作者机构：
[Zhao Feijun; Zeng Tiebing; Yu Jian; Chen Xi; Wu Yimou] Pathogenic Biology Institute,University of South China,Hengyang City,Hunan Province 421001,China;[Liu Shuangquan] Department of Anatomy & Neurobiology,Xiangya School of Medicine,Central South University,Changsha,410013,China;[Liu Shuangquan] First Affiliated Hospital,University of South China,Hengyang City,Hunan Province 411000,China;[Zhang Xiaohong] Department of Histology and Embryology,School of Medicine,University of South China,Hengyang City,Hunan Province 421001,China;[Gu Weiming] Skin Diseases and STD Hospital,Shanghai 200050,China
通讯机构：
[Yang, Luoyan] C;Cent S Univ, Xiangya Hosp 2, Dept Urol, Changsha 410011, Hunan, Peoples R China.
关键词：
beta-elemene;survivin;Bcl-xL;Mta-1;human bladder cancer
摘要：
β-elemene, a non-cellular antineoplastic agent, may be used to effectively inhibit the growth and proliferation of various types of tumor cells by inhibiting the nucleic acid synthesis or inducing their apoptosis and differentiation. The aim of this study was to investigate the expression, as well as the effects, of Mta-1, survivin and Bcl-xL in T24 bladder cancer cells following β-elemene treatment. The expression of the three proteins in T24 cells following β-elemene treatment was analyzed by immunocytochemistry staining and western blot analysis. The survival rate and apoptosis of T24 cells following β-elemene treatment was detected by MTT assay and TUNEL staining. We analyzed the internal corelations between apoptosis-associated genes, tumor metastasis-associated genes (cancer genes) and cell apoptosis, and investigated the mechanism of action by which β-elemene induces the apoptosis of T24 cells at a molecular level. These results provide scientific evidence for further study on the anticancer effect of β-elemene in carcinoma of the urinary bladder. In this study, it is shown that β-elemene downregulates the expression of survivin, Bcl-xL and Mta-1 in tumor cells. The apoptosis of T24 cells is dependent on the dosage and length of incubation time of β-elemene.
期刊：
Journal of Neuroimaging,2010年20(3):234-239 ISSN：1051-2284
通讯作者：
Wong, Ka Sing
作者机构：
[You, Yong] Nanhua Univ, Affiliated Hosp 1, Dept Neurol, Hengyang, Hunan, Peoples R China.;[Lau, Alex; Mok, Vincent; Leung, Thomas; Leung, Howan; Chen, Xiangyan; Hao, Qing; Wong, Ka Sing] Chinese Univ Hong Kong, Dept Med & Therapeut, Shatin, Hong Kong, Peoples R China.;[Lau, Alex; Mok, Vincent; Leung, Thomas; Leung, Howan; Chen, Xiangyan; Hao, Qing; Wong, Ka Sing] Chinese Univ Hong Kong, Dept Diagnost Radiol & Organ Imaging, Shatin, Hong Kong, Peoples R China.;[Wong, Ka Sing] Chinese Univ Hong Kong, Prince Wales Hosp, Dept Med & Therapeut, Shatin, Hong Kong, Peoples R China.
通讯机构：
[Wong, Ka Sing] C;Chinese Univ Hong Kong, Prince Wales Hosp, Dept Med & Therapeut, Shatin, Hong Kong, Peoples R China.
作者机构：
[Chen Lin; Yu Min-jun; Wan Yan-ping; Zhu Cui-ming; Cao Qing-xiang; Liu An-yuan; Chan Xi] Univ S China, Inst Pathogen Biol, Hengyang 421001, Peoples R China.
通讯机构：
[Wan Yan-ping] U;Univ S China, Inst Pathogen Biol, Hengyang 421001, Peoples R China.
关键词：
human cytomegalovirus ( HCMV);IE1;macrophages;IL-1 beta;TNF-alpha;apoptosis
摘要：
Objective: To investigate the effect of human cytomegalovirus (HCMV) IE1 protein on the secretory activity and apoptosis of macrophages. Methods: The eukaryotic expression vector pEGFP-C1/IE1 was used to transfect THP-1-macrophages. 48 h after transfection, the expression and localization of GFP or GFP-IE1 was observed under fluorescent microscope. The levels of IL-1β and TNF-α in the culture media were examined by ELISA, and the mRNA expression of them was analyzed by RT-PCR. Cell undergoing apoptosis were determined by flow cytometry using the propidium iodide (PI) staining method. The data were analyzed by SPSS 13.0. Results: As observed under fluorescent microscope, the expressions of GFP-IE1 and GFP by plasmid pEGFP-C1/IE1 or pEGFP-C1 in THP-1-macrophages could be found in nuclei or whole cells. Conclusion: As demonstrated by RT-PCR and ELISA, mRNA and protein expressions of IL-1β and TNF-α and promotes apoptosis in THP-1-macrophages.
