期刊：
MOLECULAR MEDICINE REPORTS,2012年5(3):753-760 ISSN：1791-2997
通讯作者：
Wang, Xiaochun
作者机构：
[Zhou, Feng; Liu, Lipeng; Tian, Zhi; Su, Min; Huang, Chunxia; Liu, Meiling; Guo, Xiaofang; Long, Yu] Changsha Med Univ, Dept Biochem & Mol Biol, Changsha 410219, Hunan, Peoples R China.;[Wang, Xiaochun] Cent S Univ, Dept Med Lab Examinat, Changsha 410013, Hunan, Peoples R China.;[Yu, Xiaohua] Nanhua Univ, Inst Pharm & Pharmacol, Hengyang 421001, Hunan, Peoples R China.;[Wu, Xinhua] Cent S Univ, Xiangya Hosp, Dept Obstet & Gynecol, Changsha 410078, Hunan, Peoples R China.
通讯机构：
[Wang, Xiaochun] C;Cent S Univ, Dept Med Lab Examinat, Changsha 410013, Hunan, Peoples R China.
关键词：
Apoptosis;Bcl-2;HeLa;MiR-143;Proliferation
摘要：
microRNAs (miRNAs) are small non-coding RNA molecules of 21-24 nt that regulate the expression of other genes by transcriptional inhibition or translational repression. Multiple lines of evidence suggest that miRNAs play important roles in tumor development and progression. We identified 24 mi RNAs markedly and aberrantly expressed in human cervical cancer. The most significantly deregulated was miR-143 as determined by miRNA microarray analysis. miR-143 was introduced into He La cells and it was found that the overexpression of miR-143 significantly inhibited He La cell proliferation and promoted apoptosis; anti-miR-143 rescued the effects. He La cells transfected with pre-miR-143, pre-anti-miR-143 or control mi RNA precursor were injected subcutaneously into the flanks of female athymic nude mice, and the overexpression of miR-143 suppressed the formation of tumors. Compared with normal cervical tissues, the levels of Bcl-2 were increased in miR-143-downregulated tissues. Sustained overexpression of miR-143 in He La cells resulted in suppression of Bcl-2 expression, and knockdown of miR-143 by anti-miR-143 increased Bcl-2 expression. In addition, overexpression of Bcl-2 partially reversed the inhibition of proliferation and promotion of apoptosis in the He La cells caused by miR-143. Furthermore, miR-143 suppressed the activity of a luciferase reporter carrying the 3'-UTR of Bcl-2, which was abolished by mutation of the predicted miR-143-binding site, indicating that Bcl-2 is a miR-143 target gene. Our study revealed a molecular link between miR-143 and Bcl-2. Direct involvement in the regulation of Bcl-2 may be one of the mechanisms through which mi R-143 may play a role in the pathogenesis of cervical cancer.
期刊：
Applied Mechanics and Materials,2012年204-208:3776-3781 ISSN：1660-9336
通讯作者：
Wu, Xianlei
作者机构：
[Shi, Jianjun; Shan, Zhi; Wu, Xianlei] Univ South China, Sch Urban Construct, Hengyang 421001, Peoples R China.
通讯机构：
[Wu, Xianlei] U;Univ South China, Sch Urban Construct, Hengyang 421001, Peoples R China.
会议名称：
International Conference on Civil, Architectural and Hydraulic Engineering (ICCAHE 2012)
会议时间：
AUG 10-12, 2012
会议地点：
Zhangjiajie, PEOPLES R CHINA
会议主办单位：
[Wu, Xianlei;Shi, Jianjun;Shan, Zhi] Univ South China, Sch Urban Construct, Hengyang 421001, Peoples R China.
会议论文集名称：
Applied Mechanics and Materials
关键词：
MATLAB image process technique;Rheological constants;SCC;V-Funnel
摘要：
This template is mainly about the analysis of mechanical property of SCC in a flow state in the V-Funnel. It aims to establish the testing formula of rheological constants by Engineering Fluid Mechanics, which can provide academic basis for V-Funnel test of SCC properties. MATLAB image process technique was introduced in the process which can greatly improve its precision and reliability. It can also provide a new train of thought to experimental data.
