期刊论文

Effect of In vitro Passage with Selection Pressure on Fitness of Plasmid-free Chlamydia Muridarum Strain CMUT3

中文

中南医学科学杂志

2095-1116

2016

44

4

384-389

10.15972/j.cnki.43-1509/r.2016.04.007

国家自然科学基金（81072417）.

本校为第一机构

目的：探讨传代选择对鼠衣原体质粒缺失株CMUT3菌株培养特性的影响及其相应机制。方法采用“无辅助感染”和“辅助感染”交替方式体外传代培养CMUT3菌株,而后观察传代菌株和亲代感染细胞时的吸附效率以及对离心因素的依赖性,并利用下一代测序技术对传代菌株CMUT3G40和亲代CMUT3G0进行全基因组测序分析。结果传代菌株CMUT3G40在感染HeLa细胞时对离心因素依赖性降低,吸附试验表明CMUT3G40菌株对细胞的吸附能力增强；比较基因组学分析结果表明 CMUT3G40菌株与亲代 CMUT3G0在 tc0237基因存在差异, TC0237Q117E错义突变出现于CMUT3G40菌株基因组( CMUT3G40,99.7％；CMUT3G0,0％)。结论通过体外传代培...

Objective To unravel the effect of passage selection pressure on fitness of plasmid-free Chlamydia muri- darnm strain CMUT3 and uncover the correlation between genetic and phenotypic signatures. Methods CMUT3 organisms were in vitro passaged for 40 generations under the alternating unassisted/assisted-infection cycle conditions and the infectivity of initial and final generation organisms in cell culture was measured by comparing their attachment efficiency phenotype in the presence or absence of centrifugation.Next-generation sequencing techno...