期刊论文

FGF-2 Inhibits Cardiomyocytes Apoptosis Induced by Hypoxia/reoxygenation via Upregulating the Expression of Survivin in H9c2 cells

中文

中南医学科学杂志

2095-1116

2016

44

4

379-383,389

10.15972/j.cnki.43-1509/r.2016.04.006

81470435、81541005:国家自然科学基金 B2011￣041:湖南省卫生厅科学研究项目

本校为第一机构

目的：探讨成纤维细胞生长因子￣2( FGF￣2)拮抗心肌细胞凋亡的作用机制。方法以H9c2大鼠心肌细胞作为实验对象,用无血清培养基同步化培养24小时,心肌细胞随机分为正常组( Control)、缺氧/复氧( H/R)组和H/R＋FGF￣2组。采用CCK￣8比色法检测心肌细胞存活率,Hoechst染色和流式细胞术检测心肌细胞凋亡。采用Western blot检测生存素(Survivin)蛋白的表达。结果与正常组相比,H/R明显抑制心肌细胞活力,诱导心肌细胞凋亡(均P

Objective To investigate the mechanism of FGF-2＇ s fighting against H9c2 cardiomyocytes apoptosis in- duced by Hypoxia/reoxygenation（H/R）. Methods H9c2 cardiomyocytes was employed as the experimental object,cells were incubated by free serum for 24 h for synchronous cell cycle, then, H9c2 cardiomyocytes were randomly divided into 3 groups：Normal group（Control） ,H/R group, H/R＋FGF-2 group.The viability of cardiomyocytes was measured by CCK-8. The apoptosis rate of cardiomyocytes was detected by Hoechst and Armexin-V/PI assays with flow cy...