期刊：
Science of The Total Environment,2024年917:170369 ISSN：0048-9697
通讯作者：
Yuanyuan Gao<&wdkj&>Yayi Wang
作者机构：
[Zhou, Shuai; Huang, Zefeng; Yang, Fengjuan] Hunan Province Key Laboratory of Pollution Control and Resources Reuse Technology, School of Civil Engineering, University of South China, Hengyang 421001, China;[Zhou, Shuai] Hunan Province Key Laboratory of Rare Metal Minerals Exploitation and Geological Disposal of Wastes, School of Resources Environment and Safety Engineering, University of South China, Hengyang 421001, China;[Wang, Weigang] State Key Laboratory of Pollution Control and Resources Reuse, College of Environmental Science and Engineering, Tongji University, Siping Road, Shanghai 200092, China;[Yang, Zhengqing] College of Environmental Science and Engineering, Hunan University, Changsha 410082, China;[Jiang, Tianyun; Song, Jian] Hunan Province Key Laboratory of Rare Metal Minerals Exploitation and Geological Disposal of Wastes, School of Resources Environment and Safety Engineering, University of South China, Hengyang 421001, China
通讯机构：
[Yuanyuan Gao] H;[Yayi Wang] S;Hunan Province Key Laboratory of Rare Metal Minerals Exploitation and Geological Disposal of Wastes, School of Resources Environment and Safety Engineering, University of South China, Hengyang 421001, China<&wdkj&>State Key Laboratory of Pollution Control and Resources Reuse, College of Environmental Science and Engineering, Tongji University, Siping Road, Shanghai 200092, China
摘要：
The emergence of antibiotic resistance genes (ARGs) and antibiotic-resistant bacteria (ARB) in the environment is well established as a human health crisis. The impact of radioactive heavy metals on ecosystems and ultimately on human health has become a global issue, especially for the regions suffering various nuclear activities or accidents. However, whether the radionuclides can affect the fate of antibiotic resistance in bacteria remains poorly understood. Here, the dynamics of ARB, three forms of ARGs-intracellular ARGs (iARGs), adsorbed extracellular ARGs (aeARGs), and free extracellular ARGs (feARGs)-and microbial communities were investigated following exposure to uranium (U), a representative radioactive heavy metal. The results showed that 90-d of U exposure at environmentally relevant concentrations of 0.05mg/L or 5mg/L significantly increased the ARB concentration in activated sludge (p<0.05). Furthermore, 90-d of U exposure slightly elevated the absolute abundance of aeARGs (except tetO) and sulfonamide iARGs, but decreased tetracycline iARGs. Regarding feARGs, the abundance of tetC, tetO, and sul1 decreased after 90-d of U stress, whereas sul2 showed the opposite trend. Partial least-squares path model analysis revealed that the abundance of aeARGs and iARGs under U stress was predominantly driven by increased cell membrane permeability/intI1 abundance and cell membrane permeability/reactive oxygen species concentration, respectively. Conversely, the changes in feARGs abundance depended on the composition of the microbial community and the expression of efflux pumps. Our findings shed light on the variations of ARGs and ARB in activated sludge under U exposure, providing a more comprehensive understanding of antibiotic resistance risks aggravated by radioactive heavy metal-containing wastewater.
摘要：
To investigate the strengthening effects and mechanisms of bioaugmentation on the microbial remediation of uranium-contaminated groundwater via bioreduction coupled to biomineralization, two exogenous microbial consortia with reducing and phosphate-solubilizing functions were screened and added to uranium-contaminated groundwater as the experimental groups (group B, reducing consortium added; group C, phosphate-solubilizing consortium added). β-glycerophosphate (GP) was selected to stimulate the microbial community as the sole electron donor and phosphorus source. The results showed that bioaugmentation accelerated the consumption of GP and the proliferation of key functional microbes in groups B and C. In group B, Dysgonomonas, Clostridium_sensu_stricto_11 and Clostridium_sensu_stricto_13 were the main reducing bacteria, and Paenibacillus was the main phosphate-solubilizing bacteria. In group C, the microorganisms that solubilized phosphate were mainly unclassified_f_Enterobacteriaceae. Additionally, bioaugmentation promoted the formation of unattached precipitates and alleviated the inhibitory effect of cell surface precipitation on microbial metabolism. As a result, the formation rate of U-phosphate precipitates and the removal rates of aqueous U(VI) in both groups B and C were elevated significantly after bioaugmentation. The U(VI) removal rate was poor in the control group (group A, with only an indigenous consortium). Propionispora, Sporomusa and Clostridium_sensu_stricto_11 may have played an important role in the removal of uranium in group A. Furthermore, the addition of a reducing consortium promoted the reduction of U(VI) to U(IV), and immobilized uranium existed in the form of U(IV)-phosphate and U(VI)-phosphate precipitates in group B. In contrast, U was present mainly as U(VI)-phosphate precipitates in groups A and C. Overall, bioaugmentation with an exogenous consortium resulted in the rapid removal of uranium from groundwater and the formation of U-phosphate minerals and served as an effective strategy for improving the treatment of uranium-contaminated groundwater in situ.
摘要：
Levels of cadmium (Cd) and lead (Pb) correspond to common composition in acid mine wastewater of Hunan Province of China. The removal path of Cd and Pb and the structure of microbial community were investigated by developing constructed wetlands (CWs) with different layer positions of biochar. The biochar as a layer at the bottom of CW (BCW) system exhibited maximum Cd and Pb removal efficiencies of 96.6-98.6% and 97.2-98.9%, respectively. Compared with original soil, BCW increased the relative proportions of Proteobacteria, Firmicutes, Acidobacteriota, Verrucomicrobiota, Desulfobacterota, Armatimonadota, Bacteroidota, Patescibacteria, Basidiomycota (phylum level) and Burkholderia-Caballeronia-Paraburkholderia, Citrifermentans, Chthonomonadales, Cellulomonas, Geothrix, Terracidiphilus, Gallionellaceae, Microbacterium, Vanrija, Apiotrichum, Saitozyma, Fusarium (genus level). The concentrations of Cd and Pb were positively correlated with the abundance of Verrucomicrobiota, Basidiomycota (phylum level), and Methylacidiphilaceae, Meyerozyma, Vanrija (genus level). This study demonstrates that BCW system can improve removal performance toward Cd and Pb, as well as alter microbial community.
摘要：
The reduction of soluble U(VI) to insoluble and less toxic U(IV) by photocatalysis is an effective method to control uranium contamination. The graphitic carbon nitride nanosheet (CNN)/UiO-66 composites (CNNU) were prepared by thermal polymerization and solvothermal methods for the removal of U(VI). The morphology, crystal structure and optical properties of composites were analyzed by SEM, XRD, BET, UV-DRS, PL and EIS. The results showed the introduction of UiO-66 increased the specific surface of CNN from 9.07 m(2)/g to 46.24 m(2)/g, and effectively suppressed the recombination of photogenerated electrons and holes and improved the photocatalytic activity. The U(VI) removal capacity by adsorption and photocatalysis of CNNU was reached 779.47mg/g, which significantly higher than that of adsorption (478.38mg/g). The adsorption process was found to conform to the pseudo-second-order kinetic model and the Langmuir isothermal model. Meanwhile, U(VI) adsorbed on the CNNU was reduced to U(IV) via e(-) and ·O(2)(-) generated in the photocatalytic process. Therefore, this outstanding performance of CNNU in U(VI) removal is attributed to the synergistic effect of adsorption and photocatalytic reduction.
作者机构：
[Zhou, Shuai; Wang, Yayi; Zhang, Yalei] Tongji Univ, Coll Environm Sci & Engn, State Key Lab Pollut Control & Resources Reuse, Siping Rd, Shanghai 200092, Peoples R China.;[Zhou, Shuai; Zhang, Siqi; Duan, Yi; Yang, Zhengqing] Univ South China, Sch Civil Engn, Hunan Prov Key Lab Pollut Control & Resources Reus, Hengyang 421001, Peoples R China.;[Gao, Yuanyuan; Tang, Zhenping] Univ South China, Hunan Prov Key Lab Rare Met Minerals Exploitat & G, Hengyang 421001, Peoples R China.
通讯机构：
[Wang, YY ] T;Tongji Univ, Coll Environm Sci & Engn, State Key Lab Pollut Control & Resources Reuse, Siping Rd, Shanghai 200092, Peoples R China.
关键词：
Activated sludge;Environmental condition;Extracellular antibiotic resistance genes;Metagenomics;Starvation;Substrate type
摘要：
Extracellular antibiotic resistance genes (eARGs) are important emerging environmental pollutants in wastewater treatment plants (WWTPs). Nutritional substrate deficiency (i.e., starvation) frequently occurs in WWTPs owing to annual maintenance, water quality fluctuation, and sludge storage; and it can greatly alter the antibiotic resistance and extracellular DNA content of bacteria. However, the fate and corresponding transmission risk of eARGs in activated sludge under starvation stress remain largely unknown. Herein, we used metagenomic sequencing to explore the effects of starvation scenarios (carbon, nitrogen, and/or phosphorus deficiency) and environmental conditions (alternating anaerobic-aerobic, anaerobic, anoxic, and aerobic) on the distribution, mobility, and hosts of eARGs in activated sludge. The results showed that 30 days of starvation reduced the absolute abundances of eARGs by 40.9%-88.2%, but high-risk dual and multidrug resistance genes persisted. Starvation, particularly the simultaneous lack of carbon, nitrogen, and phosphorus under aerobic conditions, effectively alleviated eARGs by reducing the abundance of extracellular mobile genetic elements (eMGEs). Starvation also altered the profile of bacterial hosts of eARGs and the bacterial community composition, the latter of which had an indirect positive effect on eARGs via changing eMGEs. Our findings shed light on the response patterns and mechanisms of eARGs in activated sludge under starvation conditions and highlight starvation as a potential strategy to mitigate the risk of previously neglected eARGs in WWTPs.
摘要：
Phosphorus-rich biochar (PBC) has been extensively studied due to its significant adsorption effect on U(VI). However, the release of phosphorus from PBC into solution decreases its adsorption performance and reusability and causes phosphorus pollution of water. In this study, Alcaligenes faecalis (A. faecalis) was loaded on PBC to produce a novel biocomposite (A/PBC). After adsorption equilibrium, phosphorus released into solution from PBC was 2.32mg/L, while it decreased to 0.34mg/L from A/PBC (p<0.05). The U(VI) removal ratio of A/PBC reached nearly 100%, which is 13.08% higher than that of PBC (p<0.05), and it decreased only by 1.98% after 5 cycles. When preparing A/PBC, A. faecalis converted soluble phosphate into insoluble metaphosphate minerals and extracellular polymeric substances (EPS). And A. faecalis cells accumulated through these metabolites and formed biofilm attached to the PBC surface. The adsorption of metal cations on phosphate further contributed to phosphorus fixation in the biofilm. During U(VI) adsorption by A/PBC, A. faecalis synthesize EPS and metaphosphate minerals by using the internal components of PBC, thus increasing the abundance of acidic functional groups and promoting U(VI) adsorption. Hence, A/PBC can be a green and sustainable material for U(VI) removal from wastewater.
摘要：
Electrocatalytic oxidation is commonly restricted by low degradation efficiency, slow mass transfer, and high energy consumption. Herein, a synergetic electrocatalysis system was developed for removal of various drugs, i.e., atenolol, florfenicol, and diclofenac sodium, as well as actual pharmaceutical wastewater, where the newly-designed single-atom Zr embedded Ti(4)O(7) (Zr/Ti(4)O(7)) and hierarchical CuFe(2)O(4) (CFO) microspheres were used as anode and microelectrodes, respectively. In the optimal reaction system, the degradation efficiencies of 40mg L(-1) atenolol, florfenicol, and diclofenac sodium could achieve up to 98.8%, 93.4%, and 85.5% in 120min with 0.1g L(-1) CFO at current density of 25mA cm(-2). More importantly, in the flow-through reactor, the electrooxidation lasting for 150min could reduce the COD of actual pharmaceutical wastewater from 432 to 88.6mg L(-1), with a lower energy consumption (25.67kWh/m(3)). Meanwhile, the electrooxidation system maintained superior stability and environmental adaptability. DFT theory calculations revealed that the excellent performance of this electrooxidation system could be ascribed to the striking features of the reduced reaction energy barrier by single-atom Zr loading and abundant oxygen vacancies on the Zr/Ti(4)O(7) surface. Moreover, the characterization and experimental results demonstrated that the CFO unique hierarchical structure and synergistic effect between electrodes were also the important factors that could improve the system performance. The findings shed light on the single-atom material design for boosting electrochemical oxidation performance.
通讯机构：
[Taotao Zeng] H;Hunan Province Key Laboratory of Pollution Control and Resources Reuse Technology, University of South China, Hengyang, 421001, Hunan, China
摘要：
The use of modified biochar for the remediation of heavy metal (HM) has received much attention. However, the immobilization mechanism of biochar to multiple HMs and the interaction of different forms of HMs with microorganisms are still unclear. K(2)HPO(4)-modified biochar (PBC) was produced and used in a 90-days immobilization experiment with soil collected from a typic lead-zinc (Pb-Zn) mining soil. Incubation experiments showed that PBC enhanced the transformation of Cd, Pb, Zn and Cu from exchangeable (Ex-) and/or carbonate-bound forms (Car-) to organic matter-bound (Or-) and/or residual forms (Re-). After scanning electron microscopy-energy dispersive X-ray spectrometer (SEM-EDS), X-ray diffractometry (XRD), fourier transform infrared (FTIR), thermogravimetric analysis (TGA) and X-ray photoelectron spectroscopy (XPS) analysis, the mechanisms of HM immobilization by PBC were proposed as precipitation (PO(4)(3-), HPO(4)(2-), OH(-) and CO(3)(2-)), electrostatic attraction, complexation (-COOH, -OH and R-O-H) and the indirect roles of soil parameter variations (pH, moisture and microbial community). Microbial community analysis through high-throughput sequencing showed that PBC reduced bacterial and fungal abundance. However, addition of PBC increased the relative proportions of Proteobacteria by 15.04%-42.99%, Actinobacteria by 4.74%-22.04%, Firmicutes by 0.76%-23.35%, Bacteroidota by 0.16%-12.34%, Mortierellomycota by 4.00%-9.66% and Chytridiomycota by 0.10%-13.7%. Ex-Cd/Pb/Zn, Car-Cd/Zn and Re-Cd/Pb/As were significantly positively (0.001<P≤0.05) correlated with bacterial phyla of Crenarchaeota and Methylomirabilota, and Re-Cu and Ex-/Car-/Fe-Mn oxide-bound (Fe-Mn-)/Or-As were significantly positively correlated (0.001<P≤0.05) with the bacterial phyla of Proteobacteria and Bacteroidota. While Car-Cd/Zn and Re-Pb/As were positively correlated (0.01<P≤0.05) with fungal phyla of Ascomycota, Glomeromycota, Kickxellomycota, Basidiomycota and Mucoromycota. The bacterial network contained more complex interactions than the fungal network, suggesting that bacteria play a larger role in HMs transformation processes. The results indicate that PBC is an effective agent for the remediation of HMs polluted soil in Pb-Zn mining areas.
摘要：
The simultaneous electro-oxidation of Ni (II)-citrate and electrodeposition recovery of nickel metal were attempted in a combined electro-oxidation-electrodeposition reactor with a boron-doped diamond (BDD) anode and a polished titanium cathode. Effects of initial nickel citrate concentration, current density, initial pH, electrode spacing, electrolyte type, and initial electrolyte dosage on electrochemical performance were examined. The efficiencies of Ni (II)-citrate removal and nickel metal recovery were determined to be 100% and over 72%, respectively, under the optimized conditions (10 mA/cm(2), pH 4.09, 80 mmol/L Na2SO4, initial Ni (II)-citrate concentration of 75 mg/L, electrode spacing of 1 cm, and 180 min of electrolysis). Energy consumption increased with increased current density, and the energy consumption was 0.032 kWh/L at a current density of 10 mA/cm(2) (pH 6.58). The deposits at the cathode were characterized by scanning electron microscopy (SEM), energy-dispersive spectrometry (EDS), X-ray diffraction (XRD), and X-ray photoelectron spectroscopy (XPS). These characterization results indicated that the purity of metallic nickel in cathodic deposition was over 95%. The electrochemical system exhibited a prospective approach to oxidize metal complexes and recover metallic nickel. (C) 2022 The Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences. Published by Elsevier B.V.
作者机构：
[Yin, Xiangdong; Xiao, Xiao; Dai, Naxin; Wang, Yan] School of Civil Engineering, University of South China, Hengyang, Hunan Province, China;[Xie, Lei] Centre for Future Materials, University of Southern Queensland,Toowoomba, Australia
摘要：
Nowadays there are many types of glass fiber reinforced polymer(GFRP) composite beam and column joints, such as standard connection, bolted through connection, angle steel connection, tube connection and so on, most of which connected by high-strength bolts with round holes. In this paper, monotonic loading tests on GFRPcomposite beam and column joints connected by slotted-hole bolts were conducted. To compare the performance of different joints, two groups of specimens were used in this study; one of group was the beam-column joints connected by the angle steel, and other group was connected by the tube connection. Specimens with different bolt holes, side plate reinforcement condition, and different bolt pre-tightening forces were studied. Failure modes, bending moment curves, plastic rotation, and yield stiffness of the two groups of joints were compared. Results showed thatthe ultimate bending moment bearing capacity of specimens with side plates could be increased by 30%. Under the same conditions, the bearing capacity of the tube joints was about 10% larger than that of the angle steel joints. Although the bearing capacity of joints was not increased by using slotted holes, plastic rotation capacity and yield stiffness of joints with slotted-hole bolts were 1.1 times than that of the ordinary round-hole bolts joints.
作者机构：
[Zhang, Xiaoling; Wang, Liangqin; Liu, Yingjiu; Liu, Jinxiang; Zeng, Taotao; Wang, Guohua] Univ South China, Hunan Prov Key Lab Pollut Control & Resources Reu, Hengyang 421001, Peoples R China.;[Wang, Aijie] Chinese Acad Sci, Key Lab Environm Biotechnol, Beijing 100085, Peoples R China.
通讯机构：
[Taotao Zeng] H;Hunan Province Key Laboratory of Pollution Control and Resources Reuse Technology, University of South China, Hengyang, 421001, China
摘要：
In this study, a complex bacterial consortium was enriched from a typical Pb-Zn mine area and immobilized by sodium alginate to form biospheres, which were used for treatment of selenite (Se(IV))- and cadmium (Cd(II))-containing wastewater without external carbon source. Batch experiments showed that the maximum Se(IV) removal efficiency was 92.36% under the optimal conditions of an initial pH of 5, dosage of 5g/L, initial Se(IV) concentration of 7.9mg/L and reaction time of 168h. Subsequently, more than 99% of 11.2mg/L Cd(II) was removed by the biospheres within 10h. Physicochemical characterization showed that reduction and adsorption were the main mechanisms for Se(IV) and Cd(II) removal, respectively. During the removal process, selenium and CdSe nanoparticles were formed. Bacterial community analysis showed the dominant bacterial genera changed after treatment of Se(IV)- and Cd(II)-containing wastewater. Additionally, 16S rRNA gene function prediction results showed that amino acid transport, carbohydrate transport, ion transport and metabolism were the dominant gene functions. The present study provides a potential way for the biological treatment of Se(IV)- and Cd(II)-containing wastewater using immobilized biospheres without external carbon source in short-term.
期刊：
Science of The Total Environment,2022年840:156594 ISSN：0048-9697
通讯作者：
Shuai Zhou
作者机构：
[Zhou, Shuai; Duan, Yi; Zeng, Taotao; Tang, Zhenping] Univ South China, Hunan Prov Key Lab Pollut Control & Resources Reus, Hengyang 421001, Peoples R China.;[Gao, Yuanyuan; Zhou, Shuai; Tang, Zhenping] Univ South China, Hunan Prov Key Lab Rare Met Minerals Exploitat & G, Hengyang 421001, Peoples R China.;[Zhou, Shuai; Duan, Yi; Zhang, Siqi; Zhang, Yu; Zeng, Taotao] Univ South China, Sch Civil Engn, Hengyang 421001, Peoples R China.
通讯机构：
[Shuai Zhou] H;Hunan Province Key Laboratory of Pollution Control and Resources Reuse Technology, University of South China, Hengyang 421001, China<&wdkj&>Hunan Province Key Laboratory of Rare Metal Minerals Exploitation and Geological Disposal of Wastes, University of South China, Hengyang 421001, China<&wdkj&>School of Civil Engineering, University of South China, Hengyang 421001, China
摘要：
The activated sludge represents a huge reservoir for antibiotic resistant bacteria (ARB) and antibiotic resistance genes (ARGs). Owing to the wastewater fluctuation, annual maintenance and storage requirement, the activated sludge in wastewater treatment plants (WWTPs) may suffer from substrate deficiency (i.e., starvation). Whereas the starvation has been confirmed to regulate the antibiotic resistance in numerous pure bacteria, its impacts on the antibiotic resistance in activated sludge remain unclear. Here, the dynamics of sulfonamide and tetracycline ARB and corresponding ARGs in three forms including intracellular ARGs (iARGs), adsorbed extracellular ARGs (aeARGs) and free extracellular ARGs (feARGs) in activated sludge upon exposure to starvation were investigated. The results showed that, among the different electron donors (i.e., carbon, nitrogen and phosphate), carbon starvation could effectively reduce the absolute abundance of ARB and aeARGs by up to 1.68 lgs and 2.62 lgs, respectively, and released a small amount of feARGs in wastewater with the maximum value of 1.1 × 10(5) copies/mL due to the high degree of sludge cell lysis and DNA adsorption/degradation. For the different acceptor conditions (that is, alternating anaerobic-aerobic, anaerobic, anoxic and aerobic), the anaerobic-aerobic starvation obviously mitigated the absolute abundance of ARB, aeARGs and iARGs by 0.71 lgs, 3.41 lgs and 1.35 lgs, respectively, via the substantial sludge cell lysis and DNA degradation. These findings demonstrated the response patterns and mechanisms of bacterial resistance in activated sludge to starvation stress, and thus provide clues to control the risk of antibiotic resistance in WWTPs by the starvation strategy.
通讯机构：
[Lu, Y.; Tang, L.] C;College of Environmental Science and Engineering, Hunan University, Changsha, 410082, Hunan, China<&wdkj&>Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha, 410082, Hunan, China
作者机构：
[唐振平; 段毅; 刘迎九; 周帅] Hunan Province Key Laboratory of Pollution Control and Resources Reuse Technology, University of South China, Hengyang;421001, China;Hunan Key Laboratory of Rare Metal Minerals Exploitation and Geological Disposal of Wastes, Hengyang;[肖莎莎; 高媛媛] School of Civil Engineering, University of South China, Hengyang;[吴月月; 陈怡雯] Institute of Pathogenic Biology, Hengyang Medical College, University of South China, Hengyang
关键词：
石化废水;耐药菌(ARB);耐药基因(ARGs);分布;去除;影响因子
摘要：
抗生素耐药性污染已成为全球新兴环境问题之一.本研究选取某座石化废水处理厂,对耐药菌(ARB)和3种形态耐药基因(ARGs):细胞内耐药基因(iARGs) 、细胞外附着态耐药基因(aeARGs)和游离态耐药基因(feARGs)的分布特征与去除效能开展研究.结果表明,废水处理厂中检出四环素、磺胺和氨苄西林这3类ARB,其绝对浓度为8.45×10~2 ~ 2.38×10~5CFU·mL~(-1).厌氧处理可使这3类ARB绝对浓度下降0.04 lg ~ 0.21 lg;曝气和沉淀处理对ARB的影响因其类型而异;出水ARB绝对浓度高出进水水平0.12 lg ~ 0.63 lg.活性污泥中aeARGs和iARGs绝对丰度分别为1.96×10~7 ~ 3.02×10~(10) copies·g~(-1)和5.22×10~7 ~ 4.15×10~(10) copies·g~(-1);而废水中feARGs绝对丰度为5.90×10~8 ~ 1.01×10~(12) copies·L~(-1).厌氧处理可去除0.13 lg ~ 0.65 lg aeARGs和0.04 lg ~ 0.28 lg iARGs;曝气和沉淀处理对aeARGs和iARGs的去除效果受ARGs类型和形态影响;出水中feARGs绝对丰度较进水升高0.06 lg ~ 0.81 lg.冗余分析表明,ARB浓度与COD、Cl~-和总氮浓度显著正相关(P < 0.05); aeARGs丰度与COD和总氮浓度显著正相关(P < 0.05); iARGs和feARGs丰度均与重金属浓度显著正相关(P < 0.05).本研究证实了石化废水处理厂具有ARB和不同形态ARGs的富集风险,并为特种工业废水耐药性污染研究与防治提供理论基础.
作者机构：
[Xie, Shuibo; Gao, Xiang; Zeng, Taotao; Wang, Guohua; Zhang, Shiqi] Univ South China, Hunan Prov Key Lab Pollut Control & Resources Reu, Hengyang, Peoples R China.;[Zeng, Taotao; Lens, Piet N. L.] UNESCO, IHE Inst Water Educ, Delft, Netherlands.;[Xie, Shuibo] Univ South China, Key Discipline Lab Natl Def Biotechnol Uranium Mi, Hengyang, Peoples R China.
通讯机构：
[Zeng, Taotao] U;Univ South China, Hunan Prov Key Lab Pollut Control & Resources Reu, Hengyang, Peoples R China.;UNESCO, IHE Inst Water Educ, Delft, Netherlands.
关键词：
Uranium;Anaerobic granular sludge;Bacterial community composition;High-throughput sequencing
期刊：
Water Science and Technology,2015年71(5):725-733 ISSN：0273-1223
通讯作者：
Li Dong
作者机构：
[Xie Shuibo; Liu Yingjiu; Zeng Taotao] Univ South China, Hunan Prov Key Lab Pollut Control & Resources Reu, Hengyang 421001, Peoples R China.;[Zhang Jie; Zeng Huiping; Li Dong] Beijing Univ Technol, Key Lab Beijing Water Qual Sci & Water Environm R, Beijing 100124, Peoples R China.;[Qiu Wenxin] Beijing Gen Municipal Engn Design & Res Inst Co L, Beijing 100082, Peoples R China.;[Zhang Jie] Harbin Inst Technol, State Key Lab Urban Water Resource & Environm, Harbin 150090, Peoples R China.
通讯机构：
[Li Dong] B;Beijing Univ Technol, Key Lab Beijing Water Qual Sci & Water Environm R, Beijing 100124, Peoples R China.
摘要：
An upflow anaerobic biofilter (AF) was developed to investigate anaerobic ammonium-oxidizing (ANAMMOX) efficiency in treating low-strength wastewater at ambient temperature (15.3-23.2 degrees C). Denaturing gradient gel electrophoresis (DGGE) and fluorescence in situ hybridization were used to investigate treatment effects on the microbial community. Stepwise decreases in influent ammonia concentration could help ANAMMOX bacteria selectively acclimate to low-ammonia conditions. With an influent ammonia concentration of 46.5 mg/L, the AF reactor obtained an average nitrogen removal rate of 2.26 kg/(m(3) day), and a removal efficiency of 75.9%. polymerase chain reaction-DGGE results showed that microbial diversity in the low matrix was greater than in the high matrix. Microbial community structures changed when the influent ammonia concentration decreased. The genus of functional ANAMMOX bacteria was Candidatus Kuenenia stuttgartiensis, which remained stationary across study phases. Visual observation revealed that the relative proportions of ANAMMOX bacteria decreased from 41.6 to 36.3% across three study phases. The AF bioreactor successfully maintained high activity due to the ANAMMOX bacteria adaptation to low temperature and substrate conditions.
摘要：
The adsorption of chromium (VI) ions from aqueous solution by ethylenediamine-modified cross-linked magnetic chitosan resin (EMCMCR) was studied in a batch adsorption system. Chromium (VI) removal is pH dependent and the optimum adsorption was observed at pH 2.0. The adsorption rate was extremely fast and the equilibrium was established within 6-10 min. The adsorption data could be well interpreted by the Langmuir and Temkin model. The maximum adsorption capacities obtained from the Langmuir model are 51.813 mg g(-1), 48.780 mg g(-1) and 45.872 mg g(-1) at 293, 303 and 313 K, respectively. The adsorption process could be described by pseudo-second-order kinetic model. The intraparticle diffusion study revealed that film diffusion might be involved in the present case. Thermodynamic parameters revealed the feasibility, spontaneity and exothermic nature of adsorption. The sorbents were successfully regenerated using 0.1 N NaOH solutions. (C) 2010 Elsevier B.V. All rights reserved.
期刊：
Journal of Environmental Radioactivity,2010年101(6):504-508 ISSN：0265-931X
通讯作者：
Wang, Jing-song
作者机构：
[Wang, Jing-song; Bao, Zheng-lei; Xie, Shui-bo; Hu, Xin-jiang] Univ S China, Sch Urban Construct, Hengyang 421001, Hunan, Peoples R China.;[Wang, Jing-song; Xie, Shui-bo] Univ S China, Hunan Key Lab Pollut Control & Resource Reuse, Henyang 421001, Hunan, Peoples R China.;[Liu, Yun-guo; Hu, Xin-jiang] Hunan Univ, Coll Environm Sci & Engn, Changsha 410082, Hunan, Peoples R China.;[Wang, Jing-song] Univ S China, Sch Urban Construct, 28 W Changsheng Rd, Hengyang 421001, Hunan, Peoples R China.
通讯机构：
[Wang, Jing-song] U;Univ S China, Sch Urban Construct, 28 W Changsheng Rd, Hengyang 421001, Hunan, Peoples R China.
会议名称：
9th International Conference on Biogeochemistry of Trace Elements
会议时间：
JUL, 2007
会议地点：
Beijing, PEOPLES R CHINA
会议主办单位：
[Wang, Jing-song;Hu, Xin-jiang;Xie, Shui-bo;Bao, Zheng-lei] Univ S China, Sch Urban Construct, Hengyang 421001, Hunan, Peoples R China.^[Wang, Jing-song;Xie, Shui-bo] Univ S China, Hunan Key Lab Pollut Control & Resource Reuse, Henyang 421001, Hunan, Peoples R China.^[Hu, Xin-jiang;Liu, Yun-guo] Hunan Univ, Coll Environm Sci & Engn, Changsha 410082, Hunan, Peoples R China.
关键词：
Immobilized beads;Aspergillus fumigatus;Biosorption;Uranium (VI);Pseudo-second order
摘要：
Biosorption of uranium (VI) ions by immobilized Aspergillus fumigatus beads was investigated in a batch system. The influences of solution pH, biosorbent dose, U (VI) concentration, and contact time on U (VI) biosorption were studied. The results indicated that the adsorption capacity was strongly affected by the solution pH, the biosorbent dose and initial U (VI) concentration. Optimum biosorption was observed at pH 5.0, biosrobent dose (w/v) 2.5%, initial U (VI) concentration 60 mg L-1. Biosorption equilibrium was established in 120 min. The adsorption process conformed to the Freunlich and Temkin isothermal adsorption models. The dynamic adsorption model conformed to pseudo-second order model. (C) 2010 Elsevier Ltd. All rights reserved.
