摘要：
A defined mixed bioleaching consortium (constructed by Acidithiobacills ferrooxidans ATCC 23270, Leptospirillum ferriphilum YSK, Sulfobacillus thermosulfidooxidans ST, Acidithiobacills thiooxidans A01 and Acidithiobacills caldus S1) was used to investigate the fluoride stress response. The results showed that its growth and iron oxidation rate were obviously inhibited, while the sulphur oxidation was only barely restrained. Furthermore, the community dynamics and gene expression were assayed respectively by using real-time quantitative polymerase chain reaction assay (RT-PCR) and functional gene arrays (FGAs). From the analysis of community dynamics, the most obviously inhibited strains were S. thermosulfidooxidans ST, while L. ferriphilum YSK still maintained stable growth. After 4.8 mM of fluoride stress for 28 h, the S. thermosulfidooxidans cell concentration was decreased by about 517.8 times more than in non-stress state. From the analysis of gene expression, fluoride stress was seen to exert pleiotropic effects on different species in the consortium. The dominant species played the pivotal role in resisting fluoride stress and maintaining activities in the system, yet the inferior species also had a critical function of assisting the survival of the dominant species.
作者机构：
[胡鄂明; 史文革; 李乾; 王清良; 丁德馨] School of Nuclear Resources Engineering, University of South China, Hengyang 421001, China;[刘学端; 马丽媛] School of Minerals Processing and Bioengineering, Central South University, Changsha 410083, China;[李乾; 丁德馨] Key Discipline Lab. for National Defence of Biotechnology in Uranium Mining and Hydrometallurgy, University of South China, Hengyang 421001, China
通讯机构：
School of Minerals Processing and Bioengineering, Central South University, China
作者机构：
[李乾; 张琪; 杨敬; 胡鄂明; 张洪灿; 王清良] School of Nuclear Resource Engineering, University of South China, Hengyang 421001, Hunan, China;[张琪] Shuidong Middle School, Nayong 553308, Guizhou, China
通讯机构：
School of Nuclear Resource Engineering, University of South China, China
作者机构：
[张洪灿; 张国奇; 胡鄂明; 胡南; 王清良; 丁德馨] Key Discipline Lab for National Defense for Biotechnology in Uranium Mining and Hydrometallurgy, University of South China, Hengyang 421001, China;[蒋晓辉; 阳奕汉] Tianshan Uranium Corporation, China National Nuclear Corporation, Yining 835000, China;[王清良] School of Minerals Processing and Bioengineering, Central South University, Changsha 410083, China
通讯机构：
Key Discipline Lab for National Defense for Biotechnology in Uranium Mining and Hydrometallurgy, University of South China, China
作者机构：
[余润兰; 邱冠周] School of Minerals Processing and Bioengineering, Central South University, Changsha 410083, Hunan, China;[杨懿全; 杨敬; 陈勇博; 胡鄂明; 张洪灿] School of Nuclear Resource and Nuclear Fuel Engineering, University of South China, Hengyang 421001, Hunan, China;[王清良] School of Minerals Processing and Bioengineering, Central South University, Changsha 410083, Hunan, China<&wdkj&>School of Nuclear Resource and Nuclear Fuel Engineering, University of South China, Hengyang 421001, Hunan, China
通讯机构：
School of Minerals Processing and Bioengineering, Central South University, China
作者机构：
[Wang Qing-liang; Yu Run-lan; Qiu Guan-zhou] Cent S Univ, Sch Minerals Proc & Bioengn, Changsha 410083, Peoples R China.;[Ding De-xin; Wang Qing-liang; Hu E-ming] Univ S China, Key Discipline Lab Natl Def Biotechnol Uranium Mi, Hengyang 421001, Peoples R China.
通讯机构：
[Wang Qing-liang] C;Cent S Univ, Sch Minerals Proc & Bioengn, Changsha 410083, Peoples R China.
会议名称：
24th International Mineral Processing Congress (IMPC)
会议时间：
SEP 24-28, 2008
会议地点：
Beijing, PEOPLES R CHINA
会议主办单位：
[Wang Qing-liang;Yu Run-lan;Qiu Guan-zhou] Cent S Univ, Sch Minerals Proc & Bioengn, Changsha 410083, Peoples R China.^[Wang Qing-liang;Ding De-xin;Hu E-ming] Univ S China, Key Discipline Lab Natl Def Biotechnol Uranium Mi, Hengyang 421001, Peoples R China.
关键词：
sulfate reducing bacteria;in-situ leaching of uranium;radioactively contaminated groundwater;bioremediation
摘要：
In the case of in-situ leaching of uranium, the primitive geochemical environment for groundwater is changed since leachant is injected into the water bearing uranium deposit. This increases the concentration of SO_4~(2-), uranium and other heavy metal ions and results in the groundwater contamination. The effects of pH values of the simulated solution on the reduction of SO_4~(2-) and the removal of uranium and other heavy metal ions by sulfate reducing bacteria(SRB) were studied. The results show that, when the pH value of the simulated solution is about 8, the reduction rate of SO_4~(2-) by SRB and the removal rate of uranium, Mn~(2+), Zn~(2+), Pb~(2+) and Fe~(2+) will reach their highest values. A bioremediation technique for remediation of groundwater in in-situ leaching uranium mine can be developed.
