作者机构：
[Xu, Xiaona; Liang, Yizeng] Cent S Univ, Coll Chem & Chem Engn, Res Ctr Modernizat Chinese Tradit & Herbal Drug M, Changsha 410083, Hunan, Peoples R China.;[Xu, Xiaona] Univ S China, Coll Chem & Chem Engn, Hengyang, Hunan, Peoples R China.;[Tang, Zhonghai] Hunan Agr Univ, Biosci & Biotechnol Coll, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Liang, Yizeng] C;Cent S Univ, Coll Chem & Chem Engn, Res Ctr Modernizat Chinese Tradit & Herbal Drug M, Changsha 410083, Hunan, Peoples R China.
摘要：
A new spectrophotometric method for the determination of nicotine in mixtures without pre-separation has been proposed. Nicotine could react with 2,4-dinitrophenol through a charge-transfer reaction to form a colored complex. The second-order data from the visible absorption spectra of the complex in a series of ethanol–water binary solvents with various water volume fractions could be expressed as the combination of two bilinear data matrices. With the bilinear model, the second-order spectra data of mixtures containing nicotine and other interferents could be analysed by using second-order calibration algorithms, and the determination of nicotine in the mixtures could be achieved. The algorithm used here was parallel factor analysis. The method has been successfully used to determine nicotine in tobacco samples with satisfactory results.
摘要：
The title compound, C21H30O, was isolated from the soft coral Sinularia sp. The molecule contains four alicyclic rings, all trans-fused, among which three six-membered rings are in different distorted chair conformations while a five-membered ring assumes an envelope form.
摘要：
Forkhead box protein A1 (FoxA1) is an evolutionarily conserved winged helix transcription factor with diverse regulatory functions. However, little is known about the role of FoxA1 in acute lung injury (ALI) and pulmonary cell injury. In this study, an in vivo model was employed whereby rats were administered an intravenous injection of oleic acid (OA, 0.1 ml/kg), and alveolar type II epithelial cells (AT-2 cells) injury was induced by hydrogen peroxide (H2O2) in vitro. OA injection resulted in lung injury and AT-2 cells apoptosis in vivo. OA injection and H2O2 upregulated FoxA1 mRNA and protein in lung tissue of the in vivo ALI model and in H2O2 challenged AT-2 cells. Overexpression of FoxA1 promoted apoptosis, whereas FoxA1 deficiency, induced by antisense oligonucleotides, decreased AT-2 cells apoptosis induced by H2O2, as shown by flow cytometry. These results suggest that FoxA1 may play an important role in ALI by promoting apoptosis of pulmonary epithelial cells.
期刊：
International Journal of Mass Spectrometry,2009年286(2-3):112-121 ISSN：1387-3806
通讯作者：
Liang, Yi-Zeng
作者机构：
[Ouyang, Yong-Zhong; Xu, Xiaona; Zhang, Liangxiao; Luo, Xiao; Liang, Yi-Zeng; Tang, Zhonghai] Cent S Univ, Coll Chem & Chem Engn, Res Ctr Modernizat Chinese Med, Changsha 410083, Peoples R China.;[Li, Shuhua] Nanjing Univ, Key Lab Mesoscop Chem MOE, Dept Chem, Inst Theoret & Computat Chem, Nanjing 210093, Peoples R China.;[Tang, Zhonghai] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.;[Xu, Xiaona] Univ S China, Coll Chem & Chem Engn, Hengyang 421001, Hunan, Peoples R China.
通讯机构：
[Liang, Yi-Zeng] C;Cent S Univ, Coll Chem & Chem Engn, Res Ctr Modernizat Chinese Med, Changsha 410083, Peoples R China.
关键词：
Characteristic fragmentation mechanism;Initial ionization site;Radical site;Atomic spin density;Ionization energy
摘要：
The characteristic fragmentation mechanisms of the derivatives of tryptophan and tryptamine are investigated by the density functional theory (DFT) at the B3LYP/6-31+G (d, p) level. The main primary alpha-cleavage fragmentation has been predicted through determining the initial ionization site by calculated spin density of molecular radical cation and the variation of molecular structures from neutral to cationic form. The results show that the more intensive base peak produced by main primary alpha-cleavage fragmentation predicted in the present agrees well with those of experimental mass spectra, which is useful for characterizing the fragmentation mechanisms of such indole alkaloids. More importantly, the novel method for determining the most likely initial ionization site proposed in this study is prior to that based on the ionization energy (IE). It will play an important role in unraveling the mechanisms of radical-driven fragmentation not only for small molecules, but also for gas-phase peptides occurring in electron capture dissociation (ECD), electron transfer dissociation (ETD), or low-energy CID of peptide radical cations. However, this study is inappropriate for negative systems. (C) 2009 Elsevier B.V. All rights reserved.
关键词：
drug delivery;biocompatibility;titania nanotubes;cell adhesion;cisplatin
摘要：
TiO2 nanotube (NT) arrays have been prepared by anodic oxidation of a Ti sheet, and carbon-deposited TiO2 NT arrays have been prepared by annealing TiO2 NT arrays in carbon atmosphere. The biocompatibility of the as-prepared NT arrays was investigated by observing the growth of osteosarcoma (MG-63) cells on the NT arrays. The application of the TiO2 NT arrays as a drug delivery vehicle was investigated. Both the TiO2 NTs and the carbon-modified TiO2 NTs have good biocompatibility supporting the normal growth and adhesion of MG-63 cells with no need of extracellular matrix protein coating. The one end-opened TiO2 NTs can be easily filled with drugs, working as an efficient drug delivery vehicle.
摘要：
Comparison of the volatile constituents of different parts of Cortex magnolia officinalis by GC-MS combined with chemometric resolution method Volatile compositions of different parts (stem, branch and root barks) of Cortex magnolia officinalis, cultivated in China, were investigated for the first time by GC-MS with the help of heuristic evolving latent projection (HELP). Identification of components was conducted by similarity matching to NIST mass library but also assisted by comparison of temperature-programmed retention indices (PTRIs) with the data web available. A total of 90, 82 and 76 volatile compounds in the essential oils of the three samples taken from the same batch aforementioned were qualitatively and quantitatively determined, representing 84.03, 83.68 and 83.10% of the total content, respectively. Among the constituents determined, there were 50 components coexisting. Eudesmol and its isomers were shown to be the principal compounds in the studied samples, accounting for 47.66, 36.74 and 36.31%, respectively. The three kinds of isomers (a-, band c-eudesmol) in houpo volatile oils have been tentatively qualified and quantified simultaneously for the first time. By comparative analysis, significant qualitative and semi-quantitative differences and similarities were observed among the three samples. The results achieved provide a scientific evidence for further exploitation of Magnolia bark and clinical medication.
摘要：
Evidences indicate that a complex relationship exists among sleep disorders, obesity and insulin resistance. NEU-P11 is a novel melatonin agonist used in treatment of psychophysiological insomnia, and in animal studies NEU-P11 showed sleep-promoting effect. In this study, we applied NEU-P11 on obese rats to assess its potential melatoninergic effects in vivo. Obese models were established using high-fat/high-sucrose-fed for 5 months. NEU-P11 (10 mg/kg)/melatonin (4 mg/kg)/vehicle were administered by a daily intraperitoneal injection respectively for 8 weeks. Our results showed that NEU-P11 or melatonin inhibited both body weight gain and deposit of abdominal fat with no influence on food intake. The impaired insulin sensitivity and antioxidative potency were improved and the levels of plasma glucose, total cholesterol (TC), triglycerides (TG) decreased with an increased in HDL-cholesterol (HDL-c) after NEU-P11 or melatonin administration. These data suggest that NEU-P11, like melatonin, decreased body weight gain and improved insulin sensitivity and metabolic profiles in obese rats. We conclude that NEU-P11 has a melatoninergic effect on regulating body weight in obese rats and also improving metabolic profiles and efficiently enhancing insulin sensitivity. (C) 2009 Elsevier Ltd. All rights reserved.
期刊：
Journal of Heterocyclic Chemistry,2009年46(2):256-260 ISSN：0022-152X
通讯作者：
Wang, Hong-Qing
作者机构：
[Wang, Yu-Yuan; Wang, Hong-Qing; Lin, Can-Rong] Univ S China, Coll Chem & Chem Engn, Hunan 421001, Peoples R China.;[Zhou, Wei-Ping] Univ S China, Coll Math & Phys, Hunan 421001, Peoples R China.;[Liu, Zhao-Jie] Cent China Normal Univ, Coll Chem, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China.
通讯机构：
[Wang, Hong-Qing] U;Univ S China, Coll Chem & Chem Engn, Hunan 421001, Peoples R China.
摘要：
A novel approach to regioselective synthesis of new 5‐amino‐6‐arylamino‐1H‐pyrazolo[3,4‐d]pyrimidin‐4(5H)‐one 5 derivatives via a tandem aza‐wittig and annulation reaction of iminophosphorance 2, aromatic isocyanates and hydrazine in 69.6–94.7% isolated yields is reported. The compound 5 reacted with triethyl orthoformate to give compound 6 in good yield (65.8–82.8%). Their structure was clearly confirmed by spectroscopy data (IR, 1H NMR, MS, elemental analysis) and the results of preliminary bioassay indicated that compounds 5 and 6 possess high antifungal activity against Botrytis cinerea Pers and Sclerotinia sclerotiorum, and compound 5h showed 100, 96.4, and 90.2% inhibitory rate to Botrytis cinerea Pers, Pyricularia oryzae, and Sclerotinia sclerotiorum at the concentration of 50 mg/L. The antifungal activities of compound 6 were generally higher than those of compound 5. J. Heterocyclic Chem., (2009).
