期刊论文

Purification of ScuteUarin by Column Chromatography

中文

中国医药指南

1671-8194

2012

10

7

2,12

10.3969/j.issn.1671-8194.2012.07.002

湖南省中医药管理局（课题编号:2010089）资助；

本校为第一机构

目的 优选灯盏乙素的柱层析纯化条件.方法 采用高效液相色谱法检测纯度,考察了不同固定相和流动相对灯盏乙素分离效果的影响.结果 灯盏乙素的最佳纯化工艺条件为:固定相200~300 目的 硅胶柱,流动相V(CH2Cl2):V(CH3OH)=5:1,常压洗脱.结论 该方法操作简单,分离效果好,使用成本低.

To optimize the column chromatography methods to sublimate the scutellarin. Methods The conditions of column chromatography were studied by investigating into the purity and yield rates of scutellarin. A high performance liquid chromatography assay was used for measurements. Results The optimal operation conditions of column chromatography separation were： the 200-300 mesh silica gel can be chosen as the stationary phase, the mixture ofCH_2CI_2 ： CH3OH =5 ： 1 （by volume） is the suitable mobile phase. Conclusion The re...