期刊论文

Xiao, JF;Zhang, Hong-ming;Lü, B

中文

光谱学与光谱分析

1000-0593

2025

45

6

1566-1577

10.3964/j.issn.1000-0593(2025)06-1566-12

国家自然科学基金项目(32372357))； 湖南省教育厅科研项目(21B0439)资助；

本校为第一且通讯机构

在发酵过程的近红外在线检测中，由于发酵液中需要持续通入氧气来促进微生物的生长和代谢活动，常会在发酵液中产生气泡。发酵液中的气泡经过探头前方时，会对近红外光谱的强度产生较大干扰。为了剔除发酵液近红外在线检测过程中采集到的气泡引起的异常光谱，减少光谱波动，提出了一种正态分布筛选方法。制备了600 g质量分数为10%的葡萄糖溶液，每隔30 s加2 g葡萄糖溶液至盛有600 mL蒸馏水的反应釜中，搅拌均匀，然后计算和记录反应釜内葡萄糖溶液的质量分数，并在反应釜底部通入氧气产生气泡，利用近红外光谱仪采集反应釜内葡萄糖溶液的近红外光谱。分别采用主成分分析（principal component a...

In the near-infrared online detection of the fermentation process, bubbles are often generated in the fermentation broth due to the need to continuously pass oxygen into the fermentation broth to promote microbial growth and metabolic activities, When the bubbles in the fermentation broth pass in front of the probe, they will interfere with the intensity of the near-infrared (NIR) spectrum, To eliminate the abnormal spectra caused by bubbles collected during the near-infrared online detection of fermentation broth and reduce spectral fluctuations, a normal distribution screening method is prop...