作者机构：
[Peng, K.; Sun, Y. H.; Sun, W. Q.; Dong, B. H.; Jin, H. Y.; Tang, Y. L.; Yang, Y. Z.; Ye, X.] Nanhua Univ, Inst Cardiovasc Dis, Hengyang, Peoples R China.
摘要：
The title compound (C18H15FN6OS) has been synthesized and its crystal structure was determined by X-ray analysis. The crystal belongs to monoclinic system, space group P21/n with a = 13.983(10), b = 7.337(5), c = 16.702(12) (A), α = 90, β = 98.277(12), γ = 90°, V = 1696(2)(A)3, Z = 4, Dc= 1.498 g/cm3, Mr = 382.42,μ = 0.224 mm-1, F(000) = 792, the final R = 0.0362 and wR = 0.0878 for 2579 observed reflections with I ＞ 2σ(Ⅰ). In the title compound, the atoms of C(1),C(2), C(3), C(4), C(5), N(1), N(2), N(3), N(4), N(5), N(6), O(1) and S(1) form a fully delocalized system with the average deviation of 0.0307(A). There exists a part of electron delocalization over the planes of Ⅰ (pyrazolo[3,4-d]pyrimidin-4-one moiety), Ⅱ (phenyl moiety) and Ⅲ (p-fluorophenylamine moiety). In the molecule, intermolecular hydrogen bonds between N(6)-H(6)…O(1)and C( 15)-H( 15)…O( 1 ) are observed.
作者机构：
[Fu, MD] Sichuan Univ, W China Sch Preclin & Forens Med, Apolipoprot Res Unit, Dept Biochem & Mol Biol, Chengdu 610041, Peoples R China.;Nanhua Univ, Dept Biochem & Mol Biol, Hengyang 421001, Hunan, Peoples R China.;Hoist Grp Postdoctoral Work Stn, Chengdu 610075, Sichuan, Peoples R China.
通讯机构：
[Fu, MD] S;Sichuan Univ, W China Sch Preclin & Forens Med, Apolipoprot Res Unit, Dept Biochem & Mol Biol, Chengdu 610041, Peoples R China.
关键词：
Gene polymorphism;HDL subclasses;Hyperlipidemia;Lipoprotein lipase;Two-dimensional gel electrophoresis-immunodetection
摘要：
Background: Different high-density lipoprotein (HDL) subclasses have distinct but interrelated metabolic functions. HDL directly influences the atherogenic process, and changes in HDL subclasses distribution may be related to the incidence and prevalence of atherosclerosis. Lipoprotein lipase (LPL) is an important enzyme for hydrolysis of triglyceride-rich lipoproteins, and its activity is positively correlated with the plasma HDL cholesterol level. LPL gene HindIII polymorphism has been found associated with variations in lipid levels, but the impact on HDL subclasses distribution is less clearly established. Methods: The relative apolipoprotein (apo) A-I contents (% apoA-I) of plasma HDL subclasses were determined by two-dimensional gel electrophoresis coupled with immunodetection and LPL gene HindIII polymorphism was assayed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) in 173 hyperlipidemic and 155 normolipidemic subjects. Results: The frequencies of 495TT genotype and allele T were the highest both in the hyperlipidemic and control groups. Compared with the control group, the frequency of 495TT genotype was higher, while the frequencies of 495TG and 495GG genotypes were significantly lower (P < 0.05) in the hyperlipidemic group. Two-dimensional gel electrophoresis and immunodetection showed that HDL subclasses distribution was altered in hyperlipidemia, and had a general shift toward smaller size. Compared with the control group, the hyperlipidemic group had significantly higher relative apoA-I contents of pre beta(1)-HDL, pre beta(2)-HDL, HDL3b and HDL3(a) (P < 0.05) and lower HDL2a and HDL2b levels (P < 0.001). In the hyperlipidernic group, allele T carriers' frequency was higher than that in the control group (P < 0.05), and the genotype of 495TT showed higher levels of plasma TG, apoB100, TG/HDL-C ratio, relative apoA-I contents of pre beta(1)-HDL, HDL3b and lower HDL2a, HDL2b compared with that of the 495GG genotype subgroup (P < 0.05). In the control group, the genotype of 495TT had higher plasma TG, HDL3c and lower HDL2a compared with that of 495GG subgroup (P < 0.05). Conclusions: The 495TT genotype of LPL gene HindIII polymorphism was associated with changes of HDL subclasses distribution in Chinese population with hyperlipidemia. The particle size of HDL shifted toward smaller, which, in turn, indicated that RCT might be weakened and HDL maturation might be abnormal in hyperlipidemic subjects with 495TT genotype. (c) 2005 Elsevier B.V. All rights reserved.
摘要：
To investigate the changes in drug sensitivity of Bcl-2 siRNA transfected HepG2 cells. Bcl-2 siRNA and negative siRNA expression vector were constructed and stably transfected into HepG2 cells. RT-PCR and Immunofluorescence were used to detect the target gene expression. Western Blotting was used to detect Bcl-2, Bax and caspase-3 protein expressiom. Drug sensitivity of the cells to 5-fluorouracil (5-FU) and 10-hydroxycamptothecin (HCPT) were analyzed with MTT and flow cytometry. Results were following: (1) the mRNA and protein expression level of Bcl-2 in Bcl-2 siRNA stable transfectants were reduced compared with negative siRNA transfected or untreated cells. Accordingly, Bax protein expression had no change and caspase-3 protein expression showed significantly be up regulated; (2) MTT results showed that Bcl-2 siRNA transfectants had higher cell inhibitory rates after treated with 5-FU or HCPT; (3) flow cytometry results demonstrated that sub G1 population increased in Bcl-2 siRNA transfected cells compared with negative siRNA or untreated cells. After addition 5-FU (1300 mg/l) and HCPT (0.72 mg/l), Bcl-2 siRNA cells showed higher sub G1 population than negative siRNA or untreated cells. siRNA targeting Bcl-2 gene can specifically down-regulate Bcl-2 expression, increased Bax/Bcl-2 ratio expression and caspase-3 activity in HepG2 cells, which lead to increase cells spontaneous apoptosis and sensitize cells to 5-FU or HCPT. Bcl-2 siRNA may be a potential therapy agent against human hepatoblastoma.
摘要：
In this paper, the basic definitions of inner-product and outer-product of Vague set are introduced. Based on that, some characters of them are brought forward and the particular proof are presented afterward.
作者机构：
[Ding De-xin] Cent S Univ, Sch Resources & Safety Engn, Changsha 410083, Peoples R China.;Nanhua Univ, Sch Architectural Resources & Environm Engn, Hengyang 421001, Peoples R China.
通讯机构：
[Ding De-xin] C;Cent S Univ, Sch Resources & Safety Engn, Changsha 410083, Peoples R China.
关键词：
mining induced surface subsidence;fuzziness and interaction of parameters;artificial neural fuzzy inference system
摘要：
There are many parameters influencing mining induced surface subsidence. These parameters usually interact with one another and some of them have the characteristic of fuzziness. Current approaches to predicting the subsidence cannot take into account of such interactions and fuzziness. In order to overcome this disadvantage, many mining induced surface subsidence cases were accumulated, and an artificial neuro fuzzy inference system(ANFIS) was used to set up 4 ANFIS models to predict the rise angle, dip angle, center angle and the maximum subsidence, respectively. The fitting and generalization prediction capabilities of the models were tested. The test results show that the models have very good fitting and generalization prediction capabilities and the approach can be applied to predict the mining induced surface subsidence.
期刊：
Applied Mathematics and Computation,2006年181(2):803-815 ISSN：0096-3003
通讯作者：
Chen, FD
作者机构：
[Chen, Fengde] College of Mathematics and Computer Science, Fuzhou University, Fuzhou, Fujian 350002, China;[Huang, Zhenkun] School of Sciences, Jimei University, Xiamen, Fujian 361021, China;[Liao, Xinyuan] Department of Mathematics, Shanghai University, Shanghai, 200444, China;[Liao, Xinyuan] School of Mathematics and Physics, Nanhua University, Hengyang, Hunan 421001, China
通讯机构：
[Chen, FD ] ;Fuzhou Univ, Coll Math & Comp Sci, Fujian 350002, Peoples R China.
