仪器分析教学中任务驱动式教学法的应用
作者：
徐小娜;蒋军辉;杨慧仙;于军晖;李贵荣
期刊：
山西医科大学学报 ,2012年14(3):189-191 ISSN：1007-6611
作者机构：
南华大学公共卫生学院卫生检验系,衡阳,421001;南华大学化学化工学院高分子材料系;[徐小娜; 杨慧仙; 李贵荣; 蒋军辉; 于军晖] 南华大学
关键词：
仪器分析;教学方法;任务驱动
摘要：
分析仪器分析课程教学中存在的问题,尝试在仪器分析教学中应用任务驱动式教学法,以期全面提高教学质量,为后续课程的学习和培养高素质的应用型人才奠定基础。
语种：
中文
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红树林内源真菌Fusarium sp.#ZZF51吸附Cu~(~(2+))的研究
作者：
阳海斌;谭倪;邓昌爱;孙曼;佘志刚;...
期刊：
中山大学学报(自然科学版)(中英文) ,2012年51(1):63-66 ISSN：0529-6579
通讯作者：
Tan, N.
作者机构：
[阳海斌; 谭倪; 邓昌爱; 孙曼] 南华大学化学化工学院;[佘志刚; 林永成] 中山大学化学与化学工程学院
通讯机构：
School of Chemistry and Chemical Engineering, University of South China, China
关键词：
红树林内源真菌;生物吸附
摘要：
采用生物吸附法去除废水中Cu~(2+),研究了南海红树林内源真菌Fusarium sp.#ZZF51吸附Cu~(2+)的行为特性、吸附模型及吸附机理。结果表明:受试菌吸附Cu~(2+)的吸附平衡时间为90 min,常温常压下吸附最佳pH值为6.5,Cu~(2+)初始浓度50 mg/L,吸附时间90 min,此时受试菌对Cu~(2+)的吸附率和吸附容量分别为82.14%和20.53 mg/g。Langmuir方程比Freundlich方程能更好地描述该受试菌对Cu~(2+)的平衡吸附行为。通过比较受试菌吸附Cu~(2+)前后红外光谱图得知,在吸附过程中,羟基和羰基都起着十分重要的作用。
语种：
中文
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普通高校双语教学的实践与思考──南华大学有机化学双语教学的个案研究
作者：
邓健;刘慧玲;魏传晚
期刊：
广东化工 ,2012年39(4):217-218 ISSN：1007-1865
作者机构：
南华大学化学化工学院,湖南衡阳,421001
关键词：
双语教学;有机化学;高等教育
摘要：
通过个案研究南华大学有机化学双语教学实践,从双语教学模式、师资要求、教材选用和学生素质几个方面,探讨了高校双语教学面临的问题及对策。
语种：
中文
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Electrochemical sensor based on a poly(para-aminobenzoic acid) film modified glassy carbon electrode for the determination of melamine in milk
作者：
Liu, Ya Ting;Deng, Jian* ;Xiao, Xi Lin;Ding, Li;Yuan, Ya Li;...
期刊：
Electrochimica Acta ,2011年56(12):4595-4602 ISSN：0013-4686
通讯作者：
Deng, Jian
作者机构：
[Wang, Li Li; Li, Xiu Ting; Liu, Ya Ting; Yan, Xiao Na; Li, Hui; Deng, Jian; Yuan, Ya Li; Xiao, Xi Lin] Univ S China, Sch Chem & Chem Engn, Hengyang 421001, Hunan, Peoples R China.;[Ding, Li] Hunan Prov Ctr Dis Prevent & Control, Changsha 410005, Hunan, Peoples R China.;[Deng, Jian] Univ S China, Sch Chem & Chem Engn, 28 Changsheng W Rd, Hengyang 421001, Hunan, Peoples R China.
通讯机构：
[Deng, Jian] U;Univ S China, Sch Chem & Chem Engn, 28 Changsheng W Rd, Hengyang 421001, Hunan, Peoples R China.
关键词：
Molecularly imprinted polymer;Poly(para-aminobenzoic acid);Electropolymerization;Melamine;Milk
摘要：
A novel and simple sensor is developed in this paper for melamine detection, which is based on an electropolymerized molecularly imprinted polymer (MIP) of para-aminobenzoic acid (pABA). The poly(para-aminobenzoic acid) (P-pABA) film was deposited in a pABA solution by potentiodynamic cycling of potential with and without the template (melamine) on a glassy carbon electrode. The surface feature of the modified electrode was characterized by electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV). The molecular imprinted sensor was tested by differential pulse voltammetry (DPV) to verify the changes in redox peak currents of hexacyanoferrate. Several important parameters controlling the performance of the P-pABA were investigated and optimized. In the optimal conditions, the relative redox peak currents of hexacyanoferrate were linear. The concentration of melamine ranged from 4.0 μM to 0.45 mM, with a linear correlation coefficient of 0.9992. The detection limit was 0.36 μM (S/N = 3). The MIP sensor was successfully applied to the determination of melamine in milk products and showed high selectivity, sensitivity, and reproducibility. The results of this research demonstrate that it is feasible to use the molecular imprinting methodology when preparing sensing devices for analytes that are electrochemically inactive. © 2011 Elsevier Ltd. All rights reserved.
语种：
英文
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A Dehydrogenative Homocoupling Reaction for the Direct Synthesis of Hydrazines from N-Alkylanilines in Air
作者：
Yan, Xue-Ming;Chen, Zhi-Ming;Yang, Fei;Huang, Zhi-Zhen*
期刊：
SYNLETT ,2011年2011(4):569-572 ISSN：0936-5214
通讯作者：
Huang, Zhi-Zhen
作者机构：
[Yan, Xue-Ming; Chen, Zhi-Ming; Huang, Zhi-Zhen; Yang, Fei] Nanjing Univ, Coll Chem & Chem Engn, Nanjing 210093, Peoples R China.;[Huang, Zhi-Zhen] Nankai Univ, Key Lab Elementoorgan Chem, Tianjin 300071, Peoples R China.;[Yan, Xue-Ming] Univ S China, Coll Chem & Chem Engn, Hengyang 421001, Hunan, Peoples R China.
通讯机构：
[Huang, Zhi-Zhen] N;Nanjing Univ, Coll Chem & Chem Engn, Nanjing 210093, Peoples R China.
关键词：
homocoupling;oxidative dehydrogenation;copper catalyst;direct synthesis;hydrazine
摘要：
A copper-catalyzed N-N bond-forming reaction was performed by a dehydrogenative homocoupling of N-alkylanilines, affording N,N-dialkyl-N,N- diphenylhydrazines in 72-88% yields. This new strategy has the advantages of direct synthesis from N-alkylanilines, using air as the oxidant, convenient manipulations, mild reaction conditions and moderate to good yields. A possible mechanism by coordination and reductive elimination has been proposed. © Georg Thieme Verlag Stuttgart New York.
语种：
英文
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Comparative proteomic analysis of cellular response of human airway epithelial cells (A549) to benzo(a)pyrene
作者：
Min, Lingfeng;He, Shuya;Chen, Qiong* ;Peng, Fang;Peng, Hongbing;...
期刊：
Toxicology Mechanisms and Methods ,2011年21(5):374-382 ISSN：1537-6516
通讯作者：
Chen, Qiong
作者机构：
[Xie, Mingxuan; Min, Lingfeng; Chen, Qiong; Peng, Hongbing] Cent S Univ, Dept Geriatr Med, Dept Resp, Xiangya Hosp, Changsha 410008, Hunan, Peoples R China.;[He, Shuya] Univ S China, Dept Biochem & Mol Biol, Hengyang, Peoples R China.;[Peng, Fang] Cent S Univ, Key Lab Canc Prote, Minist Hlth China, Xiangya Hosp, Changsha 410008, Hunan, Peoples R China.
通讯机构：
[Chen, Qiong] C;Cent S Univ, Dept Geriatr Med, Dept Resp, Xiangya Hosp, Changsha 410008, Hunan, Peoples R China.
关键词：
A549;B(a)P;Mn SOD;mass spectrometry;mitochondrial dysfunction;two-dimensional gel electrophoresis
摘要：
This work aimed to investigate the cellular response of human airway epithelial cells (A549) to oxidative stress induced by benzo(a)pyrene [B(a)P]. Levels of intracellular reactive oxygen species (ROS) and lipid peroxidation were investigated in A549 cells treated with varying concentrations of B(a)P. A comparative proteomic analysis of total proteins was performed in cells treated with 1 μM B(a)P [B(a)P-1] and untreated cells. The expression of Mn superoxide dismutase (Mn SOD), one of the identified down-regulated proteins in B(a)P-1 cells, was then analyzed by Western blotting. The total antioxidant activity, total superoxide dismutase activity, catalase (CAT) activity, and glutathione reductase (GR) activity were all analyzed after B(a)P treatment. Our results demonstrated that 1 μM B(a)P could induce ROS generation and lead to lipid peroxidation in A549 cells, and 23 differentially expressed proteins were identified. The expression levels of Mn SOD and the total SOD were induced at 0.1 μM and suppressed at 1 μM and 10 μM. Up-regulation of CAT and GR activity resulted in an increase in total antioxidant activity in A549 after exposure to B(a)P. These findings provide a basis for understanding the mechanisms of mitochondrial dysfunction and perturbation of antioxidant status induced by B(a)P on airway epithelial cells. © 2011 Informa Healthcare USA, Inc.
语种：
英文
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不同方法制备的Cu/HZSM-5催化剂上NO的催化分解反应
作者：
阳鹏飞;周继承;任文明
期刊：
燃料化学学报 ,2011年39(2):122-127 ISSN：0253-2409
通讯作者：
Yang, P.-F.(ypengfei2008@126.com)
作者机构：
[周继承; 任文明; 阳鹏飞] Key Laboratory of Green Catalysis and Chemical Reaction Engineering in College of Hunan Province, School of Chemistry and Chemical Engineering, Xiangtan University, Xiangtan 411105, China;[阳鹏飞] School of Chemistry and Chemical Engineering, The South China of University, Hengyang 421001, China
关键词：
Cu/HZSM-5分子筛;一氧化氮;催化分解;制备方法
摘要：
采用离子交换法、固相分散法和微波固相法等不同方法制备了Cu/HZSM-5催化剂,以BET、XRD和XPS等手段对催化剂样品进行了表征。结果表明,不同方法制备的Cu/HZSM-5催化剂上Cu物种的落位分布状态不同,离子交换法制备的催化剂Cu物种更多地落位于分子筛孔道内,微波固相法和固相分散法制备的催化剂Cu物种较多地落位分布在分子筛外表面。固相分散法制备的样品未能使铜物种完全分散于分子筛表面,在13.1°、16.8°、35.5°和38.0°等处仍存在CuO的晶相衍射峰。催化分解NO反应的活性考察结果表明,用微波固相法制备的催化剂催化分解NO的活性及稳定性明显超过另两种方法所制备的催化剂,在无氧条件下NO最初转化率高达89.2%,经反应25h后,转化率仍维持在70%以上;在富氧气氛下催化分解NO活性降低速率低于由离子交换法制备的催化剂。结合表征结果可以得出,落位于分子筛外表面以离子交换态形式存在的Cu物种对催化分解NO反应更为有利,而且催化稳定性更好。
语种：
中文
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探讨激发学生学习医学基础化学兴趣的方法
作者：
王晓娟;魏传晚;刘慧君;邓昌爱;杨泽权;...
期刊：
化工时刊 ,2011年25(2):68-70 ISSN：1002-154X
作者机构：
南华大学化学化工学院,湖南,衡阳,421001;[丁娟; 杨泽权; 邓昌爱; 刘慧君; 魏传晚; 王晓娟] 南华大学
关键词：
学习态度;激发;学习兴趣;方案
摘要：
通过对几年来医学类大学生学习基础化学的现状研究,分析了学生学生中间环节学习态度比较散漫的成因;结合医用基础化学课程的特点,提出了激发学生学习基础化学兴趣的几种方案。
语种：
中文
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液相链烷烃热导率与其结构定量关系
作者：
彭国文;肖方竹;聂长明;廖力夫;杨胜园;...
期刊：
化工学报 ,2011年62(3):604-610 ISSN：0438-1157
通讯作者：
Peng, G.(pgwnh@sohu.com)
作者机构：
[廖力夫; 聂长明; 肖锡林; 彭国文] School of Chemistry and Chemical Engineering, University South China, Hengyang 421001, Hunan, China;[杨胜园; 肖方竹] School of Public Health, University South China, Hengyang 421001, Hunan, China;[聂长明] Laboratory of Quantum Chemistry and Physics, University of Paul Sabatier(Toulouse-III), Toulouse 31077, France;[杨胜园] State Key Laboratory of Chemo-Biosensing and Chemometrics, Hunan University, Changsha 410082, Hunan, China
通讯机构：
School of Chemistry and Chemical Engineering, University South China, China
关键词：
液相链烷烃;热导率;平衡电负性;拓扑模型;定量关系
摘要：
应用密度泛函理论(DFT),在B3LYP/6-31+G(d)水平上对含有5～24个碳原子的部分液相链烷烃进行结构优化和量化计算其分子中各个原子间空间拓扑距离,并构建拓扑距离矩阵.结合分子中各原子的支化度,应用原子的平衡电负性对分子图进行着色,得到新型结构拓扑指数PX<,m>(m=1,2,3).采用多元线性回归技术建立液相链烷烃热导率γ与PX[m](m=1,2,3)的定量关系拓扑模型,并用该模型对热导率γ进行预测与估算,结果表明实验值与预测值、估算值均很吻合.同时,采用留一法(1eave-one-out)和外检验方法测试模型的内部稳定性和外部预测能力.与文献结果比较,本文所用参数少,计算简便,为液相链烷烃导热的研究提供了一种新方法.
语种：
中文
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化学修饰啤酒酵母菌对铀的吸附特性
作者：
彭国文;丁德馨;胡南;杨雨山;王晓亮
期刊：
化工学报 ,2011年62(11):3201-3206 ISSN：0438-1157
通讯作者：
Ding, D.(dingdxzzz@163.com)
作者机构：
School of Resources and Safety Engineering, Central South University, Changsha 410083, Hunan, China;[丁德馨; 胡南] Key Discipline Lab. for National Defence for Biotechnology in Uranium Mining and Hydrometallurgy, University of South China, Hengyang 421001, Hunan, China;[王晓亮] School of Chemistry and Chemical Engineering, University of South China, Hengyang 421001, Hunan, China;[彭国文] School of Resources and Safety Engineering, Central South University, Changsha 410083, Hunan, China<&wdkj&>Key Discipline Lab. for National Defence for Biotechnology in Uranium Mining and Hydrometallurgy, University of South China, Hengyang 421001, Hunan, China<&wdkj&>School of Chemistry and Chemical Engineering, University of South China, Hengyang 421001, Hunan, China;[杨雨山] School of Resources and Safety Engineering, Central South University, Changsha 410083, Hunan, China<&wdkj&>Key Discipline Lab. for National Defence for Biotechnology in Uranium Mining and Hydrometallurgy, University of South China, Hengyang 421001, Hunan, China
通讯机构：
Key Discipline Lab. for National Defence for Biotechnology in Uranium Mining and Hydrometallurgy, University of South China, China
关键词：
修饰啤酒酵母;化学修饰;铀;生物吸附;动力学模型
摘要：
以甲醛为交联剂,将胱氨酸修饰到啤酒酵母菌(SC)上,并采用海藻酸钠和明胶固定化,得到一种新型的生物吸附剂——修饰啤酒酵母菌( MSC).通过红外光谱(IR)分别表征了两种吸附剂的结构,考察了其吸附铀的主要影响因素即溶液pH值、吸附时间等.结果表明:MSC细胞表面具有大量吸附铀的基团,MSC和SC吸附铀的最佳条件是:pH值为6.0,吸附时间分别为1.8、1.5h.对吸附动力学模型和吸附等温模型进行了分析,MSC和SC对铀的吸附动力学模型较好地符合了准二级动力学模型,MSC和SC的相关系数均大于0.99;吸附等温线均能符合Langmuir和Freundlich等温线模型,说明该吸附体系是一个单层覆盖与多层吸附相结合的吸附模式,且MSC的最大吸附量是SC的6.5倍.
语种：
中文
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量子拓扑指数法预测多氯联苯醚的热力学性质
作者：
肖方竹;彭国文;聂长明;杨胜园
期刊：
化工学报 ,2011年62(7):1808-1816 ISSN：0438-1157
通讯作者：
Peng, G.(pgwnh@sohu.com)
作者机构：
[聂长明; 肖方竹] School of Public Health, University South China, Hengyang 421001, Hunan, China;[杨胜园; 彭国文] School of Chemistry and Chemical Engineering, University South China, Hengyang 421001, Hunan, China;[聂长明] Laboratory of Quantum Chemistry and Physics, University of Paul Sabatier (Toulouse-III), Toulouse 31077, France;[杨胜园] State Key Laboratory of Chemo-Biosensing and Chemometrics, Hunan University, Changsha 410082, Hunan, China
通讯机构：
School of Chemistry and Chemical Engineering, University South China, China
关键词：
量子拓扑指数;多氯联苯醚;热力学性质;平衡电负性;拓扑模型;定量结构-性质相关
摘要：
应用密度泛函理论(DFT),在B3LYP/6-31+G(d)基组上优化和振动分析计算了多氯联苯醚(PCDEs)所有209种可能的分子空间几何结构,得到其各原子之间空间拓扑距离,并建立拓扑空间距离矩阵.结合分子中各原子的支化度,应用原子的平衡电负性对分子图进行着色,得到量子拓扑指数PX<,1>、PX<,2>.采用多元线性回归技术建证联苯醚和209种可能结构的多氯联苯醚PCDEs 3种少见报道的热力学性质--标准生成热、标准生成自由能和相对自由能与PX<,1>、PX<,2>的定量关系拓扑模型,并用该模型分别对不同热力学性质进行预测与估算.结果表明,本方法的预测值、估算值与文献[3]的计算值均相吻合.同时,采用留一法(leave-one-out)和外检验方法测试模型的内部稳定性和外部预测能力,测试结果显示模型具有良好的稳定性和较强的预测能力.
语种：
中文
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铅(Ⅱ)离子印迹聚合物的制备及其吸附性能的研究
作者：
李路娟;刘慧君;张磊
期刊：
化学通报 ,2011年74(6):539-544 ISSN：0441-3776
通讯作者：
Liu, H.
作者机构：
[李路娟; 刘慧君; 张磊] College of Chemistry and Chemical Engineering, University of South China, Hengyang, Hunan 421001, China
通讯机构：
[Liu, H.] C;College of Chemistry and Chemical Engineering, University of South China, China
关键词：
铅离子;印迹聚合物;吸附
摘要：
利用分子印迹技术制备了重金属铅(Ⅱ)的印迹聚合物,并对其识别性能进行定量描述。以铅(Ⅱ)离子为模板,水杨醛肟为功能单体,二甲基丙烯酸乙二醇酯为交联剂,偶氮二异丁腈为引发剂,采用本体聚合法制备印迹聚合物。结果表明,与未印迹的聚合物相比较,印迹聚合物对铅(Ⅱ)离子具有较好的识别选择性,达到吸附平衡的时间为40min,Pb2+初始浓度为10mg/L时,最大吸附量为949μg/g,并推测了该印迹聚合物和铅离子的识别机理。
语种：
中文
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免疫亲和柱——高效液相色谱法检测乳制品中玉米赤霉醇及其类似物
作者：
张清杰;谢洁;果旗;江帆;王彦斐;...
期刊：
中国乳品工业 ,2011年39(12):34-35,46 ISSN：1001-2230
作者机构：
南华大学化学化工学院．湖南衡阳421001;[王彦斐; 果旗; 江帆; 王雄] 北京中检维康技术有限公司;[聂长明; 谢洁; 张清杰] 南华大学
关键词：
玉米赤霉醇;类似物;高效液相色谱法;免疫亲和柱
摘要：
采用高效液相色谱法检测牛奶和奶粉中的6种玉米赤霉醇及其类似物，该方法准确、可靠、灵敏度高。样品用乙腈提取，经免疫亲和柱净化后．用高效液相色谱-紫外检测器检测。结果表明，牛奶和奶粉中添加6种玉米赤霉醇及其类似物回收率均为80％-110％，变异系数均小于12％。
语种：
中文
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二苯乙烯苷对氧化诱导内皮细胞凋亡及Caspase-3和PARP表达的影响
作者：
Long Shi-Yin;Zhang Cai-Ping;Qiao Xin-Hui;Huang Liang-Zhu;Tian Ying* ;...
期刊：
生物化学与生物物理进展 ,2011年38(11):1052-1059 ISSN：1000-3282
通讯作者：
Tian Ying
作者机构：
[Zhang Cai-Ping; Tian Ying; Gao Xi-Qiang; Huang Liang-Zhu; Tong Li; Long Shi-Yin; Qiao Xin-Hui] Univ S China, Dept Biochem & Mol Biol, Hengyang 421001, Peoples R China.
通讯机构：
[Tian Ying] U;Univ S China, Dept Biochem & Mol Biol, Hengyang 421001, Peoples R China.
关键词：
二苯乙烯苷;细胞凋亡;多聚腺苷酸二磷酸核糖基聚合酶(PARP)
摘要：
为研究二苯乙烯苷对过氧化氢(H_2O_2)诱导人脐静脉内皮细胞凋亡的影响并初步探讨二苯乙烯苷抗凋亡的可能机制,采用不同浓度H_2O_2和二苯乙烯苷处理内皮细胞24 h,以MTT法检测细胞生长活力、Hoechst33258染色观察细胞形态、流式细胞仪检测凋亡率等方法筛选造模内皮细胞凋亡的H_2O_2浓度和二苯乙烯苷最佳的抗内皮细胞凋亡作用浓度.RT-PCR、Western-blot分别检测Caspase-3、PARP mRNA及蛋白质的表达.结果发现,与空白对照组相比,300μmol/L H_2O_2作用后,内皮细胞增殖明显受到抑制,Hoechst33258染色可见大量凋亡细胞,细胞凋亡率显著增加,流式细胞仪检测出明显的凋亡峰,Caspase-3和多聚腺苷酸二磷酸核糖基聚合酶(PARP)的表达量显著增加.经二苯乙烯苷处理后,随着二苯乙烯苷浓度增加,细胞的增殖率随之增加,凋亡细胞数减少,凋亡率逐渐降低,与H_2O_2组比较,10μmol/L的二苯乙烯苷能够显著提高细胞增殖率,降低细胞凋亡率,并显著减少Caspase-3和PARP表达.以上结果表明,二苯乙烯苷能够抑制由H_2O_2诱导的人脐静脉内皮细胞凋亡,增加细胞生长活性,降低细胞凋亡率,其作用机制可能与下调Caspase-3和多聚腺苷酸二磷酸核糖基聚合酶(PARP)的表达有关。
语种：
中文
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Rh(III)-Catalyzed Oxidative Olefination of N-(1-Naphthyl)sulfonamides Using Activated and Unactivated Alkenes
作者：
Li, Xuting;Gong, Xue;Zhao, Miao;Song, Guoyong;Deng, Jian;...
期刊：
Organic Letters ,2011年13(21):5808-5811 ISSN：1523-7060
通讯作者：
Li, Xingwei
作者机构：
[Song, Guoyong; Li, Xuting; Gong, Xue; Zhao, Miao; Li, Xingwei] Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China.;[Li, Xuting; Deng, Jian] Univ S China, Sch Chem & Chem Engn, Hengyang 421001, Peoples R China.
通讯机构：
[Li, Xingwei] C;Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China.
摘要：
Rhodium(III)-catalyzed oxidative olefination of N-(1-naphthyl)sulfonamides has been achieved at the peri position. Three categories of olefins have been successfully applied. Activated olefins reacted to afford five-membered azacycles as a result of oxidative olefination-hydroamination. Unactivated olefins reacted to give the olefination product. 2-fold oxidative C-C and C-N coupling was achieved for allylbenzenes. © 2011 American Chemical Society.
语种：
英文
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A Generic Single-tube Extraction/Partition-Multimatrix Adsorption Trapping (SEPMAT) Multiresidue Procedure for Direct Injection Liquid Chromatography-Mass Spectrometry
作者：
Chunlin Huang;Bin Guo;Xiaoying Wang;Weitao Zhu;Bo Chen;...
作者机构：
[Chunlin Huang] Department of Biochemistry and Molecular Biology, University of South China, 28 Changsheng West Road, Hengyang 421001, China;[Bin Guo; Xiaoying Wang; Weitao Zhu; Bo Chen; Shouzhuo Yao] Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education of China), College of Chemistry and Chemical Engineering, Hunan Normal University, 36 Lushan Road, Changsha 410081, China
会议名称：
第2届大连国际色谱学术报告会、第37届国际高效液相色谱及相关技术会议、第18届全国色谱学术报告会
会议时间：
2011-10-8
会议地点：
大连
会议主办单位：
中国化学会
会议论文集名称：
第2届大连国际色谱学术报告会、第37届国际高效液相色谱及相关技术会议、第18届全国色谱学术报告会论文集
关键词：
Extraction;Liquid chromatography-mass spectrometry;Multiresidue
摘要：
Notwithstanding the continuous progress in instrument's playability and impressive potential of modem LC-MS based bio-analytical methodologies, sample preparation still remains as the key technical bottleneck step, mainly due to the time-consuming and error-prone process that is highly dependent on the analyte nature and the matrix type.Nowadays, there is a clear trend in the field of residue analysis toward the use of a straightforward generic method to cover a broad array of analyte/matrix combinations by the simultaneous extraction of a vast number of target compounds (multi-analyte) from various types of sample matrixes (multi-matrix) in a single procedure.
语种：
英文
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乙二胺和聚乙二醇在纳米介孔氧化铝制备中的协同导向作用
作者：
Sun Yunkai* ;Xiao Xinrong;Yang Pengfei;Sun Zhongxi
期刊：
化学学报 ,2011年69(23):2807-2811 ISSN：0567-7351
通讯作者：
Sun Yunkai
作者机构：
[Sun Yunkai; Xiao Xinrong; Yang Pengfei] Univ S China, Sch Chem & Chem Engn, Hengyang 421001, Peoples R China.;[Sun Yunkai; Sun Zhongxi] Univ Jinan, Sch Chem & Chem Engn, Jinan 250022, Peoples R China.
通讯机构：
[Sun Yunkai] U;Univ S China, Sch Chem & Chem Engn, Hengyang 421001, Peoples R China.
关键词：
乙二胺;介孔氧化铝;协同模板
摘要：
以无机铝盐A1（NO_3）_3为原料，以乙二胺和聚乙二醇为协同模板导向剂，与氨水反应，经过“沉淀-超声-煅烧”等过程，制得介孔纳米氧化铝。用TEM， XRD，N_2吸附-脱附等测试技术对样品进行了测试和表征。研究结果表明，所得样品具有相当高的比表面积（510 m~2?G~(-1)）、较大孔容（1.13 cm~3?g~(-1)）和较为集中的孔径（5.8 nm）分布。根据乙二胺和氧化铝表面的溶液化学反应及在不同pH条件下的溶液组分分布，探讨了它们在介孔氧化铝制备过程中的协同模板作用机理。
语种：
中文
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Structural insights into a low-spin myoglobin variant with bis-histidine coordination from molecular modeling
作者：
Lin, Ying-Wu*
期刊：
PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS ,2011年79(3):679-684 ISSN：0887-3585
通讯作者：
Lin, Ying-Wu
作者机构：
[Lin, Ying-Wu] Univ S China, Sch Chem & Chem Engn, Hengyang 421001, Peoples R China.
通讯机构：
[Lin, Ying-Wu] U;Univ S China, Sch Chem & Chem Engn, Hengyang 421001, Peoples R China.
关键词：
six-coordinate;low-spin heme;hydrogen bond;protein design;MD simulation
摘要：
Rational design of functional enzymes is a powerful strategy to gain deep insights into more complex native enzymes, such as nitric oxide reductase (NOR). Recently, we engineered a functional model of NOR by creating a two His and one Glu (2-His-1-Glu) non-heme iron center in sperm whale myoglobin (swMb L29E, F43H, H64, called FeBMb(-His)). It was found that FeBMb(-His) adopts a low-spin state with bis-His coordination in the absence of metal ions binding to the designed metal center. However, no structural information was available for the variant in this special spin state. We herein performed molecular modeling of FeBMb(-His) and compared with the X-ray structure of its copper bound derivative, Cu(II)-CN−-FeBMb(-His), resolved recently at a high resolution (1.65 Å) (PDB entry 3MN0). The simulated structure shows that mutation of Leu to Glu at position 29 in the hydrophobic heme pocket alters the folding behavior of Mb. The hydrogen bond between Glu29 and His64 further plays a role in stabilizing the bis-His (His64/His93) coordination structure. This study offers an excellent example of using molecular modeling to gain insights in rational design of both structural and functional proteins. Proteins 2011. © 2010 Wiley-Liss, Inc.
语种：
英文
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Carbon paste electrode modified with a binuclear manganese complex as a sensitive voltammetric sensor for tryptophan
作者：
Xu, Jinsheng* ;Yuan, Yali;Li, Wei;Deng, Peihong;Deng, Jian
期刊：
Microchimica Acta ,2011年174(3-4):239-245 ISSN：0026-3672
通讯作者：
Xu, Jinsheng
作者机构：
[Deng, Peihong; Xu, Jinsheng; Li, Wei] Hengyang Normal Univ, Dept Chem & Mat Sci, Hengyang 421008, Peoples R China.;[Yuan, Yali; Deng, Jian] Nanhua Univ, Coll Chem & Chem Engn, Hengyang 421001, Peoples R China.
通讯机构：
[Xu, Jinsheng] H;Hengyang Normal Univ, Dept Chem & Mat Sci, Hengyang 421008, Peoples R China.
关键词：
Binuclear manganese(II) complex;Carbon paste electrode;Tryptophan
摘要：
We report on a carbon paste electrode that was modified with a binuclear manganese(II) complex by the drop-coating method. A study on the mechanism of the electro-oxidation of tryptophan (Trp) at this electrode indicated that it enables Trp to be determined with good sensitivity and selectivity. Second-order derivative linear sweep voltammetry at pH 4. 1 revealed that a sensitive anodic peak appears at 812 mV (vs. SCE) whose current is proportional to the concentration of Trp in the concentration range from 0. 1 to 1. 0 μmol L -1 and 1. 0 to 80 μmol L -1 , with a detection limit (S/N = 3) of 0. 08 μmol L -1 (60 s of accumulation). The method was applied to the determination of Trp in amino acid injection solutions with satisfactory results. © 2011 Springer-Verlag.
语种：
英文
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8种海藻中7种金属元素含量的测定
作者：
王晓晴;王榆元;秦珠;吴涛;曾晓雄
期刊：
光谱实验室 ,2011年28(4):1759-1762 ISSN：1004-8138
作者机构：
南京农业大学食品科技学院 南京市意卫路6号210095;南华大学化学化工学院 湖南省衡阳市常胜西路28号421001
关键词：
海藻;金属元素;原子吸收光谱法
摘要：
采用火焰原子吸收光谱法测定了谷粒马尾藻、鼠尾藻、海青菜、匍枝马尾藻、裙带菜、大叶藻、矶石鸡冠藻（红、白）这8种藻类植物中钙、镁、铁、锰、铜、锌、钾7种元素的含量。结果表明,这些海藻中含有较丰富的钙、镁、铁、钾元素,而铜、锰、锌元素含量相对较少。钙元素的含量最为丰富,铜的含量最少。该方法的加标回收率在95.7%—101.1%之间,RSD值在0.054%—1.764%之间,具有较好的准确度和精密度。本实验结果为这些藻类植物的食用,药用以及饲养价值提供了依据。
语种：
中文
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