期刊：
JOURNAL OF INFECTIOUS DISEASES,2006年193(7):1037-1046 ISSN：0022-1899
通讯作者：
Wong, BCY
作者机构：
[Wong, BCY] Univ Hong Kong, Queen Mary Hosp, Dept Med, Hong Kong, Hong Kong, Peoples R China.;Univ Hong Kong, Dept Pharmacol, Hong Kong, Hong Kong, Peoples R China.;Sun Yat Sen Univ, Affiliated Hosp 1, Dept Med, Guangzhou, Peoples R China.;Sun Yat Sen Univ, Dept Pathol, Guangzhou, Peoples R China.;Nanhua Univ, Affiliated Hosp 2, Dept Med, Hengyang, Peoples R China.
通讯机构：
[Wong, BCY] U;Univ Hong Kong, Queen Mary Hosp, Dept Med, Hong Kong, Hong Kong, Peoples R China.
会议名称：
Digestive Disease Week Meeting/105th Annual Meeting of the American-Gastroenterological-Association
会议时间：
MAY 16-20, 2004
会议地点：
New Orleans, LA
会议主办单位：
Univ Hong Kong, Queen Mary Hosp, Dept Med, Hong Kong, Hong Kong, Peoples R China.^Univ Hong Kong, Dept Pharmacol, Hong Kong, Hong Kong, Peoples R China.^Sun Yat Sen Univ, Affiliated Hosp 1, Dept Med, Guangzhou, Peoples R China.^Sun Yat Sen Univ, Dept Pathol, Guangzhou, Peoples R China.^Nanhua Univ, Affiliated Hosp 2, Dept Med, Hengyang, Peoples R China.^Washington Univ, Dept Mol Microbiol, St Louis, MO 63110 USA.^Univ Texas, MD Anderson Canc Ctr, Dept Expt Therapeut, Houston, TX 77030 USA.^Natl Inst Environm Hlth Sci, Res Triangle Pk, NC USA.
作者机构：
Nanhua Univ, Coll Med, Canc Res Inst, Hengyang, Hunan Province, Peoples R China.;Hunan Normal Univ, Coll Live Sci, Changsha, Hunan Province, Peoples R China.
作者机构：
[Feng, JQ] Sun Yat Sen Univ, Zhongshan Med Coll, Dept Physiol, Guangzhou 510080, Peoples R China.;Nanhua Univ, Coll Med, Dept Physiol, Hengyang City 421001, Hunan, Peoples R China.
通讯机构：
[Feng, JQ] S;Sun Yat Sen Univ, Zhongshan Med Coll, Dept Physiol, Guangzhou 510080, Peoples R China.
关键词：
Apoptosis;Bcl-2;Hydrogen peroxide;Mitochondrial membrane potential;PC12 cell;Preconditioning;Reactive oxygen species
关键词：
apoptosis;Carrier Proteins;Chlamydia trachomatis;membrane proteins;Proto-Oncogene Proteins;Tumor Suppressor Protein p53
摘要：
Volume 73, no. 3, p. 1861-1864, 2005. Page 1863, References: During the revision of our paper, a report describing similar findings was published and should have been cited.
6a. Fischer, S. F., J. Vier, S. Kirschnek, A. Klos, S. Hess, S. Ying, and G. Hacker. 2004. Chlamydia inhibit host cell
摘要：
AIM: To define the infection status of Helicobacter pylori in 109 patients with gastric cancers and H pylori localization in gastric carcinoma tissues in South China. METHODS: The incidence of H pylori infection in gastric carcinomas was estimated by polymerase chain reaction (PCR), simultaneously; both morphological features and the localization of H pylori in gastric carcinomas were demonstrated by Warthin-Starry (WS) staining. The relationships between H pylori infection and the clinical-pathologic factors of gastric carcinomas were analyzed by software SPSS10.0. RESULTS: H pylori was found in 42 (39.03%) and 58 (53.21%) cases of 109 patients with gastric carcinomas by PCR and WS, respectively. H pylori infection rate detected in gastric carcinomas by WS was higher than that by PCR (chi(2) = 9.735, P < 0.005 < 0.01). WS stain showed that H pylori existed in the gastric antrum mucus, mucosal gland of normal tissues adjacent to gastric carcinomas and the gland, mucus pool of cancer tissues. The positive rate of H pylori in normal tissues adjacent to carcinomas was higher than that in cancer tissues (chi(2) = 15.750, P < 0.005 < 0.01). No significant differences in age, sex, site, histological types and lymph node metastasis were found between H pylori-positive gastric carcinomas and H pylori-negative cases by both methods, but there were statistically significant differences of H pylori positive rate between early and advanced stage of gastric carcinomas (chi(2) = 4.548 or 5.922, P = 0.033 or 0.015 < 0.05). CONCLUSION: These results suggested that H pylori infection might play a certain role in the early stage of carcinogenesis of human gastric mucosa epithelia. (C) 2005 The WJG Press and Elsevier Inc. All rights reserved.
摘要：
AIM: To analyze the correlation between the protein expression of p16 and Rb genes in gastric carcinoma (GC), to investigate the role of p16 gene in invasion and lymph node metastasis of GC, and to examine the deletion and mutation in exon 2 of p16 gene in GC. METHODS: The protein expression of p16 and Rb genes was examined by streptavidin-peroxidase conjugated method (S-P) in normal gastric mucosa, dysplastic gastric mucosa and GC. The deletion and mutation of p16 gene were examined by polymerase chain reaction (PCR) and polymerase chain reaction single strand conformation polymorphism (PCR-SSCP) respectively in normal gastric mucosa and GC. RESULTS: The positive rates of P16 and Rb protein expression respectively were 96% (77/80) and 99% (79/80) in normal gastric mucosa, 92% (45/50) and 80% (40/50) in dysplastic gastric mucosa, 48% (58/122) and 60% (73/122) in GC. The positive rates of P16 and Rb protein expression in GC were significantly lower than that in normal gastric mucosa and dysplastic gastric mucosa (P<0.05). The positive rate of P16 protein expression in mucoid carcinoma (10%, 1/10) was significantly lower than that in poorly differentiated carcinoma (51%, 21/41), undifferentiated carcinoma (58%, 15/26) and signet ring cell carcinoma (62%, 10/16) (P<0.05). The positive rates of P16 protein in 30 cases of paired primary and lymph node metastatic GC were 47% (14/30) and 17% (5/30) respectively, being significantly lower in the later than in the former (P<0.05). There was no mutation in exon 2 of p16 gene in the 25 freshly resected primary GCs. But five cases in the 25 freshly resected primary GCs displayed deletion in exon 2 of p16 gene. The positive rate of both P16 and Rb proteins was 16% (14/90), and the negative rate of both P16 and Rb proteins was 8% (7/90) in 90 GCs. The rate of positive P16 protein with negative Rb protein was 33% (30/90). The rate of negative P16 protein with positive Rb protein was 43% (39/90). There was reverse correlation between P16 and Rb expression in 90 GCs (P<0.05). CONCLUSION: The loss protein expression of p16 and Rb genes is related to GC. The loss expression of P16 protein is related to the histopathologic subtypes and lymph node metastasis of GC. Expression of P16 and Rb proteins in GC is reversely correlated. The deletion but not mutation in exon 2 of p16 gene may be involved in GC. (C) 2005 The WJG Press and Elsevier Inc. All rights reserved.
摘要：
HLCDG1, which locates in chromosome 5q33, is a novel gene cloned recently. The HLCDG1 expression was significantly down regulated in the primary lung carcinoma. It was previously studied that HLCDG1 acted like a tumor suppressor gene. In this paper, proteomics studies were performed to analyze the proteomic expression patterns in the HLCDG1-transfected human lung carcinoma cell line (A549-HLCDG1) and in the control vector-transfected human lung carcinoma cell line (A549-vector). Employing two dimensional gel electrophoresis (2DE), the global pattern of protein expressions in A549-HLCDG1 human lung adenocarcinoma cell line expressing stably HLCDG1 gene were compared with those of control A549-vector cell line to generate a differential protein expression catalog. Forty-two differentially expressed proteins were screened. Thirteen differential proteins were identified by matrix-assisted laser desorption/ ionization time of flight mass spectrometry (MALDI-TOF-MS), which were 6 upregulated (MSH5, MOD, MDH precursor, ETF beta, Prxd VI and JM23) and 7 downregulated (PLC-delta 1, hnRNPA2, hnRNPB1, TIM, TCTP, nm23H- 1 and PrxdV) proteins in A549-HLCDG1 cells compared to control A549-vector cells. The above identified proteins were involved in energy metabolism, transcription regulation, antioxidation, cell cycle, metastasis, DNA methylation and mismatch repair. Therefore. these differential expression proteins by HLCDG1 transfection may play some important roles for investigation of the biochemical basis of growth suppression of HLCDG1 gene in lung carcinoma cells A549. Further understanding of this data base may provide valuable resources for the developing novel diagnostic markers and therapeutic targets of lung cancer.
关键词：
apoptosis;Carrier Proteins;Chlamydia trachomatis;membrane proteins;Proto-Oncogene Proteins;Tumor Suppressor Protein p53
摘要：
We have previously correlated Chlamydia trachomatis antiapoptotic activity with the blockade of mitochondrial cytochrome c release and the inhibition of Bax and Bak activation. We now report that C. trachomatis infection leads to degradation of Bik, Puma, and Bim, three upstream proapoptotic BH3-only proteins of the Bcl-2 family that can transmit death signals to mitochondria by inhibiting the Bcl-2 antiapoptotic proteins and/or activating the Bcl-2 proapoptotic members, such as Bax and Bak. This observation has provided new information on the chlamydial antiapoptosis mechanisms.
期刊：
Russian Journal of Genetics,2005年41(7):755-759 ISSN：1022-7954
通讯作者：
Zheng, JF
作者机构：
[Zheng, JF] Nanhua Univ, Coll Life Sci & Technol, Inst Cell & Genet, Hengyang 421001, Peoples R China.;Nanhua Univ, Coll Med, Inst Physiol, Hengyang 421001, Peoples R China.;Nanhua Univ, Coll Life Sci & Technol, Inst Zool, Hengyang 421001, Peoples R China.
通讯机构：
[Zheng, JF] N;Nanhua Univ, Coll Life Sci & Technol, Inst Cell & Genet, Hengyang 421001, Peoples R China.
关键词：
Rainbow Trout;Homologous Sequence;Amino Acid Identity;Acid Identity;Equivalent Region
摘要：
In order to understand sequence information about turtle HMG1 gene, a cDNA encoding HMG1 protein of the Chinese soft-shell turtle (Pelodiscus sinensis) was amplified by RT-PCR from kidney total RNA, and was cloned, sequenced and analyzed. The results revealed that the open reading frame (ORF) of turtle HMG1 cDNA is 606 bp long. The ORF codifies 202 amino acid residues, from which two DNA-binding domains and one polyacidic region are derived. The DNA-binding domains share higher amino acid identity with homologues sequences of chicken (96.5%) and mammalian (74%) than homologues sequence of rainbow trout (67%). The polyacidic region shows 84.6% amino acid homology with the equivalent region of chicken HMG1 cDNA. Turtle HMG1 protein contains 3 Cys residues located at completely conserved positions. Conservation in sequence and structure suggests that the functions of turtle HMG1 cDNA may be highly conserved during evolution. To our knowledge, this is the first report of HMG1 cDNA sequence in any reptilian.
作者机构：
[Feng, JQ] Sun Yat Sen Univ, Zhongshan Med Coll, Dept Physiol, Guangzhou 510080, Peoples R China.;Nahua Univ, Coll Med, Dept Physiol, Hengyang City 421001, Hunan Province, Peoples R China.
通讯机构：
[Feng, JQ] S;Sun Yat Sen Univ, Zhongshan Med Coll, Dept Physiol, Guangzhou 510080, Peoples R China.
摘要：
Dopamine (DA), one of the major sources of reactive oxygen species (ROS), is implicated in neuronal death associated with Parkinson's disease (PD). Preconditioning with oxidative stress has been shown to provide cytoprotection similar to ischemic Preconditioning (IPC), against cell apoptosis. In this study, using the model neurosecretory cell line, PC12, we investigated whether hydrogen peroxide (H2O2) at low concentration (10 mu M) can protect PC12 cells against apoptosis induced by DA. PC12 cells were preconditioned with 10 mu M H2O2 for 90 min, followed by 24-h recovery and subsequent exposures to different concentrations (20, 50 100 and 200 mu M) of DA for 24-h, respectively. DA induced apoptotic cell death with significant morphological nuclear changes and DNA fragmentation as well as the dysfunction of mitochondria. Preconditioning with H2O2 at 10 mu M significantly reduced the percentage of apoptotic cells and partly blocked the decreases in 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) reduction and mitochondrial membrane potential (MMP) induced by DA. These results suggest that preconditioning with low concentration of H2O2 protected PC12 cells against DAinduced apoptosis, the part restoration of the damaged mitochondrial functions might be one of the underlying mechanisms of this cytoprotection. (c) 2005 Elsevier Inc. All rights reserved.
