期刊论文

Construction of a Cascaded Copper Ion Fluorescence Detection System Based onλExonuclease

中文

南华大学学报(自然科学版)

1673-0062

2022

36

3

69-76

10.19431/j.cnki.1673-0062.2022.03.010

国家自然科学基金项目（11475079）；

本校为第一机构

通过将一种有效的级联信号放大策略引入铜离子荧光生物传感器中,构建了一种用于超灵敏检测铜离子的荧光检测体系.这种级联型荧光检测体系结合了λ核酸外切酶辅助的靶标回收和催化发夹自组装的循环信号扩增.在铜离子的存在下,用于识别铜离子的功能核酸的底物链被特异性切割和释放.释放出的单链DNA片段(tDNA)与5′端被磷酸标记的双链DNA(TQ-DNA)杂交形成具有平末端的双链DNA.随后,该DNA分子被 λ 核酸外切酶识别并切割,释放出游离的tDNA和TDNA.随着λ核酸外切酶的水解,越来越多的tDNA被回收,同时放出越来越多的TDNA.此时,TDNA作为催化发夹自组装反应的触发链,启动了新一轮的靶标循环.在TDNA的辅助...

An efficiently cascaded signal amplification strategy was constructed and introduced into a fluorescent biosensor for ultrasensitive detection of copper ions(Cu2+).This cascaded fluorescence detection system combinesλexonuclease(λ-exo)-assisted target recovery and cyclic signal amplification of catalyzed hairpin self-assembly(CHA).In the presence of Cu2+,the substrate strand of the functional nucleic acids for the recognition of copper ions are specifically cleaved and released.Then,these released single-stranded DNA fragments(tDNAs)hybridize...