期刊论文

Influence of HBV on the interleukin-17 receptor signaling pathway in HepG2 cells

中文

世界华人消化杂志

1009-3079

2012

20

16

1389-1395

湖南省研究生科研创新项目基金资助项目,No.CX2010B382 湖南省教育厅青年项目基金资助项目,No.11B110

本校为第一机构

目的:研究乙型肝炎病毒(hepatitis B virus,HBV)重组腺病毒对HepG2细胞的IL-17R和接头蛋白Actl表达的影响,以及HBV对IL-17诱导NF-kB活化的影响.方法:采用实时荧光定量PCR(real-time PCR)检测HepG2细胞的IL-17、IL-17R和Actl的mRNA表达;蛋白免疫印迹法(Westem blot)检测IL-17R和Actl的蛋白表达；免疫荧光检测NF-kB核移位；ELISA检测上清的IL-17含量.结果:各组HepG2细胞培养上清液中均未检测到1L-17且亦未检测HepG2细胞有IL-17的mRNA表达；HBV重组腺病毒组的IL-17RmRNA和蛋白的表达明显低于相应浓度对照组(0.68±0.02 vs 0.89±0.03,0.33±0.06 vs0.81±0.01,0.12±0.01 vs 0.86±0.05,P＜0.05;蛋...

AIM： To investigate whether hepatitis B virus influences the expression of interleukin-17 （IL-17） receptor and the adaptor Act1 in HepG2 cells. METHODS： The mRNA and protein expression of IL-17 receptor and the adaptor Act1 was determined by real-time PCR and Western blot, respectively. NF-kappa B nuclear translocation was detected by immunofluorescence. The content of IL-17 in cell supernatants was measured by ELISA. RESULTS： IL-17 was not detected in the culture supernatants of HepG2 cells, and IL-17 mRNA expression was not detected in H...