期刊论文

中文

中南医学科学杂志

2095-1116

2017

45

2

127-131

10.15972/j.cnki.43-1509/r.2017.02.005

湖南省教育厅科学研究项目（16C1401）资助,湖南省教育厅优秀青年基金项目（13B098）资助；

本校为第一机构

目的 分析丝裂霉素（MMC）治疗对胃癌细胞mi RNA表达谱的影响,为MMC的胃癌治疗机制提供理论参考。方法 体外培养胃癌SGC-7901细胞,根据噻唑兰（MTT）法评估MMC的作用效果和作用浓度;利用mi RNA芯片技术评估MMC干预后SGC-7901的mi RNA表达谱改变,并通过q RT-PCR验证差异表达mi RNA。结果SGC-7901细胞生长抑制率与MMC作用浓度间存在正相关;MMC干预48 h后SGC-7901细胞有59个mi RNA出现表达改变,与正常胃黏膜上皮细胞GES-1相比,SGC-7901胃癌细胞的mi R-205＊和mi R-3924等11个mi RNAs表达上调,而mi R-498,mi R-18b,mi R-196b,mi R-1247等22个mi RNA表达下调;经MMC干预后,部分异常表达得到恢复。结论...

Objective To evaluate the mitomycin C （MMC） treatment on the miRNA expression profile in gastric cancer cells, and provide the theoretical evidences for MMC treatment on gastric cancer. Methods The effects and opti- mum concentration of MMC were evaluated in the cultured SGC-7901 cells by MMT. The miRNA microarray technique was used for the exploration of miRNA profile of SGC-7901 cells after MMC treatment and the different expression of candidate miRNA was further confirmed by qRT-PCR. Results The inhibition rate of SGC-7901 cells was negati...