期刊论文

Tang, S.-S.

中文

世界华人消化杂志

1009-3079

2006

14

15

1448-1452

10.3969/j.issn.1009-3079.2006.15.002

国家自然科学基金资助项目．No．30270684~~；

本校为第一且通讯机构

目的:研究Caveolin-1对胃癌细胞增殖、分化的影响,以探讨Caveolin-1作为基因治疗的候选基因的可能性．方法:运用基因重组技术,将人全长 Caveolin-1基因稳定转染胃癌细胞系MGC803．建立能稳定表达Caveolin-1的胃癌细胞系．同时建立空载体转染的MGC803细胞系作为空白对照,另用PD98059处理48 h作为阳性对照．以重组细胞系作为研究模型,通过免疫细胞化学及Western blot确认Caveolin-1蛋白在被转染细胞中的稳定表达．运用光学显微镜观察转染前后MGC803细胞形态的变化;另运用细胞计数检测了Caveolin-1对MGC803细胞生长的影响,流式细胞术分析了Caveolin-1对MGC803 细胞周期分布的影响．结果:Western bl...

AIM: To investigate the effect of Caveolin-1 on the proliferation and differentiation of gastric carcinoma cell line MGC803, and explore the probability that Caveolin-1 can be used for gene therapy. METHODS: Caveolin-1 gene and Pcl-neo control plasmid were transfected into human MGC803 cell line by lipofectin, respectively. The positive clones were selected by G418. We also stabled a positive control group which was treated with PD98059 for 48 hours. Then the expression of Caveolin-1 in each group was detected by Western blot. Cell morphology was observed under optical microscope. Cell populat...