期刊论文

中文

南华大学学报(自然科学版)

1673-0062

2018

32

2

7-12

10.19431/j.cnki.1673-0062.20180611.002

国家自然科学基金项目（11475079）；

本校为第一机构

建立了一种利用二级散射法不经分离直接测定溶液中ATP的新方法.利用间苯二甲醛与吡咯反应制备一种新型双极双齿配体(isophthalaldehyde-tetrapyrrole,IP TP),该配体与铀酰配位形成双核铀酰配合物,双核铀酰配合物与ATP络合形成双核铀酰配合物-ATP,研究双核铀酰配合物-ATP体系的光谱特征,影响因素和最佳反应条件.在最佳条件下二级散射强度与ATP的浓度的线性范围是2.5～500 nmol/L,检出限为0.75 nmol/L,此方法已成功用于待测样品中ATP的测定.

This paper has established a new method for the direct determination of ATP in solution without separation by the second-order scattering（ SOS） method.A dipolar bidentate ligand of isophthalaldehyde-tetrapyrrole（ IPTP） was synthesized by the reaction of isophthalaldehyde and pyrrole. The ligand was mixed with uranyl to form binuclear uranyl complex（ BUIPTP）,then BUIPTP complexes reacted with ATP to form BUIPTP-ATP.The influence factors,the best reaction conditions,and the spectral characteristics of BUIPTPATP system were studied.Under the...