期刊论文

Guo Ping

中文

光谱学与光谱分析

1000-0593

2010

30

6

1511-1515

10.3964/j.issn.1000-0593(2010)06-1511-05

国家自然科学基金项目（30371626） 湖南省科技厅项目（2008FJ3084）资助

本校为第一且通讯机构

为了给死亡时间推断的研究提供新的途径与数据, 探讨了人死亡后组织细胞DNA含量变化与死后时间间隔关系. 将离体肾、肝组织置于特定环境中, 在不同时间点提取离体组织, 利用激光共焦显微拉曼光谱技术和统计学方法, 在拉曼位移测试范围内, 对死后不同时间肾、肝组织DNA含量变化进行拉曼光谱检测与统计学分析. 结果显示:人死亡后48~72 h, 随着死亡时间的推移, 肾、肝组织Raman光谱的主要散射峰峰位没有明显变化, 而其峰高有明显差异;所测组织的激光拉曼光谱特征峰的相对峰强值(I_(1094)/I_(2923))随死亡时间的推移而减小. 因此可以得出结论:人死亡后肾、肝组织细胞DNA含量随死亡时间延长呈下降趋势...

The present work is to study the relationship between DNA degradation in tissue cells and estimation of postmortem interval, and to provide a new way for the estimation of postmortem interval(PMI). The tissue cells of kidney and liver in vitro obtained at different time points were kept in a controlled environment. The kidney tissue and liver tissue were taken out every 4 h, during 48-72 h after death at 25°C. The DNA content in different tissue cells was examined by means of confocal Raman micro spectroscopy with an excitation wavelength of 5...