期刊论文

中文

中外医药研究

1007-1180

2014

20

9

130-131

10.3969/j.issn.1009-4393.2014.3.088

国家自然科学基金项目（31200140）；

本校为第一机构

目的：探讨兰索拉唑对幽门螺杆菌（helicobacter pylori，Hp）感染的胃上皮细胞的体外抗炎效应。方法体外培养胃上皮细胞系AGS，加入兰索拉唑和培养的Hp感染不同时间。ELISA检测IL-1β和IL-8的产生，Western blot检测NF-κB p 65亚基的核转位以及红素氧合酶1（Heme oxygenase-1， HO-1）蛋白表达水平。结果兰索拉唑孵育8 h后，能显著抑制Hp诱导的IL-1β和IL-8。同时，兰索拉唑能抑制NF-κB p 65亚基的核转位，并能上调HO-1蛋白的表达。结论兰索拉唑可能通过抑制NF-κB的激活，上调HO-1的表达而发挥对胃上皮细胞的抗炎作用。

Objective To investigated the anti-inflammatory effects of Lansoprazole in H. pylori-infected gastric epithelial cells in vitro. Method Gastric epithelial cell line AGS was cultured in vitro, and was incubated with Lansoprazole and H. pylori for various times. Production of IL-6 and IL-8 were detected by ELISA, activation of NF-κB and expression of Heme oxygenase-1 was detected by Western blot. Results After incubated for 8 h, Lansoprazole could significantly inhibit H. pylori induced IL-1βand IL-8 production. And Lansoprazole could also indu...