期刊论文

中文

长沙医学院学报

2013

2

7-10

(编号:09C142):湖南省教育厅科研基金资助项目

本校为第一机构

目的探讨茶多酚对甲醛致人内皮细胞氧化损伤的保护作用。方法采用体外常规培养内皮细胞,取对数生长期的细胞进行实验。实验设对照组、甲醛损伤组和低、中、高剂量茶多酚保护组,其中茶多酚先与细胞预孵1 2h后加入0.1 mmoL/L甲醛,置CO2培养箱培养4h,检测细胞培养液中SOD活力及LDH、MDA、N0的含量,s P法检测NF-KB。结果茶多酚各保护组与甲醛损伤组比较SOD活力明显升高,LDH、MDA含量均降低(P【0.05);茶多酚各保护组内皮细胞N0释放量减少、NF—KB表达明显下降与甲醛损伤组比较均有显著性(P【0.05)。结论茶多酚可提高内皮细胞抗氧化酶的活性,减轻甲醛所致的氧化损伤;茶多酚通过抑制NF—KB的表达,减少N...

Objective To investigate the protective effect of tea polyphenols on oxidative damaged endothelial cells induced by Formaldehyde.Methods Human umbilical vein endothelial cells(HUVEC-12)were cultured with DMEM in vitro and the cells in the exponential phase of growth to experiment were selected.They were divided into nomal cells group formaldehyde-induced injury group(0.1 mmol/L),low,medium and high doses of Tea Polyphenols protection group.The cells were pre-incubated with tea polyphenols,after 12h.adding 0.1 mmol/L formaldehyde and put in C02 Incubators foster home for 4h,then the SOD activit...