期刊：
Journal of Radioanalytical and Nuclear Chemistry,2022年331(9):4047-4054 ISSN：0236-5731
通讯作者：
Pingping Huang
作者机构：
[Zeng, Weihao; Shen, Hao; Zhao, Tianji; Huang, Pingping; Sun, Junjie; Wu, Haoyang; Zhang, Jie; Wen, Xinhai] Univ South China, Sch Resource Environm & Safety Engn, Hengyang 421001, Hunan, Peoples R China.;[Huang, Pingping] Hunan Prov Engn Technol Res Ctr Uranium Tailings, Hengyang 421001, Hunan, Peoples R China.;[Hu, Zhitao] Univ South China, Coll Mech Engn, Hengyang 421001, Hunan, Peoples R China.
通讯机构：
[Pingping Huang] S;School of Resource Environment and Safety Engineering, University of South China, Hengyang, China<&wdkj&>Hunan Province Engineering Technology Research Center of Uranium Tailings Treatment, Hengyang, China
作者机构：
[Yang, H.; Mi, J.; Zeng, L.; Huang, Z.; Yang, N.; Zhang, Y.] Cent South Univ, Dept Med Oncol, Hunan Canc Hosp,Affiliated Canc Hosp, Lung Canc & Gastrointestinal Unit,Xiangya Sch Med, Changsha 410013, Peoples R China.;[Mi, J.; Huang, Z.; Yang, N.; Zhang, Y.] Univ South China, Grad Sch, Hengyang, Hunan, Peoples R China.;[Zhang, R.; Dong, X.; Tong, F.] Huazhong Univ Sci & Technol, Union Hosp, Tongji Med Coll, Canc Ctr, Wuhan 430022, Peoples R China.;[Xu, Q.] Qinghai Prov Peoples Hosp, Dept Med Oncol, Xining, Peoples R China.;[Lizaso, A.] Burning Rock Biotech, Guangzhou, Peoples R China.
通讯机构：
[X. Dong] C;[N. Yang; Y. Zhang] D;Cancer Center, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China<&wdkj&>Department of Medical Oncology, Lung Cancer and Gastrointestinal Unit, Hunan Cancer Hospital/The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha, China<&wdkj&>Graduate School, University of South China, Hengyang, Hunan, China
关键词：
EGFR mutation;de novo MET overexpression/amplification;EGFR-TKI;crizotinib;non-small-cell lung cancer
摘要：
In order to assess the feasibility of uranium removal technology from radioactive contaminated soil by combined bioleaching bacterial consortia, mixed bacterial culture and pure culture of four strains (Acidithiobacillus ferrooxidans ATCC 23,270, Leptosirillum ferripHilum YSK, Acidithiobacills thiooxidans A01, Acidithiobacillus ferrivorans YL15) were comparatively investigated on uranium removal from a radioactive contaminated soil. The results showed that the mixed bioleaching bacterial consortium exerted good adaptability to the surroundings, showing high biological activity and uranium removal capacity. In the whole cleaning process of uranium contaminated soil, the mixed bioleaching bacterial consortium showed complementary advantages, and the synergistic effect improved the uranium removal efficiency, and the maximum uranium removal of the combined bacteria was achieved to 85.81%. Additionally, the linear fitting analysis showed that the uranium removal was positively related to redox potential and negatively related to pH. Lastly, the soil residue after cleaning was detected by SEM/EDS. The results showed that the liberation degree of uranium-contaminated soil increased significantly after bacterial oxidation, which was conducive to the contact of bacteria and Fe3+ to soil particles, thus improving the uranium removal rate.
期刊：
Journal of Fluorescence,2022年32(5):1651-1660 ISSN：1053-0509
通讯作者：
Liang, Hao;Chen, Lili
作者机构：
[Bai, Qinqin; Liang, Hao; Liu, Luyao; Chen, Lili; Zhang, Xuebing] Univ South China, Dept Publ Hlth Lab Sci, Sch Publ Hlth, Hengyang Med Sch, Hengyang 421001, Hunan, Peoples R China.;[Bai, Qinqin; Liang, Hao; Liu, Luyao; Chen, Lili; Zhang, Xuebing] Univ South China, Hunan Key Lab Typical Environm Pollut & Hlth Haza, Sch Publ Hlth, Hengyang Med Sch, Hengyang 421001, Hunan, Peoples R China.;[Bai, Qinqin; Liang, Hao; Liu, Luyao; Chen, Lili; Zhang, Xuebing] Hengyang Engn Technol Res Ctr, Hengyang 421001, Hunan, Peoples R China.;[Li, Zhongyu; Lu, Chunxue] Univ South China, Hengyang Med Sch, Inst Pathogen Biol, Hengyang 421001, Hunan, Peoples R China.
通讯机构：
[Liang, Hao; Chen, Lili] D;Department of Public Health Laboratory Sciences, School of Public Health, Hengyang Medical School, University of South China, Hengyang, 421001, Hunan, China.;Hunan Key Laboratory of Typical Environmental Pollution and Health Hazards, School of Public Health, Hengyang Medical School, University of South China, Hengyang, 421001, Hunan, China.;Hengyang Engineering Technology Research Center, Hengyang, 421001, Hunan, China.
关键词：
Chlamydia trachomatis;CuNCs;Detection;Hairpin DNA
摘要：
Background: Ovarian serous cystadenocarcinoma (OSC), a common gynecologic tumor, is characterized by high mortality worldwide. Bromodomain (BRD)-containing proteins are a series of evolutionarily conserved proteins that bind to acetylated Lys residues of histones to regulate the transcription of multiple genes. The ectopic expression of BRDs is often observed in multiple cancer types, but the role of BRDs in OSC is still unclear. Methods: We performed the differential expression, GO enrichment, GSEA, immune infiltration, risk model, subtype classification, stemness feature, DNA alteration, and epigenetic modification analysis for these BRDs based on multiple public databases. Results: Most BRDs were dysregulated in OSC tissues compared to normal ovary tissues. These BRDs were positively correlated with each other in OSC patients. Gene alteration and epigenetic modification were significant for the dysregulation of BRDs in OSC patients. GO enrichment suggested that BRDs played key roles in histone acetylation, viral carcinogenesis, and transcription coactivator activity. Two molecular subtypes were classified by BRDs for OSC, which were significantly correlated with stemness features, m6A methylation, ferroptosis, drug sensitivity, and immune infiltration. The risk model constructed by LASSO regression with BRDs performed moderately well in prognostic predictions for OSC patients. Moreover, BRPF1 plays a significant role in these BRDs for the development and progression of OSC patients. Conclusion: BRDs are potential targets and biomarkers for OSC patients, especially BRPF1.
摘要：
BACKGROUND: Intracranial hypertension is a common clinicopathological syndrome in neurosurgery, and a timely understanding of the intracranial pressure (ICP) may help guide clinical treatment. We aimed to investigate the correlation between subharmonic contrast-enhanced ultrasound (SHCEUS) parameters and ICP in experimental canines. METHODS: A dynamic model of ICP change from 11 mm Hg to 50 mm Hg was established in experimental canines by placing a latex balloon into the epidural space and injecting saline into the balloon. In addition, a pressure sensor was placed in the brain parenchyma to record the changes in ICP. When the ICP stabilized after each increase, subharmonic-aided pressure estimation (SHAPE) technology was performed to obtain the SHCEUS parameters, including the basal venous and adjacent intracranial arterial subharmonic amplitude and SHAPE gradient (subharmonic amplitude in the intracranial artery minus that in the basal vein). The correlation between these parameters and ICP was analyzed. RESULTS: The subharmonic amplitude of the basal vein was negatively correlated with the ICP (r = -0.798), and the SHAPE gradient was positively correlated with the ICP (r = 0.628). According to the guidelines for ICP monitoring in patients with traumatic brain injury, we defined 20 mm Hg, 25 mm Hg, and 30 mm Hg as the cutoff ICP levels. The area under the receiver operating characteristic curve of the basal venous subharmonic amplitude for diagnosing intracranial hypertension ≥20 mm Hg, ≥25 mm Hg, and ≥30 mm Hg was 0.867 (95% confidence interval [CI], 0.750-0.943), 0.884 (95% CI, 0.770-0.954), and 0.875 (95% CI, 0.759-0.948), respectively. The area under the receiver operating characteristic curve of the SHAPE gradient for diagnosing intracranial hypertension ≥20 mm Hg, ≥25 mm Hg, and ≥30 mm Hg was 0.839 (95% CI, 0.716-0.924), 0.842 (95% CI, 0.720-0.926), and 0.794 (95% CI, 0.665-0.890), respectively. CONCLUSION: SHCEUS parameters are correlated with ICP. The SHAPE technique can assist in evaluating ICP changes in canines, which provides a new idea and method for evaluating ICP.
摘要：
In this paper, we investigate the innermost stable circular orbit (ISCO) of spinning particles around a rotating black hole surrounded by the perfect fluid dark matter (PFDM). The motion of spinning particles is derived by Mathisson–Papapetrou–Dixon equation and the ISCO parameters are obtained by the radial effective potential. The effect of PFDM density parameter on the motion of spinning particles is analyzed. The results show that the effect of PFDM is nonlinear, and the radius of ISCO varies nonmonotonically with PFDM density parameter.
作者机构：
[Zhu, Zi-Gui; He, Cheng-Jian; Liao, Gu-Qing] Univ South China, Affiliated Nanhua Hosp, Hengyang Med Coll, Dept Intens Care Unit, Hengyang 421002, Hunan, Peoples R China.;[Zhang, Jian-Xin] Univ South China, Affiliated Nanhua Hosp, Hengyang Med Coll, Dept Thorac & Cardiovasc Surg, Hengyang 421002, Hunan, Peoples R China.;[Ni, Zhi-Chao] Univ South China, Affiliated Nanhua Hosp, Hengyang Med Coll, Dept Thorac & Cardiovasc Surg, 336 Dongfeng South Rd, Hengyang 421002, Hunan, Peoples R China.
通讯机构：
[Zhi-Chao Ni] D;Department of Thoracic and Cardiovascular Surgery, The Affiliated Nanhua Hospital, Hengyang Medical College, University of South China, Hengyang 421002, Hunan Province, PR China
摘要：
BACKGROUND: Lung injury is a severe complication of sepsis, which brings great threats and challenges to human health. CircVMA21 has exhibited powerful anti- inflammation capacity. However, its underlying molecule mechanism remains blurry. METHODS: Lipopolysaccharide (LPS) was used to treat mice and WI-38 cells to establish models of lung injury caused by sepsis. Lung injury was evaluated using HE staining. Cell apoptosis was tested by TUNEL and flow cytometry. Levels of inflammatory cytokines were detected using ELISA assay. CircVMA21 and SOCS3 expression was measured using RT-qPCR. The ROS, MDA, SOD and GSH production were monitored by commercial kits. The protein expression was examined with western blot. The correlations among circVMA21, SOCS3 and TAF15 were confirmed using RIP and RNA-pull down. RESULTS: The expression of circVMA21 and SOCS3 was downregulated in LPS-induced lung injury of mice and WI-38 cells. Overexpressing circVMA21 or SOCS3 assuaged LPS-induced cell injury through repressing the levels of inflammatory factors, oxidative stress and cell apoptosis. NF-κB signaling pathway was inactivated by circVMA21 or SOCS3 overexpression. circVMA21 enhanced the stabilization of SOCS3 mRNA via interplaying with TAF15. SOCS3 knockdown destroyed the beneficial impacts of circVMA21 overexpression on LPS-induced cell injury. CONCLUSION: CircVMA21 suppressed LPS-induced the levels of inflammatory factors, oxidative stress and cell apoptosis and improved LPS-induced lung injury by mediating TAF15/SOCS3/NF-κB axis.
摘要：
Objectives: The contributing factors for spondylitis after percutaneous vertebroplasty (PVP) or percutaneous kyphoplasty (PKP) remain unclear. Here, we sought to investigate the factors affecting spondylitis occurrence after PVP/PKP. We also compared the clinical characteristics between patients with tuberculous spondylitis (TS) and nontuberculous spondylitis (NTS) following vertebral augmentation. Methods: Literature searches (from January 1, 1982 to October 16, 2020) using MEDLINE, EMBASE, Google Scholar and Web of science databases were conducted to identify eligible studies according to predefined criteria. The local database was also retrospectively reviewed to include additional TS and NTS patients at our center. Results: Thirty studies from the literature and 11 patients from our local institute were identified, yielding a total of 23 TS patients and 50 NTS patients for analysis. Compared with NTS group, patients in the TS group were more likely to have a history of trauma before PVP/PKP treatment. Univariate analyses of risk factors revealed pulmonary tuberculosis and diabetes were significant factors for TS after PVP/PKP. Analyzing NTS, we found obesity, a history of preoperative trauma, urinary tract infection, diabetes and multiple surgical segments (≥2) were significantly associated with its occurrence following PVP/PKP treatment. Multivariate logistic analyses showed a history of pulmonary tuberculosis and diabetes were independent risk factors for TS after PVP/PKP, while diabetes and the number of surgically treated segments independently influenced NTS development. Conclusions: A history of pulmonary tuberculosis and diabetes were independent risk factors for TS. For NTS, diabetes and the number of surgically treated segments significantly influenced the occurrence of postoperative spinal infection. These data may be helpful for guiding risk stratification and preoperative prevention for patients, thereby reducing the incidence of vertebral osteomyelitis after PVP/PKP. 2022 Zheng, Liu, Zheng, Niu, Li, Lv, Zou and Xu.
期刊：
BIOCHEMISTRY AND CELL BIOLOGY,2022年100(1):9-16 ISSN：0829-8211
通讯作者：
Zhang, C
作者机构：
[Yang, Chen; Li, Yong-Zhen; Qu, Shun-Lin; Zhang, Chi; Zhao, Yu-Yan; Chen, Lin-Hui] Univ South China, Hengyang Med Coll, Inst Cardiovasc Dis, Key Lab Arteriosclerol Hunan Prov, Hengyang 421001, Hunan, Peoples R China.;[Huang, Liang] Univ South China, Hengyang Med Coll, Res Lab Translat Med, Hengyang 421001, Hunan, Peoples R China.;[Zhu, Ying] Univ South China, Coll Publ Hlth, Dept Hlth Inspect & Quarantine, Hengyang 421001, Hunan, Peoples R China.
通讯机构：
[Zhang, C ] U;Univ South China, Hengyang Med Coll, Inst Cardiovasc Dis, Key Lab Arteriosclerol Hunan Prov, Hengyang 421001, Hunan, Peoples R China.
关键词：
glucagon-like peptide 1;type 2 diabetes;mitogen-activated protein kinase pathway;cardiovascular disease;peptide GLP-1;diabète de type2;voie de la MAPK;maladies cardiovasculaires
作者机构：
[Cao, Yi] Univ South China, Sch Publ Hlth, Hengyang Med Sch, Hunan Prov Key Lab Typ Environm Pollut & Hlth Haza, Hengyang 421001, Peoples R China.
通讯机构：
[Yi Cao] H;Hunan Province Key Laboratory of Typical Environmental Pollution and Health Hazards, School of Public Health, Hengyang Medical School, University of South China, Hengyang 421001, China
关键词：
3D human brain organoids;Neurotoxicity
摘要：
Neurotoxicity studies aim at understanding the toxic effects and mechanisms of toxicants to human central nervous systems (CNS). However, human brains are the most complex organs, whereas the most commonly used models, such as 2D cell cultures and animal brains, are probably too simple to predict the responses of human brains. Embryonic stem cells (ESCs) or induced pluripotent stem cells (iPSCs)-based 3D human brain organoids hold unprecedented promise for the understanding of neurodevelopment and brain disease development. This review summarizes recent advances of using 3D human brain organoids for neurotoxicity studies. Comparative studies showed that 3D human brain organoids could support the findings obtained by animal or cohort studies, indicating that 3D human brain organoids are reliable models to evaluate the developmental neurotoxicity. 3D human brain organoids have been used to understand the toxicological mechanisms by using both conventional toxicological methods to investigate the signaling pathway changes as well as single cell RNA-sequencing to understand the neuron diversity. Some studies also used brain organoids carrying gene mutations or with virus infections to understand the toxicological responses of brains under diseased conditions. Although there are still limitations associated, 3D human brain organoids are promising tools for future neurotoxicity studies.
期刊：
Biochemical and Biophysical Research Communications,2022年591:7-12 ISSN：0006-291X
通讯作者：
Yao, Maojin
作者机构：
[Gong, Congcong; Ren, Jiaoyan] South China Univ Technol, Sch Food Sci & Technol, Guangzhou 510641, Guangdong, Peoples R China.;[Zhou, Mao] Sun Yat Sen Univ, Sun Yat Sen Mem Hosp, Dept Anesthesiol, Guangzhou 510120, Guangdong, Peoples R China.;[Yao, Maojin; Hu, Yang] Guangzhou Med Univ, Affiliated Hosp 1, Guangzhou, Guangdong, Peoples R China.;[Ren, Zhengyu] Univ South China, Sch Pharmaceut Sci, Hengyang 421001, Hunan, Peoples R China.;[Yao, Maojin] Guangzhou Med Univ, Affiliated Hosp 1, State Key Lab Resp Dis, Guangzhou Inst Resp Hlth, Guangzhou, Guangdong, Peoples R China.
通讯机构：
[Yao, Maojin] G;Guangzhou Med Univ, Affiliated Hosp 1, State Key Lab Resp Dis, Guangzhou Inst Resp Hlth, Guangzhou, Guangdong, Peoples R China.
关键词：
*Cross-validated Cox proportional hazards model;*Diagnostic marker;*Early-stage gastric cancer
摘要：
Early-stage gastric cancer (GC) is asymptomatic. How to diagnose the early-stage GC is challenging. The sensitivity and specificity of diagnosing signatures for early-stage patients are still poor. Elastic-net based analysis was used to identify potential diagnostic signatures of early-stage GC. The expression level of candidate gene was evaluated by immunohistochemistry staining. The potential function of candidate gene was verified by overexpressing in vitro. Consensus genes (including GAMT) were identified using the different strengths of the penalty. Surprisingly, GAMT was still identified even if some multicollinear variables were deleted directly. IHC staining showed that there are no GAMT-positive signals in the cell nuclei of all tumor tissues, while GAMT does express in nuclei of adjacent normal tissue. There are 16.33% positive cell nuclei in paracancerous tissues. In addition, the number of larger area colonies of overexpression-GAMT group, empty-vector group, and AGS group is 70 +/- 29.21, 151.33 +/- 15.95, and 111.67 +/- 22.03, respectively. Number of larger colonies in group with overexpression of GAMT is significantly less than control groups. Elastic-net-penalty-based workflow is a effective tool to identify diagnostic biomarker for early-stage solid tumor. GAMT has strong potential to be the diagnostic biomarker for the early-stage GC. (c) 2021 Published by Elsevier Inc.
作者机构：
[Gong, Jianye; Li, Chunbin; Jiang, Guoyu; Wang, Jianguo] Inner Mongolia Univ, Coll Chem & Chem Engn, Inner Mongolia Key Lab Fine Organ Synth, Hohhot 010021, Peoples R China.;[Qin, Anjun; Hu, Rong] South China Univ Technol, State Key Lab Luminescent Mat & Devices, Guangzhou 510640, Peoples R China.;[Lam, Jacky W. Y.] Hong Kong Univ Sci & Technol, Chinese Natl Engn Res Ctr Tissue Restorat & Recon, Hong Kong Branch, Kowloon, Clear Water Bay, Hong Kong, Peoples R China.;[Tang, Ben Zhong] Chinese Univ Hong Kong Shenzhen, Shenzhen Inst Aggregate Sci & Technol, Sch Sci & Engn, Shenzhen 518172, Guangdong, Peoples R China.;[Hu, Rong] Univ South China, Sch Chem & Chem Engn, Hengyang 421001, Peoples R China.
通讯机构：
[Prof. Dr. Jianguo Wang] C;[Prof. Dr. Anjun Qin; Prof. Dr. Ben Zhong Tang] S;School of Science and Engineering, Shenzhen Institute of Aggregate Science and Technology, The Chinese University of Hong Kong Shenzhen, Guangdong, 518172 P. R. China<&wdkj&>State Key Laboratory of Luminescent Materials and Devices, South China University of Technology, Guangzhou, 510640 P. R. China<&wdkj&>College of Chemistry and Chemical Engineering, Inner Mongolia Key Laboratory of Fine Organic Synthesis, Inner Mongolia University, Hohhot, 010021 P. R. China
