期刊论文

Effect of Hydrogen Sulfide on Kidney Injury Induced by Urinary-Derived Sepsis

中文

天津医药

0253-9896

2014

8

769-773,850

10.3969/j.issn.0253-9896.2014.08.010

湖南省自然科学基金项目（13JJ9009）；

本校为第一机构

目的：探讨H2S对尿源性脓毒血症肾损伤的影响及其机制。方法将30只大白兔随机均分为Control组、Sham组、Sepsis组、NaHS 2.8μmol/kg组和NaHS 8.4μmol/kg组。建立上尿路急性梗阻并感染脓毒血症动物模型。于术前、术后24 h、48 h、72 h采血行外周血检测白细胞计数(WBC)、血肌酐（Cr）、尿素氮(BUN）。术后72 h光学显微镜及透射电子显微镜观察肾组织形态结构变化；应用免疫组织化学法检测肾组织肿瘤坏死因子（TNF）-α、白细胞介素（IL）-10和核因子（NF）-κB蛋白表达；去蛋白法测定血浆H2S浓度，亚甲基蓝分光光度计法测肾组织胱硫醚-γ-裂解酶(CSE)的活性。结果术后24 h、48 h、72 h，Sepsis组...

Objective To investigate the effect and mechanism of hydrogen sulfide (H2S) on kidney injury induced by urinary-derived sepsis. Methods Rabbits were randomly divided into Control group, Sham group, Sepsis group, NaHS 2.8μmol/kg group and NaHS 8.4μmol/kg group. Upper urinary tract obstruction and acute infection was induced to estab-lish Sepsis model. At 24 h before surgery, and 24 h, 48 h, 72 h after surgery, blood was taken to examine white blood cell count (WBC), creatinine (Cr) and blood urea nitrogen (BUN). At 72 h after surgery, morpholo...