期刊论文

中文

中南医学科学杂志

2095-1116

2017

45

1

48-53

10.15972/j.cnki.43-1509/r.2017.01.011

2011GZK46:国家自然科学基金 2012TT2020:湖南省科技厅项目 231:湖南省大学生研究性学习与创新性实验资助项目

本校为第一机构

目的 运用生物信息学预测并分析鼻咽癌抑癌基因STGC3启动子,构建双荧光素酶报告基因表达载体. 方法 采用生物信息学软件预测STGC3基因5'端上游调控区5 000 bp序列进行启动子预测与分析,克隆最有可能的启动子,构建萤火虫荧光素酶双报告基因重组质粒,重组质粒经MluⅠ和BglⅡ限制性内切酶酶切及测序鉴定.结果 STGC3基因5'端上游-3 046 bp至-46 bp区域内有启动子活性,在-2 992 bp至-69 bp间启动子分值达0.8以上,其中-845 bp至-795 bp间分值最高,达到1.0.在-1 140 bp和-774 bp处检测到GC盒信号;在-441 bp处检测到CAAT盒信号.在-1 805 bp至-1 705 bp和-900 bp至-684 bp间各有一个CpG岛;在-2 348 bp和-...

Objective Using bioinformatics to predict and analyse the promoter of STGC3 gene,then construct dual luciferase reporter gene expression vector for 5＇ Upstream regulated elements of STGC3 gene. Methods The bioinformatics was used to predict and analyze the promoter region of STGC3 during gene 5＇upstream 5000 bp regulatory region,and then the most likely promoters were cloned.The dual-luciferase reporter gene plasmids were constructed,and these recombinant plasmids were identified by the MluⅠand BglⅡenzyme digestion and DNA sequencing. Resul...