期刊：
Lipids in Health and Disease,2011年10(1):1-9 ISSN：1476-511X
通讯作者：
Fu, Mingde
作者机构：
[Tian, Li; Fu, Mingde; Jia, Lianqun] Sichuan Univ, W China Hosp, Lab Endocrinol & Metab, Chengdu 610041, Sichuan, Peoples R China.;[Long, Shiyin; Liu, Yinghui] Univ S China, Dept Biochem & Mol Biol, Hengyang, Hunan, Peoples R China.;[Xu, Yanhua] Chengdu Hoist Biotechnol Co LTD, Chengdu, Sichuan, Peoples R China.
通讯机构：
[Fu, Mingde] S;Sichuan Univ, W China Hosp, Lab Endocrinol & Metab, Chengdu 610041, Sichuan, Peoples R China.
关键词：
Total Cholesterol;Total Cholesterol Level;High Density Lipoprotein;Total Cholesterol Concentration;High Total Cholesterol
摘要：
To investigate alteration of high density lipoproteins (HDL) subclasses distribution in different total cholesterol (TC) levels, mainly the characteristics of HDL subclasses distribution in desirable TC levels and analyze the related mechanisms. ApoA-I contents of plasma HDL subclasses were determined by 2-dimensional gel electrophoresis coupled with immunodetection. 486 Chinese Adults subjects were assigned to different TC groups according to the third Report of NCEP (ATP- III) guidelines. The increase in contents of small preβ1-HDL, HDL3c, HDL3b, and HDL3a particles clustered and reduce in HDL2b with increased of TC. The distribution of HDL subclasses have shown abnormality characterized by the lower HDL2b (324.2 mg/L) contents and the higher preβ1-HDL (90.4 mg/L) contents for desirable TC Chinese subjects. Among 176 desirable TC subjects, 58.6% subjects with triglyceride (TG) < 2.26 mmol/L, 61.2% subjects with HDL-C ≥1.03 mmol/L and 88.6% subjects with low density lipoprotein cholesterol(LDL-C) < 3.34 mmol/L, and the profile of HDL subclasses distribution for above these subjects was reasonable. The particles size of HDL subclasses shifted towards smaller with increased TC levels. The TC was liner with HDL2b contents and those can be reduced 17 mg/L for 0.5 mmol/L increment in TC levels. The HDL subclasses distribution phenotype was not expectation for Chinese Population with desirable TC levels. Thus, from the HDL subclasses distribution point, when assessing the coronary heart disease(CHD) risk not only rely on the TC levels, but also the concentrations of TG, HDL-C and LDL-C must considered in case the potential risk for desirable TC subjects with other plasma lipids metabolism disorders.
期刊：
JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY,2011年11(3):2389-2397 ISSN：1533-4880
通讯作者：
Chen, Chunying
作者机构：
[Liu, Ying; Du, Jiangfeng; Li, Wei; Chen, Chunying; Ge, Cuicui; Li, Yufeng; Qiu, Yang] Inst High Energy Phys, CAS Key Lab Biol Effects Nanomat & Nanosafety, Beijing 100190, Peoples R China.;[Liu, Ying; Du, Jiangfeng; Li, Wei; Chen, Chunying; Ge, Cuicui; Li, Yufeng; Qiu, Yang] Natl Ctr Nanosci & Technol China, Beijing 100190, Peoples R China.;[Gao, Yuxi; Li, Wei; Chai, Zhifang; Chen, Chunying; Li, Bai; Ge, Cuicui; Li, Yufeng] Chinese Acad Sci, Inst High Energy Phys, CAS Key Lab Nucl Analyt Tech, Beijing 100049, Peoples R China.;[Du, Jiangfeng] Univ S China, Sch Chem & Chem Engn, Hengyang 421001, Peoples R China.
通讯机构：
[Chen, Chunying] I;Inst High Energy Phys, CAS Key Lab Biol Effects Nanomat & Nanosafety, Beijing 100190, Peoples R China.
关键词：
Catalyst Elements;Carbon Nanotubes;Inductively Coupled Plasma Mass Spectrometry;Neutron Activation Analysis
摘要：
Commercially available carbon nanotubes (CNT) often contain some quantities of metallic and carbonaceous impurities. These impurities influence their physicochemical properties and performance, and accordingly a number of potential applications. The lack of information of metal impurities may also preclude accurate environmental and health risk assessments for specific CNT materials. To address these needs, a quantitative analysis of the metal contents has been made in a number of commercial carbon nanotubes produced by different manufacturers. More than 20 metals or metalloids were determined by neutron activation analysis. The results indicate arranging from 0.44 to 3 wt% of catalyst residues remained although the producers claim to provide a catalyst-free product. Most of the impurity elements are transition metals, such as Fe, Ni, Mo, Y, Co and Cr. In addition to the expected catalyst residues, other unexpected impurity elements were detected including As, Gd, W, Yb, Sm and so on. Metallic impurities in carbon nanotube materials should come from the large-scale production procedures, post fabrication and post-purification treatments. The analytical results determined by inductively-coupled plasma mass spectrometry show that a further deep purification using conventional acid reflux cannot completely remove the metallic impurities from carbon nanotubes. Post-production clean up is difficult and often incompletely.
期刊：
Lipids in Health and Disease,2010年9(1):1-10 ISSN：1476-511X
通讯作者：
Qin, Yang
作者机构：
[Qin, Yang; Tian, Li; Liu, Yinghui] Sichuan Univ, Dept Biochem & Mol Biol, W China Sch Preclin & Forens Med, Chengdu 610041, Sichuan, Peoples R China.;[Fu, Mingde] Sichuan Univ, Lab Endocrinol & Metab, W China Hosp, Chengdu 610041, Sichuan, Peoples R China.;[Long, Shiyin] Univ S China, Dept Biochem & Mol Biol, Hengyang 421001, Peoples R China.;[Xu, Yanhua] Chengdu Hoist Biotechnol Co LTD, Chengdu 610075, Peoples R China.
通讯机构：
[Qin, Yang] S;Sichuan Univ, Dept Biochem & Mol Biol, W China Sch Preclin & Forens Med, Chengdu 610041, Sichuan, Peoples R China.
关键词：
Reverse Cholesterol Transport;Cholesterol Ester Transfer Protein;Quebec Cardiovascular Study;West China Medical;Monitor Atherosclerosis Regression Study
摘要：
To evaluate the relationship between the low-density lipoprotein cholesterol (LDL-C)/high-density lipoprotein cholesterol (HDL-C) ratio and HDL subclass distribution and to further examine and discuss the potential impact of LDL-C and HDL-C together with TG on HDL subclass metabolism. Small-sized preβ1-HDL, HDL3b and HDL3a increased significantly while large-sized HDL2a and HDL2b decreased significantly as the LDL-C/HDL-C ratio increased. The subjects in low HDL-C level (< 1.03 mmol/L) who had an elevation of the LDL-C/HDL-C ratio and a reduction of HDL2b/preβ1-HDL regardless of an undesirable or high LDL-C level. At desirable LDL-C levels (< 3.34 mmol/L), the HDL2b/preβ1-HDL ratio was 5.4 for the subjects with a high HDL-C concentration (≥ 1.55 mmol/L); however, at high LDL-C levels (≥ 3.36 mmol/L), the ratio of LDL-C/HDL-C was 2.8 in subjects, and an extremely low HDL2b/preβ1-HDL value although with high HDL-C concentration. With increase of the LDL-C/HDL-C ratio, there was a general shift toward smaller-sized HDL particles, which implied that the maturation process of HDL was blocked. High HDL-C concentrations can regulate the HDL subclass distribution at desirable and borderline LDL-C levels but cannot counteract the influence of high LDL-C levels on HDL subclass distribution.
摘要：
A new spectrophotometric method for the determination of nicotine in mixtures without pre-separation has been proposed. Nicotine could react with 2,4-dinitrophenol through a charge-transfer reaction to form a colored complex. The second-order data from the visible absorption spectra of the complex in a series of ethanol–water binary solvents with various water volume fractions could be expressed as the combination of two bilinear data matrices. With the bilinear model, the second-order spectra data of mixtures containing nicotine and other interferents could be analysed by using second-order calibration algorithms, and the determination of nicotine in the mixtures could be achieved. The algorithm used here was parallel factor analysis. The method has been successfully used to determine nicotine in tobacco samples with satisfactory results.
摘要：
Forkhead box protein A1 (FoxA1) is an evolutionarily conserved winged helix transcription factor with diverse regulatory functions. However, little is known about the role of FoxA1 in acute lung injury (ALI) and pulmonary cell injury. In this study, an in vivo model was employed whereby rats were administered an intravenous injection of oleic acid (OA, 0.1 ml/kg), and alveolar type II epithelial cells (AT-2 cells) injury was induced by hydrogen peroxide (H2O2) in vitro. OA injection resulted in lung injury and AT-2 cells apoptosis in vivo. OA injection and H2O2 upregulated FoxA1 mRNA and protein in lung tissue of the in vivo ALI model and in H2O2 challenged AT-2 cells. Overexpression of FoxA1 promoted apoptosis, whereas FoxA1 deficiency, induced by antisense oligonucleotides, decreased AT-2 cells apoptosis induced by H2O2, as shown by flow cytometry. These results suggest that FoxA1 may play an important role in ALI by promoting apoptosis of pulmonary epithelial cells.
摘要：
Comparison of the volatile constituents of different parts of Cortex magnolia officinalis by GC-MS combined with chemometric resolution method Volatile compositions of different parts (stem, branch and root barks) of Cortex magnolia officinalis, cultivated in China, were investigated for the first time by GC-MS with the help of heuristic evolving latent projection (HELP). Identification of components was conducted by similarity matching to NIST mass library but also assisted by comparison of temperature-programmed retention indices (PTRIs) with the data web available. A total of 90, 82 and 76 volatile compounds in the essential oils of the three samples taken from the same batch aforementioned were qualitatively and quantitatively determined, representing 84.03, 83.68 and 83.10% of the total content, respectively. Among the constituents determined, there were 50 components coexisting. Eudesmol and its isomers were shown to be the principal compounds in the studied samples, accounting for 47.66, 36.74 and 36.31%, respectively. The three kinds of isomers (a-, band c-eudesmol) in houpo volatile oils have been tentatively qualified and quantified simultaneously for the first time. By comparative analysis, significant qualitative and semi-quantitative differences and similarities were observed among the three samples. The results achieved provide a scientific evidence for further exploitation of Magnolia bark and clinical medication.
摘要：
Evidences indicate that a complex relationship exists among sleep disorders, obesity and insulin resistance. NEU-P11 is a novel melatonin agonist used in treatment of psychophysiological insomnia, and in animal studies NEU-P11 showed sleep-promoting effect. In this study, we applied NEU-P11 on obese rats to assess its potential melatoninergic effects in vivo. Obese models were established using high-fat/high-sucrose-fed for 5 months. NEU-P11 (10 mg/kg)/melatonin (4 mg/kg)/vehicle were administered by a daily intraperitoneal injection respectively for 8 weeks. Our results showed that NEU-P11 or melatonin inhibited both body weight gain and deposit of abdominal fat with no influence on food intake. The impaired insulin sensitivity and antioxidative potency were improved and the levels of plasma glucose, total cholesterol (TC), triglycerides (TG) decreased with an increased in HDL-cholesterol (HDL-c) after NEU-P11 or melatonin administration. These data suggest that NEU-P11, like melatonin, decreased body weight gain and improved insulin sensitivity and metabolic profiles in obese rats. We conclude that NEU-P11 has a melatoninergic effect on regulating body weight in obese rats and also improving metabolic profiles and efficiently enhancing insulin sensitivity. (C) 2009 Elsevier Ltd. All rights reserved.
摘要：
Apocytochrome b 5 (apocyt b 5), a small b-type cytochrome with heme prosthetic group removal, has been subjected to steered molecular dynamics (SMD) simulations for investigating the consequences of mechanical force-induced unfolding. Both constant velocity (0.5 and 1.0 Å/ps) and constant force (500, 750 and 1000 pN) stretching have been employed to model forced unfolding of apocyt b 5. The results of SMD simulations elucidate that apocyt b 5 is protected against external stress mainly through the interstrand hydrogen bonding between its β1–β2 and β2–β3 strands, highlighting the importance of hydrophobic core 2 in stabilization of apocyt b 5. The existence of intermediate states manifested by current simulations in the forced unfolding pathway of apocyt b 5 is different from the observations in pervious thermal or chemical unfolding studies in the absence of force. The present study could thus provide insights into the relationship between the two cooperative functional modules of apocyt b 5 and also guide the rational molecular design of heme proteins.
