend to be insidious and recalcitrant. As a consequence, C. trachomatis has developed a unique evasion mechanism that triggers inflammatory immunopathology and acts as a bridge to protective to pathological adaptive immunity. This review focuses on the recent advances in how C. trachomatis evades various innate immune cells, which contributes to vaccine development and our understanding of the pathophysiologic consequences of C. trachomatis infection.
期刊：
Journal of the American College of Cardiology,2015年66(16, Supplement):C122-C123 ISSN：0735-1097
作者机构：
[Liu, Feng; Hu, Hongjuan; Wang, Jiajie] Univ South China, Sch Nursing, Hengyang, Peoples R China.;[Kuang, Zemin] Capital Med Univ, Beijing Anzhen Hosp, Beijing, Peoples R China.
会议名称：
26th Great Wall International Congress of Cardiology (GW-ICC) / Asia Pacific Heart Congress (APHC) / International Congress of Cardiovascular Prevention and Rehabilitation (ICCPR)
作者机构：
[Chen, Xin] Hunan Tradit Chinese Med Coll, Zhuzhou, Peoples R China.;[Huang, Lei] Hunan Prov Hosp Tradit Chinese Med, Zhuzhou, Peoples R China.;[Ouyang, Xinping; He, Pingping] Univ South China, Inst Cardiovascular Res, Dept Physiol, Coll Med, Hengyang, Peoples R China.;[He, Pingping] Univ South China, Sch Nursing, Hengyang, Peoples R China.;[Wang, Bo; Liao, Huiying] Univ South China, First Affiliated Hosp, Hengyang, Peoples R China.
会议名称：
International Conference on Biological Engineering and Biomedical (BEAB)
会议时间：
JAN 10-12, 2014
会议地点：
Yichang, PEOPLES R CHINA
会议主办单位：
[Huang, Lei] Hunan Prov Hosp Tradit Chinese Med, Zhuzhou, Peoples R China.^[Chen, Xin] Hunan Tradit Chinese Med Coll, Zhuzhou, Peoples R China.^[Ouyang, Xinping;He, Pingping] Univ South China, Inst Cardiovascular Res, Dept Physiol, Coll Med, Hengyang, Peoples R China.^[He, Pingping] Univ South China, Sch Nursing, Hengyang, Peoples R China.^[Wang, Bo;Liao, Huiying] Univ South China, First Affiliated Hosp, Hengyang, Peoples R China.^[Lu, Suqing] Univ Guilin Med, Affiliated Hosp, Dept Urol, Guilin, Peoples R China.^[Su, Qi] Univ South China, Dept Pathol, Hengyang, Peoples R China.
摘要：
In this study, we down-regulate the expression of calreticulin (CRT) in differentiation of human leukemic HL-60 cells induced by diallyl disulfide (DADS) so as to find out the molecular mechanism of differentiation in HL-60 cells induced by DADS. By using several methods like Giemsa's staining, siRNA, MTT, qPCR, Westenbloting, flow cytometry etc., we tested the morphology changes of HL-60 cells, detected the expression of CRT before and after the use of siRNA and DADS, also we measured the proliferation of HL-60 cells. The results showed that DADS can apparently change the morphology of HL-60, furthermore both DADS and siRNA can decrease the expression of CRT. Once CRT was silenced, the proliferation of HL-60 was inhibited in the meantime the expression of CD33 and CD11b was significantly decreased. Therefore, DADS can down-regulates CRT-induced HL-60 differentiation meanwhile the silent CRT can induce the differentiation of HL-60 cells and enhance DADS-induced HL-60 cells differentiation.
作者机构：
[Ouyang, Xinping; Li, Jin-Feng; He, Pingping; Li, Yuanhui] Univ South China, Inst Cardiovasc Dis, Coll Med, Dept Physiol, Hengyang, Peoples R China.;[Wang, Bo; Liao, Huiying; Fu, Wan] Univ South China, First Affiliated Hosp, Hengyang, Peoples R China.;[Zhang, Tao] Univ South China, Second Affiliated Hosp, Hengyang, Peoples R China.;[He, Pingping] Univ South China, Sch Nursing, Hengyang, Peoples R China.
会议名称：
International Conference on Biological Engineering and Biomedical (BEAB)
会议时间：
JAN 10-12, 2014
会议地点：
Yichang, PEOPLES R CHINA
会议主办单位：
[Ouyang, Xinping;Li, Jin-Feng;Li, Yuanhui;He, Pingping] Univ South China, Inst Cardiovasc Dis, Coll Med, Dept Physiol, Hengyang, Peoples R China.^[Wang, Bo;Liao, Huiying;Fu, Wan] Univ South China, First Affiliated Hosp, Hengyang, Peoples R China.^[Zhang, Tao] Univ South China, Second Affiliated Hosp, Hengyang, Peoples R China.^[He, Pingping] Univ South China, Sch Nursing, Hengyang, Peoples R China.
摘要：
Objective: To test the effects of capsaicin on learning and memory ability in Alzheimer's disease (AD) mice. Results: Capsaicin (CAP) has no effect on spontaneous activity in APP/PS1 transgenic mice. CAP reduces the average escape latency and average swimming distance of Morris water maze in APP/PS1 transgenic mice. CAP adds academic record of Y maze in APP/PS1 transgenic mice. Conclusion: Capsaicin improves the function of learning and memory in AD mice.
作者机构：
[Liu, Jun; Zhang, Tao] Univ South China, Affiliated Hosp 2, Dept Urol, Hengyang, Peoples R China.;[He, Pingping] Univ South China, Inst Cardiovasc Dis, Coll Med, Dept Physiol, Hengyang, Peoples R China.;[Chen, Xin; Li, E.] Hunan Tradit Chinese Med Coll, Zhuzhou, Peoples R China.;[Peng, Zhe; Wang, Bo; Liao, Huiying; Fu, Wan] Univ South China, First Affiliated Hosp, Hengyang, Peoples R China.;[He, Pingping] Univ S China, Sch Nursing, Hengyang, Peoples R China.
会议名称：
International Conference on Biological Engineering and Biomedical (BEAB)
会议时间：
JAN 10-12, 2014
会议地点：
Yichang, PEOPLES R CHINA
会议主办单位：
[He, Pingping] Univ South China, Inst Cardiovasc Dis, Coll Med, Dept Physiol, Hengyang, Peoples R China.^[Zhang, Tao;Liu, Jun] Univ South China, Affiliated Hosp 2, Dept Urol, Hengyang, Peoples R China.^[Chen, Xin;Li, E.] Hunan Tradit Chinese Med Coll, Zhuzhou, Peoples R China.^[Wang, Bo;Liao, Huiying;Fu, Wan;Peng, Zhe] Univ South China, First Affiliated Hosp, Hengyang, Peoples R China.^[He, Pingping] Univ S China, Sch Nursing, Hengyang, Peoples R China.
摘要：
Object: To observe the influence of silencing the expression of YB-1 gene by RNA interference cell on proliferation and migration of prostate cancer. Methods: The YB-1 siRNA was transfected into PC-3 cells at logarithmic growth by liposome, expression levels of YB-1 mRNA and protein were detected by reverse transcription-polymerase chain reaction (RT-PCR) and Wesretn-blot, proliferation and invasion of PC-3 cell was detected by MTT assay and Transwell experiments. Results: After transfection for 48 h, compared with PC-3 cells non-transfected with plasmid, YB-1 mRNA levels and YB-1 protein levels in PC-3 cells transfected with recombinant plasmid pGenesil-1-YB-1-1 and recombinant plasmid pGenesil-1-YB-1-2 were significantly decreased (P< 0.05). YB-1 mRNA inhibition rates in PC-3 cells transfected with recombinant plasmid pGenesil-1-YB-1-1 and recombinant plasmid pGenesil-1-YB-1-2 were 36.23% and 39.42%, while YB-1 protein inhibition rates were 41.56% and55.33%. MTT results showed that the proliferation of PC-3 cells transfected with recombinant plasmid pGenesil-1YB- 1-1 and recombinant plasmid pGenesil-1-YB-1-2 was significantly decreased when compared with PC-3 cells non-transfected with plasmid (P< 0.05). Transwell migration experiments showed that the number of cells go through the membrane in PC-3 cells non-transfected, PC-3 cells transfected with pGenesil-1-YB-NC, PC-3 cells transfected with recombinant plasmid pGenesil-1-YB-1-1 and PC-3 cells transfected with recombinant plasmid pGenesil-1-YB-1-2, were(123.71+/-13.25),(135.73+/-14.18),(67.73+/-8.82) and (51.73+/-6.75) respectively (P<0.05). The migration of PC-3 cells was reduced by downregulating the expression of YB-1, also the invasiveness of PC-3 cells vitro was inhibited. Conclusions: cell proliferation and migration of prostate cancer was inhibited by RNA interference silencing the expression of YB-1 gene.
