期刊论文

Wan Yan-ping

英文

中国癌症研究：英文版

1000-9604

2008

20

1

12-16

10.1007/s11670-008-0012-5

This work was supported by the Education Committee of Hunan Province (No. 05C472). *Author to whom correspondence should be addressed. E-mail: yanpingwan@hotmail.com **E-mail: chenlin_0050@yahoo.com.cn

本校为第一且通讯机构

Objective: To investigate the effect of human cytomegalovirus (HCMV) IE1 protein on the secretory activity and apoptosis of macrophages. Methods: The eukaryotic expression vector pEGFP-C1/IE1 was used to transfect THP-1-macrophages. 48 h after transfection, the expression and localization of GFP or GFP-IE1 was observed under fluorescent microscope. The levels of IL-1β and TNF-α in the culture media were examined by ELISA, and the mRNA expression of them was analyzed by RT-PCR. Cell undergoing apoptosis were determined by flow cytometry using ...

Objective:To investigate the effect of human cytomegalovirus（HCMV）IE1 protein on the secretory activity andapoptosis ofmacrophages.Methods:Theeukaryotic expression vector pEGFP-C1/IE1 was used totransfect THP-1-macrophages.48 h after transfection,the expression and localization of GFP or GFP-IE1 was observed under fluorescent microscope.The levels of IL-1βand TNF-αin the culture media were examined by ELISA,and the mRNA expression of them was analyzed by RT-PCR.Cell undergoing apoptosis were determined by flow cytometry using the propidium ...