期刊论文

Liao, LF

中文

光谱学与光谱分析

1000-0593

2002

22

4

659-661

10.3321/j.issn:1000-0593.2002.04.037

湖南省卫生厅科研项目

本校为第一且通讯机构

硝基酪氨酸中的硝基可还原为氨基再重氮化后被碘定量取代,利用碘-亚硝酸-亚砷酸体系催化光度法可对取代硝基的碘进行测定.据此建立了碘取代硝基偶合催化光度法测定痕量硝基酪氨酸的新方法.本方法的检出限为0.002 μmol*L-1 ,线性范围为0.005～0.25 μmol*L-1 .应用该法检测了过亚硝酸根与酪氨酸反应后的产物中硝基酪氨酸的含量,获得了满意的结果.

The nitroxyl of nitrotyrosine can be substituted quantitatively by iodide when the nitroxyl is reduced to amino and then diazotized to diazonium. The iodide can be dertermined by catalytic spectrophotometric method with iodide-nitrous acid-arsenious acid system.Thus, a new catalytic spectrophotometric method combining the substitution of nitroxyl by iodide for the determination of nitrotyrosine has been established. The limit of detection is 0.002 μmol L-1 and the linear range is 0.005 - 0.25 μmol L-1. The method has been used for the determi...