期刊：
Molecular and Cellular Biochemistry,2014年392(1-2):153-159 ISSN：0300-8177
通讯作者：
He, Xiusheng
作者机构：
[Zhang, Zhiwei; He, Xiusheng; Xie, Liming; Xie, Yuanjie; Tang, Guohua; Zeng, Xi; Li, Suyun] Univ South China, Canc Res Inst, Key Lab Canc Cellular & Mol Pathol Hunan Prov Uni, Hengyang 421001, Hunan, Peoples R China.;[Tan, Zhiqin; Xie, Liming; He, Rongfang] Univ South China, Affiliated Hosp 1, Ctr Gastr Canc Res Human Prov, Hengyang 421001, Hunan, Peoples R China.;[Tang, Hailin] Sun Yat Sen Univ, Collaborat Innovat Ctr Canc Med, State Key Lab Oncol South China, Ctr Canc, Guangzhou 510060, Guangdong, Peoples R China.;[He, Xiusheng] Univ South China, Canc Res Inst, Key Lab Canc Cellular & Mol Pathol Hunan Prov Uni, 28 West Changsheng Rd, Hengyang 421001, Hunan, Peoples R China.
通讯机构：
[He, Xiusheng] U;Univ South China, Canc Res Inst, Key Lab Canc Cellular & Mol Pathol Hunan Prov Uni, 28 West Changsheng Rd, Hengyang 421001, Hunan, Peoples R China.
关键词：
Gastric cancer;miR-124;EZH2;Proliferation
摘要：
MicroRNA-124 (miR-124), a pivotal member of the p53 network, was found to be down-regulated in multiple types of tumors and further reported as tumor suppressor microRNA. In this study, we found that miR-124 was down-regulated in gastric cancer cell lines and specimens. Restoration of miR-124 expression inhibited the proliferation and colony formation of gastric cancer cells. EZH2 (enhancer of zeste homolog 2), which has been shown to be an important transcription factor involved in the proliferation and metastasis of tumor cells, was here confirmed to be a direct target gene of miR-124. On the other hand, silencing EZH2 also inhibits cell proliferation of gastric cancer cells. Furthermore, the treatment combining miR-124 with 5-fluorouracil (5-FU) significantly showed more efficient anti-tumor effects than single treatment of miR-124 or 5-FU, and over-expression of miR-124 suppresses the tumor growth in vivo. Our study indicate that miR-124 can suppress gastric cancer cell growth by directly targeting the EZH2 gene and sensitize the treatment effect of 5-FU. Therefore, miR-124 shows tumor-suppressive activity and may be a new and useful approach of gastric cancer therapy.
作者机构：
[Zhi-Wei Zhang; Xiu-Sheng He] Cancer Research Institute, University of Southern China;[Zhi-Wei Zhang; Xiu-Sheng He] University Key Laboratory of Cancer Cellular and Molecular Pathology in Hunan Province
会议名称：
第八届中国肿瘤学术大会暨第十三届海峡两岸肿瘤学术会议
会议时间：
2014-09-11
会议地点：
济南
会议论文集名称：
第八届中国肿瘤学术大会暨第十三届海峡两岸肿瘤学术会议论文集
摘要：
<正>To discover novel biomarkers for early detection of human gastric adenocarcinoma(GAC)and exPlore Possible mechanisms of GAC carcinogenesis,iTRAQ-tagging combined with two dimensional liquid chromat
摘要：
The definite molecular mechanisms underlying the genesis of nasopharyngeal carcinomas (NPCs) remain to be completely elucidated. miRNAs are small non-coding RNAs which are implicated in cell proliferation, apoptosis, and even carcinogenesis through negatively regulating gene expression post-transcriptionally. EBV was the first human virus found to express miRNAs. EBV-encoded BART-miRNAs and dysregulated cellular miRNAs are involved in carcinogenesis of NPC by interfering in the expression of viral and host cell genes related to immune responses and perturbing signal pathways of proliferation, apoptosis, invasion, metastasis and even radio-chemo-therapy sensitivity. Additional studies on the roles of EBV-encoded miRNAs and cellular miRNAs will provide new insights concerning the complicated gene regulated network and shed light on novel strategies for the diagnosis, therapy and prognosis of NPC.
作者机构：
[Zheng, Yi; Yi, Jia; George, Alfred L., Jr.; Yi, Yajun; Xiang, Yuzhu; Qiu, Qingchao; Yousif, Dina A.; Simmons, Christine Q.] Vanderbilt Univ, Dept Med, Nashville, TN 37232 USA.;[Zheng, Yi; Jin, Xunbo; Xiang, Yuzhu] Shandong Univ, Prov Hosp, Minimally Invas Urol Ctr, Jinan 250021, Peoples R China.;[Jin, Renjie; Jiang, Ming; Hayward, Simon W.; Matusik, Robert J.; Case, Thomas C.; Strand, Douglas W.; DeGraff, David J.] Vanderbilt Univ, Vanderbilt Prostate Canc Ctr, Nashville, TN 37232 USA.;[Jin, Renjie; Jiang, Ming; Hayward, Simon W.; Matusik, Robert J.; Case, Thomas C.; Strand, Douglas W.; DeGraff, David J.] Vanderbilt Univ, Dept Urol Surg, Nashville, TN 37232 USA.;[George, Alfred L., Jr.; Yi, Yajun] Vanderbilt Univ, Inst Integrat Gen, Nashville, TN 37232 USA.
通讯机构：
[Yi, Yajun] V;Vanderbilt Univ, Div Med Genet, 536A Light Hall,2215 Garland Ave, Nashville, TN 37232 USA.
关键词：
Prostate cancer;Gene expression signature;Meta-analysis;Metastasis;SPARCL1 function in vivo
摘要：
Metastasis, the main cause of death from cancer, remains poorly understood at the molecular level.
Based on a pattern of reduced expression in human prostate cancer tissues and tumor cell lines, a candidate suppressor gene (SPARCL1) was identified. We used in vitro approaches to determine whether overexpression of SPARCL1 affects cell growth, migration, and invasiveness. We then employed xenograft mouse models to analyze the impact of SPARCL1 on prostate cancer cell growth and metastasis in vivo.
SPARCL1 expression did not inhibit tumor cell proliferation in vitro. By contrast, SPARCL1 did suppress tumor cell migration and invasiveness in vitro and tumor metastatic growth in vivo, conferring improved survival in xenograft mouse models.
We present the first in vivo data suggesting that SPARCL1 suppresses metastasis of prostate cancer.
摘要：
STGC3 is a potential tumor suppressor in nasopharyngeal carcinoma. We previously found that CNE2 cells that re-expressed STGC3 formed smaller tumors in female mice than in male mice. Here, we investigated the sexual dimorphism of STGC3 as a tumor-suppressor in female and male nude mice injected subcutaneously with pcDNA3.1(+)-STGC3/CNE2 cells. ER-α was positively expressed in vitro in the CNE2 cells. The pcDNA3.1(+)-STGC3/CNE2 cell growth rate decreased after treatment with β-estradiol in vitro. There were significant differences in tumor size or mass between pcDNA3.1(+)-STGC3/CNE2 and control cases (P < 0.05), but there were significant differences in tumor size between female and male nude mice in the STGC3 transfection groups, and the pcDNA3.1(+)-STGC3/CNE2 tumor growth rate in the female nude mice was the lowest in all cases (P < 0.05). There were no significant differences between female and male nude mice in control groups. Furthermore, a greater number of cells were blocked in the G(0)/G(1) phase in pcDNA3.1(+)-STGC3/ CNE2 tumor xenografts in the female mice. Protemic analysis found 9 differentially expressed proteins in the pcDNA3.1-STGC3/CNE2 xenograft tissues in females and males. A heat shock 70 protein 8 isoform 2 variant was identified as a down-regulated protein associated with cell cycle control and its downstream factor cyclin D1 was also decreased in STGC3-repressed xenografts in female mice. The data above suggest that STGC3 and its associated proteins play an important role in nasopharyngeal carcinoma gender differences.
作者机构：
[唐国华; 孙少卫] Research Center of Life Science, South China University, Hengyang, 421001, China;[赵强] Department of Pathology, First Affiliated Hospital, South China University, Hengyang, 421001, China;[贺修胜] Cancer Research Institute of Medical College, South China University, Hengyang, 421001, China
通讯机构：
[Tang, G.-H.] R;Research Center of Life Science, South China University, Hengyang, China
