Primerless Amplification for Circulating Tumor DNA Assays

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作者信息关键词期刊信息基础信息归属信息摘要

成果类型：

期刊论文

作者：

Xiao, Li;Luo, Di-Xian*;Pu, Wangyang;Luo, Weihao;Pan, Yunzhi;...

通讯作者：

Luo, Di-Xian;Zhang, Jia;Li, Kai;He, Nongyue

作者机构：

[Xing, Chungen; Pu, Wangyang; Liu, Xuan; Zhang, Rong; Xiao, Li] Soochow Univ, Lab Mol Med, Affiliated Hosp 2, Suzhou 215004, Peoples R China.

[Li, Kai; Luo, Weihao; Li, Wen; Luo, DX; Zhang, Jia; Li, K; Sirois, Pierre; Luo, Di-Xian] Univ South China, Natl & Local Joint Engn Lab High Mol Diag Technol, Translat Med Inst, Peoples Hosp Chenzhou 1, Chenzhou 423000, Peoples R China.

[Pan, Yunzhi] Soochow Univ, Affiliated Infect Dis Hosp, Suzhou 215007, Peoples R China.

[Xu, Huifen] Zhejiang Univ, Sch Med, Childrens Hosp, Hangzhou 310052, Zhejiang, Peoples R China.

[Wang, Han; Sun, Yi] Soochow Univ, Ctr Circadian Clock, Sch Biol & Basic Med Sci, Med Coll, Suzhou 215123, Peoples R China.

通讯机构：

[Luo, DX; Zhang, J; Li, K] U

[He, Nongyue] S

Univ South China, Natl & Local Joint Engn Lab High Mol Diag Technol, Translat Med Inst, Peoples Hosp Chenzhou 1, Chenzhou 423000, Peoples R China.

Southeast Univ, State Key Lab Bioelect, Nanjing 210096, Jiangsu, Peoples R China.

语种：

英文

关键词：

APC;CANCER DIAGNOSIS;CTDNA;EGFR;GENOTYPE SWITCH;PIK3CA

期刊：

JOURNAL OF BIOMEDICAL NANOTECHNOLOGY

ISSN：

1550-7033

年：

2019

卷：

期：

页码：

1052-1060

DOI：

10.1166/jbn.2019.2746

基金类别：

National Key Basic Research Program of the PRCNational Basic Research Program of China [2014CB744504]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81120108013, 81271922, 81301267, 81372825, 81530054]; Hunan Province Science Fund for Distinguished Young Scholars [2018JJ1021]; Jiangsu Province's program [BL2013016, QN-868]; Key R&D Program of Hunan Province [2017SK2172]; Natural Science Foundation of Hunan ProvinceNatural Science Foundation of Hunan Province [2016JJ2114, 2017JJ2003, 2017JJ2004]; Hunan Province Technological Innovation Guiding Plan, Clinical Medical Technology Innovation Guidance Project [2017SK51101]; Health Department project of the Hunan Province [B2017182, B20180378, B20180266, C2019003]; Suzhou's Program [SZS201618, SYS201727]; Science and Technique Foundation of Chenzhou [jsyf2017021, jsyf2017023]

机构署名：

本校为通讯机构

院系归属：

医学院

摘要：

A primerless amplification suitable for enrichment of particular bygenotype Ingenta cfDNA which is a one-dimensional material has been developed. This primerless amplification coordinated by two thermostable enzymes of endonuclease and proofreading polymerase, functions as a genotype switch in analyzing cfDNA. The endonuclease digests the wild-typed fragments into mega-primer and discriminately destroys the wild-type DNA alleles. The DNA polymerase proofreads the mega-primer and then extends the mega-primer using the mutant DNA as the template....

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Primerless Amplification for Circulating Tumor DNA Assays

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