期刊论文

中文

中南医学科学杂志

2095-1116

2017

45

6

558-561+579

10.15972/j.cnki.43-1509/r.2017.06.006

13C824:湖南省教育厅科学研究项目 CX2015B415:湖南省研究生科研创新项目 No.2015-351:湖南省分子靶标新药研究协同创新中心资助项目 NO.2014-5:特殊病原体防控湖南省重点实验室项目

本校为第一机构

目的 构建日本血吸虫动力蛋白轻链DNA疫苗和小鼠干扰素真核表达质粒,研究其在He La细胞及动物体内的表达。方法 设计特异性引物,PCR扩增获取动力蛋白轻链（Sj DLC）与干扰素-γ（IFN-γ）基因,克隆于pc DNA3.1真核质粒,经双酶切及测序鉴定。将两种重组质粒分别转染至He La细胞,Western blot鉴定其表达。真核质粒经左腿股四头肌免疫小鼠,在末次免疫2周后,PCR法检测小鼠肌组织内Sj DLC和IFN-γ基因,免疫组织化学法检测基因的表达,MTT法检测T细胞增殖水平。结果 PCR和双酶切能检测到280 bp的Sj DLC基因及480 bp的IFN-γ基因;Western blot显示在12 k Da和19 k Da处有阳性反应条带,与Sj DLC和IFN-γ分...

Objective To construct the dynein light chain of Schistosoma japonicum DNA vaccine and IFN-γ recombi- nant plasmid of mice,so as to investigate their expression in HeLa cells and mouse. Methods A pair of primers designed by PRIMER5.0 software was synthesized;the gene fragments of SjDLC and mIFN-γ/were amplified by PCR, and inserted into eukaryotic expression plasmid pcDNA3.1, where the recombinant plasmids were identified with restrictive enzymes and sequenced.The recombinant vectors pcDNA3.1/SjDLC and pcDNA3.1/mIFN-γ/were transfected into H...