会议论文

The Role of Plant Homeodomain Finger-2 in Oxidized Low Density Lipoprotein-induced Activation of Nuclear Factor-kappa B in THP-1 Macrophages

中文

2018

780-784

第十二次全国动脉硬化性疾病学术会议

第十二次全国动脉硬化性疾病学术会议论文集

20131011

江苏苏州

中国病理生理学会<&wdkj&>国际动脉粥样硬化学会中国分会<&wdkj&>中国动脉硬化杂志编辑部

本校为第一机构

目的：动脉粥样硬化(atherosclerosis ,As)是一种炎症疾病，多种炎症因子参与了As的发生发展川。低密度脂蛋白(low density lipoprotein, LDL)是促进As进展的主要危险因素。观察氧化型低密度脂蛋白对THP-1巨噬细胞植物同源结构域指蛋白2(PHF-2)表达的影响,探讨表观遗传调节在氧化型低密度脂蛋白诱导巨噬细胞炎症反应中的作用. 方法：用不同浓度(0、50、100、150mg/L)氧化型低密度脂蛋白处理THP-1巨噬细胞4h,采用酶联免疫吸附法检测肿瘤坏死因子α和巨噬细胞炎性蛋白1β的表达;采用实时荧光定量聚合酶链反应和Western blot分别检测PHF-2mRNA和蛋白质的表达;采用免疫共沉淀法检测PHF-2和p65核因...

　　Aim To investigate the effect of oxidized low density lipoprotein (ox-LDL) on the expression of plant homeodomain finger-2 (PHF-2) and explore the potential effect of epigenetic regulation on ox-LDL-induced inflammatory response in macrophages.Methods THP-1 macrophages were exposed to ox-LDL with different levels (0, 50, 100, 150mg/L) for 4 h.Enzyme linked immunosorbent assay was used to determine levels of tumor necrosis factor-α (TNF-α) and macrophage inflammatory protein-1β (MIP-1β).Red-time quantitative polymerase chain reaction (Re...