期刊论文

The Effect of NSCs Transplantation on the Expression of MAP-2 and GFAP in Rabbits with Lesion of Superior Cervical Sympathetic Ganglion

中文

中南医学科学杂志

2095-1116

2016

44

4

400-403

10.15972/j.cnki.43-1509/r.2016.04.010

2013SCX17:湖南省研究生科研创新项目 2015KJ21:衡阳市科学技术发展计划

本校为第一机构

目的：研究神经干细胞(NSCs)移植对颈上交感神经节(SCSG)损毁兔胶质纤维酸性蛋白(GFAP)以及微管相关蛋白2(MAP2)表达的影响。方法32只新西兰兔中8只为正常组不予处理,24只通过持针钳持续压榨兔SCSG 10 s的方法构建SCSG损毁模型,均分成模型组、培养液组和干细胞组,造模后第7天分别给予等量的生理盐水、培养液及2×106个神经干细胞,移植后第1、7、10、14、21天分别记录各组兔损伤侧瞳孔直径变化,第21天取损伤侧SCSG进行HE染色,并进行免疫组化检测MAP￣2和GFAP的表达。结果瞳孔直径变化情况,第10天、14天、21天干细胞组瞳孔直径均大于模型组和培养液组,差异有显著性( P

Objective This study was to investigate the effects of Neural stem cells transplantation on the expression of MAP2 and GFAP for treatment in superior cervical sympathetic ganglion injury in rabbits. Methods 8 of the 32 New Zealand rabbits were recruited in normal group and no intervention was made,24 SCSG damage models were constructed by the method of continuous pressing of rabbit＇s SCSG for 10 seconds ,which were divided into model group, culture-medium group and stem cells group,the same amount of normal saline,culture media and 2 × 106 ne...