Attachments of Acidithiobacillus ferrooxidans ATCC 23270 onto elemental sulfur, quartz and complex chalcopyrite were investigated by analysis of its extracellular polymeric substances as well as applying Langmuir and Freundlich equations. The two equations fitted the adsorption equilibrium data with significant correlation coefficient over 0.9. This indicated that bacterial attachment is complicated and involves Langmuir and Freundlich characterizations. Sulfur-grown cells showed the highest affinity for the three solid substrates. The investigated complex chalcopyrite possessed a higher maximum adsorption capacity for A. ferrooxidans than elemental sulfur or quartz. The Freundlich fitting parameters suggested that quartz had a weaker adsorption capacity and smaller adsorption areas than elemental sulfur or the complex chalcopyrite. It is not the content of total carbohydrates or proteins in EPS but their ratios that determine the affinity differences between cells and substrates.
[王晓亮; 彭国文] School of Chemistry and Chemical Engineering, University of South China, Hengyang, China;[杨锦然; 黄红; 周归] Wang Chuanshan College, University of South China, Hengyang, China;[彭国文] School of Resources and Safety Engineering, Central South University, Changsha, China;[胡南; 肖方竹; 彭国文; 丁德馨; 张志军] Key Discipline Laboratory for National Defence for Biotechnology in Uranium Mining and Hydrometallurgy, University of South China, Hengyang, China
School of Chemistry and Chemical Engineering, University of South China, Hengyang, China