激光捕获显微切割技术纯化的人支气管上皮癌变各阶段组织的定量蛋白质组学研究
作者：
Zeng Gu-Qing;Cheng Ai-Lan;Yi Hong;Li Mao-Yu;Li Guo-Qing;...
期刊：
生物化学与生物物理进展 ,2013年40(6):538-547 ISSN：1000-3282
通讯作者：
Li Jian-Ling
作者机构：
[Liao Li; Zeng Gu-Qing] Univ South China, Sch Nursing, Hengyang 421001, Peoples R China.;[Li Mao-Yu; Yi Hong; Li Jian-Ling] Cent S Univ, Xiangya Hosp, Chinese Minist Hlth, Key Lab Canc Prote, Changsha 410008, Hunan, Peoples R China.;[Li Guo-Qing; Cheng Ai-Lan] Univ South China, Canc Res Inst, Hengyang 421001, Peoples R China.
通讯机构：
[Li Jian-Ling] C;Cent S Univ, Xiangya Hosp, Chinese Minist Hlth, Key Lab Canc Prote, Changsha 410008, Hunan, Peoples R China.
关键词：
人支气管上皮癌变;肺鳞癌;生物标志物;激光捕获显微切割技术;同位素标记相对和绝对定量
摘要：
为分析支气管上皮癌变进程中的差异表达蛋白质,筛选肺鳞癌早期诊断标志物,以人支气管上皮癌变各阶段组织为研究对象,先采用激光捕获显微切割技术(LCM)纯化人正常支气管上皮组织、鳞状化生、不典型增生、原位癌、浸润性肺鳞癌组织,再用同位素标记相对和绝对定量(iTRAQ)技术结合二维液相色谱串联质谱(2D LC-MS/MS)鉴定支气管上皮癌变进程中各阶段的差异表达蛋白质.结果共鉴定了1 036个蛋白质,筛选出102个与人支气管上皮癌变相关的差异蛋白质,在这些差异蛋白质中,有的在支气管上皮癌变过程中进行性上调,有的在支气管上皮癌变过程中进行性下调,有的呈阶段特异性改变.功能分析表明,这些差异蛋白质涉及代谢、细胞凋亡、增殖、分化、信号传导、转录、翻译、细胞黏附、免疫反应与发育等.Western blotting及免疫组织化学技术验证了其中2个差异蛋白(S100A9和CKB)的表达,证实了定量蛋白质组学结果的可靠性.研究结果提示:这些差异表达蛋白质与支气管上皮癌变相关,并可成为肺鳞癌的早期诊断标志物,进一步研究差异蛋白质的生物学功能,将有助于阐明支气管上皮的癌变机制,从而为肺鳞癌的早期诊断与发病机制研究提供新思路
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中文
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基于应急能力提升的卓越公共卫生人才培养模式初探
作者：
李乐;龙鼎新;何淑雅;唐双阳;廖力;...
期刊：
中国高等医学教育 ,2012年(9):12-13+84 ISSN：1002-1701
作者机构：
南华大学公共卫生学院;南华大学医学院,湖南衡阳,421001;[让蔚清; 唐双阳; 何淑雅; 李乐; 龙鼎新; 李良; 廖力; 谢红卫] 南华大学
关键词：
应急能力;卓越;公共卫生人才
摘要：
公共卫生人才是疾病预防控制和突发公共卫生事件处置的后备骨干力量,培养应急型卓越公共卫生人才是新时期医学实践教学改革趋势,具有重要社会意义.从分析我国公共卫生类学生应急能力培养薄弱环节,解读卓越公共卫生人才内涵,加强应急教学平台建设和学科建设,构建应急型卓越公共卫生人才培养模式、优化课程体系,改革实践教学体系等入手,全面培养卓越公共卫生人才.
语种：
中文
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衡阳市公众核电认知水平分析
作者：
王一龙;廖力;何煦;王国君
期刊：
南华大学学报(社会科学版) ,2012年13(3):8-11 ISSN：1673-0755
作者机构：
南华大学公共卫生学院,湖南衡阳,421001;[何煦; 王一龙; 廖力; 王国君] 南华大学
关键词：
核电;核电认知;核电接受
摘要：
为了解衡阳市公众核电认知水平的程度，并通过分析其影响因素，为衡阳市未来核电发展提供理论依据与数据支持。采用多阶抽样法选取衡阳市5个区（红湘区、雁峰区、殊晖区、蒸湘区、石鼓区）及5个县（衡南县、衡阳县、衡山县、常宁市、衡东县），共计4200人作为研究对象。研究表明，衡阳市公众对核电相关知识掌握的总体水平偏低，其掌握知识的情况，男性高于女性；以40岁～年龄组认知水平最高；随着文化程度的增高呈增高的趋势；城市高于农村；并随个人年收入的增长而增高。
语种：
中文
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A square wave voltammetric method for the detection of microorganism populations using a MWNT-modified glassy carbon electrode
作者：
Xiao, Xilin* ;Zhu, Guizhi;Liao, Lifu;Liu, Bo;Yuan, Yali;...
期刊：
Electrochimica Acta ,2012年74:105-110 ISSN：0013-4686
通讯作者：
Xiao, Xilin
作者机构：
[Wu, Yimou; Yuan, Yali; Wang, Yongsheng; He, Jun; Xiao, Xilin; Liao, Lifu; He, Bo] Univ S China, Coll Chem & Chem Engn, Hengyang 421001, Hunan, Peoples R China.;[Wu, Yimou; Yuan, Yali; Wang, Yongsheng; He, Jun; Xiao, Xilin; Liao, Lifu; He, Bo] Univ S China, Coll Publ Hlth, Pathogen Biol Inst, Hengyang 421001, Hunan, Peoples R China.;[Zhu, Guizhi; Liu, Bo; Xiao, Xilin] Univ Florida, Ctr Res Bio Nano Interface, Dept Chem & Physiol, Gainesville, FL 32611 USA.;[Zhu, Guizhi; Liu, Bo; Xiao, Xilin] Univ Florida, Dept Funct Genom, Strands Canc Ctr, UF Genet Inst, Gainesville, FL 32611 USA.;[Zhu, Guizhi; Liu, Bo; Xiao, Xilin] Univ Florida, McKnight Brain Inst, Gainesville, FL 32611 USA.
通讯机构：
[Xiao, Xilin] U;Univ S China, Coll Chem & Chem Engn, Hengyang 421001, Hunan, Peoples R China.
关键词：
Square wave voltammetry (SWV);Cyclic voltammetry (CV);Multiwalled carbon nanotube (MWNT);Glassy carbon electrode;Escherichia coli O157:H7
摘要：
A novel method for determination of trace amounts of microorganism populations in solution was developed by using a multiwalled carbon nanotube (MWNT) modified glassy carbon electrode and square wave voltammetry (SWV). The simultaneous combination of MWNT and SWV allowed the electrochemical signal of electro-active materials in Escherichia coli O157:H7 (E. coli) to be dramatically amplified. Compared with a bare glassy carbon electrode, the MWNT-modified glassy carbon electrode showed catalytic properties in the oxidation of electro-active materials on cell surfaces. Moreover, SWV was proved to be more sensitive than cyclic voltammetry (CV) for investigation of the electrochemical behavior of cells. In this paper, a linear relationship was obtained between the SWV peak current and the cell concentration in the range 2 × 102–2 × 108 cell mL−1 with a detection limit of 2 × 102 cell mL−1. The effect of antibiotic drug Gentamycin Sulfate injection (GSI) on the growth of E. coli was also investigated.
语种：
英文
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Detection of Mycoplasma genitalium using a wireless magnetoelastic immunosensor
作者：
He, Bo;Liao, Lifu* ;Xiao, Xilin;Gao, Shuqin;Wu, Yimou
期刊：
Analytical Biochemistry ,2011年408(1):1-4 ISSN：0003-2697
通讯作者：
Liao, Lifu
作者机构：
[Gao, Shuqin; Xiao, Xilin; Liao, Lifu; He, Bo] Univ S China, Coll Chem & Chem Engn, Hengyang 421001, Hunan, Peoples R China.;[He, Bo] Univ S China, Coll Publ Hlth, Hengyang 421001, Hunan, Peoples R China.;[Xiao, Xilin] Hunan Univ, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Hunan, Peoples R China.;[Wu, Yimou] Univ S China, Inst Pathogen Biol, Hengyang 421001, Hunan, Peoples R China.
通讯机构：
[Liao, Lifu] U;Univ S China, Coll Chem & Chem Engn, Hengyang 421001, Hunan, Peoples R China.
关键词：
Immunosensor;Magnetoelastic;Mycoplasma genitalium;Wireless
摘要：
A wireless immunosensor for the detection of Mycoplasma genitalium was fabricated by immobilizing polyclonal antibody onto the surface of a magnetostrictive strip. In response to a time-varying magnetic field, the immunosensor longitudinally vibrates at a resonance frequency, emitting magnetic flux that can be remotely detected by a pickup coil. No physical connections between the immunosensor and the detection system are required, facilitating wireless aseptic operation. The binding of M. genitalium to the immunosensor surface resulted in a decrease in the resonance frequency of the immunosensor. When solutions with varying concentrations of the bacteria were tested, the shift of the resonance frequency was proportional to the concentration of M. genitalium. Under the optimized conditions, the linear range for the determination of M. genitalium was 2.0×103 to 2.9×104 color change units (ccu)/ml with a detection limit of 3.4×102 ccu/ml. The immunosensor was successfully applied to real samples containing M. genitalium with results similar to those previously obtained by the color change unit method. © 2010 Elsevier Inc.
语种：
英文
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A catalytic resonance fluorometry method for determination of hydroquinone and its applications
作者：
Li, Guirong* ;Tan, Guanghui;Liu, Yunfu;Wang, Yongsheng;Liao, Lifu
期刊：
Analytical Sciences ,2009年25(4):493-497 ISSN：0910-6340
通讯作者：
Li, Guirong
作者机构：
[Wang, Yongsheng; Tan, Guanghui; Liu, Yunfu; Li, Guirong] Univ S China, Coll Publ Hlth, Hengyang 421001, Peoples R China.;[Liao, Lifu] Univ S China, Coll Chem & Chem Engn, Hengyang 421001, Peoples R China.
通讯机构：
[Li, Guirong] U;Univ S China, Coll Publ Hlth, Hengyang 421001, Peoples R China.
摘要：
Pyronin Y (PY) has a strong resonance fluorescence in sulfuric acid medium. The characteristics of the resonance fluorescence spectra and the factors affecting the spectra were studied. A catalytic resonance fluorometry method for the determination of hydroquinone was proposed based on the catalytic effect of hydroquinone on the oxidation of PY by potassium bromate. The oxidation of PY resulted in the decrease of the resonance fluorescence intensity. The influences of several variables on the sensitivity were studied. At the optimized conditions, the decrease of the resonance fluorescence intensity was in proportion to the concentration of hydroquinone in the range of 4.42 - 1.60 × 103 μg l-1, and the detection limit was 1.46 μg l-1. The proposed method was applied successfully for the determination of trace hydroquinone in environment samples. The relative standard deviation was less than 3.90% and the recoveries were in the range of 95.2 - 104.0%. 2009 © The Japan Society for Analytical Chemistry.
语种：
英文
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Monitoring of Mycoplasma genitalium growth and evaluation of antibacterial activity of antibiotics tetracycline and levofloxacin using a wireless magnetoelastic sensor
作者：
He, Bo;Liao, Lifu* ;Xiao, Xilin;Gao, Shuqin;Wu, Yimou
期刊：
Biosensors and Bioelectronics ,2009年24(7):1990-1994 ISSN：0956-5663
通讯作者：
Liao, Lifu
作者机构：
[Gao, Shuqin; Xiao, Xilin; Liao, Lifu; He, Bo] Univ S China, Coll Chem & Chem Engn, Hengyang 421001, Hunan, Peoples R China.;[Liao, Lifu; He, Bo] Univ S China, Coll Publ Hlth, Hengyang 421001, Hunan, Peoples R China.;[Xiao, Xilin] Hunan Univ, State Key Lab Chemobiosensing & Chemometr, Changsha 410082, Hunan, Peoples R China.;[Wu, Yimou] Univ S China, Inst Pathogen Biol, Hengyang 421001, Hunan, Peoples R China.;[Liao, Lifu] Univ S China, Coll Chem & Chem Engn, Xueyan Rd, Hengyang 421001, Hunan, Peoples R China.
通讯机构：
[Liao, Lifu] U;Univ S China, Coll Chem & Chem Engn, Xueyan Rd, Hengyang 421001, Hunan, Peoples R China.
关键词：
Magnetoelastic sensor;Mycoplasma genitalium;Wireless;Tetracycline;Levofloxacin
摘要：
Mycoplasma genitalium (Mg) is the smallest and simplest self-replicating bacteria lacking of cell wall and is a human pathogen causing various diseases. This paper describes the real-time, long-term and in situ monitoring of the growth of Mg and evaluation of the effect of the antibiotics tetracycline and levofloxacin on the growth using a wireless magnetoelastic sensor. The sensor is fabricated by coating a magnetoelastic strip with a polyurethane protecting film. In response to a time-varying magnetic field, the sensor longitudinally vibrates at a resonance frequency, emitting magnetic flux that can be remotely detected by a pick-up coil. No physical connections between the sensor and the detection system are required. The wireless property facilitates aseptic operation. The adhesion of Mg on the sensor surface results in a decrease in the resonance frequency, which is proportional to the concentration of Mg. The shift of the resonance frequency-time curves shows that under routine culture condition the growth curve of Mg is composed of three phases those are lag, logarithmic and stationary phase, respectively. In the presence of the antibiotics, the lag phase in the growth inhibition curves is prolonged obviously and the stationary phase is substituted by a decline phase. The growth inhibition of Mg is related to the concentration of the antibiotics. The MIC50 (minimal inhibitory concentration) of Mg incubated in the presence of the antibiotics for 120 h is calculated to be 1.5 and 0.5 μg/mL for tetracycline and levofloxacin, respectively. © 2008 Elsevier B.V. All rights reserved.
语种：
英文
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The molecular resonance fluorescence method for determination of arsenic in hair samples
作者：
Li, Guirong* ;Liu, Yunfu;Wang, Yongsheng;Liao, Lifu
期刊：
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY ,2009年72(4):811-815 ISSN：1386-1425
通讯作者：
Li, Guirong
作者机构：
[Wang, Yongsheng; Liu, Yunfu; Li, Guirong] Univ S China, Coll Publ Hlth, Hengyang 421001, Peoples R China.;[Liao, Lifu] Univ S China, Coll Chem & Chem Engn, Hengyang 421001, Peoples R China.
通讯机构：
[Li, Guirong] U;Univ S China, Coll Publ Hlth, Hengyang 421001, Peoples R China.
关键词：
Molecular resonance fluorescence;Arsenic;Rhodamine B;Hair samples
摘要：
A molecular resonance fluorescence quenching method for the determination of arsenic was proposed. The method is based on the quenching effect of As (V) on the molecular resonance fluorescence of Rhodamine B (RhB) in sulfuric acid medium and in the presence of molybdate and PVA. The influences of acidity, chemical and manifold variables on the sensitivity were studied. At the optimized conditions, the linear range for the determination of arsenic was 2.0 ×10<sup>-3</sup>-0.12 μg ml<sup>-1</sup> with the detection limit of 1.20 ×10<sup>-9</sup> g ml<sup>-1</sup>. The relative standard deviations for the eleven replicate determinations of 0.006 μg ml<sup>-1</sup> and 0.060 μg ml<sup>-1</sup> of arsenic were 1.12% and 1.61%, respectively. The proposed method was applied successfully for the determination of trace arsenic in hair samples with the recoveries of 94.8-104.6%. ©2008 Elsevier B.V. All rights reserved.
语种：
英文
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Determination of Sudan I in chilli powder from solvent components gradual change–visible spectra data using second order calibration algorithms
作者：
Yuan, Jintao;Liao, Lifu* ;Lin, Yingwu;Deng, Changai;He, Bo
期刊：
Analytica Chimica Acta ,2008年607(2):160-167 ISSN：0003-2670
通讯作者：
Liao, Lifu
作者机构：
[Yuan, Jintao; Liao, Lifu; Lin, Yingwu; Deng, Changai] Univ S China, Coll Chem & Chem Engn, Hengyang 421001, Hunan, Peoples R China.;[Yuan, Jintao; He, Bo] Univ S China, Coll Publ Hlth, Hengyang 421001, Hunan, Peoples R China.
通讯机构：
[Liao, Lifu] U;Univ S China, Coll Chem & Chem Engn, Hengyang 421001, Hunan, Peoples R China.
关键词：
Parallel factor analysis;Rank annihilation factor analysis;Second order calibration;Solvent components gradual change;Sudan I;Visible absorption spectra
摘要：
It was discovered that a second order spectra data matrix of Sudan I produced from the solvent components gradual change-visible absorption spectra can be expressed as the combination of two bilinear data matrices. Based on this discovery, a new method for the determination of Sudan I in gray systems using second order calibration algorithms has been developed. The second order calibration algorithms were based on the popular parallel factor analysis (PARAFAC) and rank annihilation factor analysis (RAFA), respectively. In the method described here, the components of the solvent were changed gradually by adding ethanol into cyclohexane, the absorption spectra of Sudan I and chilli samples in a series of cyclohexane-ethanol mixed solvents with various ethanol volume fractions were recorded, and then the second order data were obtained from the solvent components gradual change-visible absorption spectra. Thus, the concentration of Sudan I in a gray system could be determined from the spectra matrices using second order calibration algorithms. This method is simple, convenient and dependable. The method has been used to determine Sudan I in chilli powder with satisfactory results. © 2007 Elsevier B.V. All rights reserved.
语种：
英文
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贫困大学生心理健康状况的调查与分析
作者：
陈雯;陈新;王国平;廖力
期刊：
中国健康心理学杂志 ,2008年16(6):631-634 ISSN：2096-4811
作者机构：
中国,南华大学公共卫生学院,湖南衡阳,421001;[陈新; 廖力; 王国平; 陈雯] 南华大学
关键词：
心理健康;贫困大学生;调查;症状自评量表
摘要：
目的 了解贫困大学生的心理健康状况.方法 采用症状自评量表(SCL-90)和大学生人格健康调查表(UPI)对2882名贫困生和8337名非贫困生进行问卷调查与对比研究.结果 贫困生的9个因子分高于非贫困生;1～3年级中,二年级贫困生的各项因子分最高;某些因子分,农村贫困生高于城市贫困生,单亲家庭贫困生高于双亲家庭贫困生,不和谐家庭中的贫困生高于和谐家庭中的贫困生.结论 贫困生整体心理健康水平,低于非贫困生;除经济条件外,贫困生的家庭来源地、结构、氛围等背景条件也是影响心理健康的重要因素.
语种：
中文
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南华大学学生心理健康状况调查分析
作者：
陈雯;廖力;王国平;陈新
期刊：
中国全科医学 ,2008年11(4):348-350+354 ISSN：1007-9572
作者机构：
湖南省南华大学公共卫生学院,湖南省衡阳市,421001
关键词：
心理健康;大学生
摘要：
目的 了解南华大学学生心理健康状况,为学校开展心理健康教育和进行有针对性的心理干预提供依据.方法 在校园网上,采用"大学生心理测验软件(5.0网络版)",对南华大学一至三年级的11 219名本科生进行临床症状自评量表(SCL-90)和大学生人格健康调查表(UPI)问卷调查.结果 南华大学学生SCL-90中的躯体化、强迫症状、人际关系、抑郁、焦虑、敌对、恐怖、偏执、精神病性因子分与国内青年常模比较,差别有统计学意义(P＜0.01).男生和女生的强迫症状、人际关系、抑郁、敌对、恐怖、偏执、精神病性因子分间差别有统计学意义(P＜0.05).不同年级学生SCL-90各因子分间差别均有统计学意义(P＜0.01);除二年级学生在躯体化、敌对、偏执和其他因子方面得分分与三年级的比较无统计学意义(P＞0.05)外,其余各因子分3个年级间两两比较差别均有统计学意义(P＜0.05).独生子女除敌对因子分外,其余各因子分与非独生子女比较,差别均有统计学意义(P＜0.05).检测出有明显心理问题,须尽快进行心理咨询的人数为2544(占22.7%).结论 南华大学在校大学生整体心理健康水平虽然高于国内青年常模,但不同性别和年级间差别显著;而且还存在一些不容忽视的心理健康问题.
语种：
中文
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