期刊论文

Deng, J

中文

光谱学与光谱分析

1000-0593

2006

26

3

526-528

10.3321/j.issn:1000-0593.2006.03.036

国家自然科学基金委重点课题子课题（20135010）资助项目.

本校为第一且通讯机构

对苯酚、2，4-二氯苯酚、2，6-二氯苯酚及间-苯二酚等四种苯原酚化合物作为过氧化物酶（POD）催化4-氨基安替比林（4-AA）-过氧化氢-酚显色体系测定血糖的葡萄糖氧化酶法（GOI）-Trinder）进行比较研究。结果表明，苯酚、2，4-二氯苯酚及间-苯二酚三种苯原酚分别构成GOD-POD-4-AA-H2O2-酚显色体系测定血糖时，其检测结果均无显著性差异（P〉O．05），但2，4-二氯苯酚显色体系的显色灵敏度、方法的精密度和准确度均优于苯酚显色体系。间-苯二酚显色体系灵敏度不及苯酚，但检测速度快，线性范围宽，可用于较高浓度葡萄糖溶液的在线快速分析。

Comparative studies were carried out on GOD-trinder method for measuring glucose in serum with four different kinds of benzene-original phenols-phenyl hydroxide, 2,4-dichlorophenol, 2,6-dichlorophenol and resorcinol. It is shown that when the three coloring systems-phenyl hydroxide, 2,4-dichlorophenol or resorcinol coupling with glucose oxidase-peroxidase-4-a minoan-tipyrine-hydrogen peroxide (GOD-POD-4-AA-H2O2) respectively were used for clinical assay of glucose in serum, the determination results showed no significant differences (P>0.05). Compared with phenyl hydroxide, the coloring sys...