作者机构：
[姚碧云; 傅娟玲; 赵鹏; 袁准; 周宗灿] Department of Toxicology, Health Science Center, Peking University, Beijing 100191, China;Institute of Environmental Medicine and Radiation Hygiene, University of South China, Hengyang 421001, China;[应贤平; 仲伟鉴] Department of Toxicology, Shanghai Municipal Center for Disease Control and Prevention, Shanghai 200336, China;[张朝晖] Department of Toxicology, Health Science Center, Peking University, Beijing 100191, China, Institute of Environmental Medicine and Radiation Hygiene, University of South China, Hengyang 421001, China
通讯机构：
[Zhao, P.] D;Department of Toxicology, Health Science Center, Peking University, China
摘要：
OBJECTIVE: To investigate the nuclear proteomes in mitochondrial DNA (mtDNA)-depleted A549 cells (Rho0 cells) and their parental cells (Rho+ cells), and to learn more about the nuclear responses to mitochondrial dysfunction. METHODS: The nuclear proteomes of Rho and Rho + cells were characterized by two dimensional electrophoresis (2-DE) and SELDI-TOF ProteinChip technologies, the differentially expressed protein-spots were identified by MALDI-TOF mass spectrum (MS), the nucleophosmin and P53 expression were detected by Western blotting assay, and the mitochondrial membrane potential (MMP) was measured by the laser scanning confocal microscope. RESULTS: 2-DE results showed 11 protein-spots were down-regulated and 21 protein-spots were up-regulated in Rho0 cell nuclei. SELDI-TOF MS analysis with NP20 ProteinChips revealed 4 protein-peaks decreased in Rho0 cell nuclei. One down-regulated protein-spot was identified as eIF-6, and 4 up-regulated protein-spots were identified as nucleophosmin, SFRS1, SFRS3 and hnRNP G, respectively. The increased expression of nucleophosmin in Rho0 cells was verified by Western blotting. Based on the clues from proteomic analysis, P53 expression in Rho0 cells was higher than in Rho+ cells, and MMP was consistent in Rho+ and Rho0 cells. CONCLUSION: mtDNA-depletion induces nuclear proteome alteration. Rho0 cells can be used as a model to study the cross-talk between mitochondrion and nucleus.
作者机构：
[谢超; 李礼; 徐龙君] State Key Laboratory of Coal Mine Disaster Dynamics and Control, Chongqing University, Chongqing 400044, China;[李礼] Environmental Monitoring Center of Chongqing, Chongqing 401147, China;[谢超] School of Environmental Protection and Safety Engineering, University of South China, Hengyang 421001, China
通讯机构：
State Key Laboratory of Coal Mine Disaster Dynamics and Control, Chongqing University, China
作者机构：
[蒋复量; 潘东; 周科平; 邓红卫; 李魁; 刘高] School of Resources and Safety Engineering, Central South University, Changsha 410083, China;[蒋复量] School of Environment Protection and Safety Engineering, University of South China, Hengyang 421001, China
通讯机构：
School of Resources and Safety Engineering, Central South University, China
摘要：
The stabilization of slopes by placing passive piles is one of the innovative slope reinforcement techniques in recent years. There are numerous empirical and numerical methods for designing stabilizing piles. They can generally be classified into two different types: (1) pressure/displacement-based methods; (2) finite element/finite difference methods. However, seldom studies have been done on the stratified rock slope reinforced by piles, so in the present paper, the numerical simulation software FLAC3D is adopted to model the stratified rock slope, then the reinforced effect like deformation and stress of slope are studied, showing that if the pile is driven at the mid-bottom place of slope surface, the effect of controlling deformation of rock mass is the best. With increase of the length of pile, the maximum displacement of slope is decreased gradually.
期刊：
2011 International Conference on Remote Sensing, Environment and Transportation Engineering,2011年:3925-3929
作者机构：
[Junwen Lv; Fei Jia; Xiaowen Zhang] School of Environmental Protection and Safety Engineering, University of South China, Hengyang, China;[Zhigang Feng] School of Nuclear Resource and Nuclear Fuel Engineering, University of South China, Hengyang, China
摘要：
Column experiments on remediation of acid seepage water from U-containing debris using slated lime and silica sand mixture as PRB material have been done. The results showed that those mixture good results on treatment of acid seepage water. 1 unit volume mixture can neutralize 108.3, 53.5 and 45.9 unit volume acid seepage water of pH 3.12 with 1:5, 1:7 and 1:9 slated lime to sand mass Ratio, respectively. They also showed good results on U removal with less than 0.05 mg/L uranium concentration in filtrate when pH was no less than 6.5 and fade removing results of sulfate radical. The permeability of the mixture with 1:5 mass ratio was decreased from 10.9 m/d to 6.1 m/d, but the other two had no obviously change.
期刊：
Applied Mechanics and Materials,2011年71-78:3197-3200 ISSN：1660-9336
通讯作者：
Wang, Shuyun
作者机构：
[Wang, Shuyun] Univ South China, Sch Environm Protect & Safety Engn, Hengyang 421001, Peoples R China.;[Xie, Xionggang] Guizhou Univ, Min Coll, Guiyang 550004, Peoples R China.;[Chen, Xi] Univ South China, Chuanshan Coll, Hengyang 421001, Peoples R China.
通讯机构：
[Wang, Shuyun] U;Univ South China, Sch Environm Protect & Safety Engn, Hengyang 421001, Peoples R China.
会议名称：
International Conference on Green Building, Materials and Civil Engineering (GBMCE 2011)
会议时间：
AUG 22-23, 2011
会议地点：
Shangri La, PEOPLES R CHINA
会议主办单位：
[Wang, Shuyun] Univ South China, Sch Environm Protect & Safety Engn, Hengyang 421001, Peoples R China.^[Xie, Xionggang] Guizhou Univ, Min Coll, Guiyang 550004, Peoples R China.^[Chen, Xi] Univ South China, Chuanshan Coll, Hengyang 421001, Peoples R China.
会议论文集名称：
Applied Mechanics and Materials
关键词：
Computer Aided Design (CAD);Excavation;Numerical Simulation;Stratified Rock Mass;Tunnel
摘要：
Stratified rock mass is widely existing in tunnel engineering. The most relevant feature of stratified rocks is the occurrence of very persistent bedding, which makes the rock-mass highly non-isotropic. A number of techniques for designing underground excavations in stratified media have been described in the literature, like theoretical method and laboratory test, which can only be applied in analyzing the problem with simple geometry and costs much expense. Recently, with rapid development of computer technique, numerical simulation methods have been widely applied in engineerin. Among all the numerical simulation methods, fast lagrangian explicit finite difference code of continua (FLAC3D) is widely used to solve practical problems, especially in field of elasto-plastic characteristic, large deformation analysis and construction procedure. So in the present paper, numerical simulation for the failure mode of stratified rock mass after tunnel excavation is done by FLAC3D, which can give further guidance to understand the anisotropic characteristic of stratified rock mass.
摘要：
Through the analysis of characteristics and laws of the radiant hazards in uranium waste rock and tailings to the environment, decommissioning the present situation of uranium mining and milling facilities in our country, and combining with the main research work, this article makes a series of management strategies.
摘要：
The water-based Fe3O4 magnetic fluids were prepared by chemical co-precipitation of Fe2+ and Fe3+ using NaOH to control the pH, different surfactants were added to modify the magnetic particles. The stability, viscosity of the fluids and the magnetic property of magnetic particles were evaluated. The influences of different factors, such as reacting time, temperature, stirring speed, pH value, supersonic dispersing time, molar ratio of Fe2+/Fe3+, and surfactant, on properties of magnetic fluids were discussed. The experimental results showed that the optimal conditions were: molar ratio of Fe2+ and Fe3+ 1:1.5, pH value 9.0, stirring speed 1500 r.min(-1), reacting time 10 minutes, temperature 55 degrees C, supersonic dispersing time 30 minutes, suitable surfactant Tween 80.
