期刊论文

D-ribose Induced Rapid Non-enzymatic Glycation of Human Myoglobin

中文

中南医学科学杂志

2095-1116

2016

5

499-503

10.15972/j.cnki.43-1509/r.2016.05.005

湖南省教育厅资助科研项目（13C817）； 湖南省卫生计生委科研计划课题项目（C2016041）；

本校为第一机构

目的探讨D-核糖与D-葡萄糖对人肌红蛋白（HMb）分子构象的影响,并研究反应后晚期糖基化终末产物（AGEs）的形成情况,以期为糖尿病及其并发症的防治提供新的研究线索。方法表达、纯化获得HMb,在体外分别与D-核糖及D-葡萄糖共孵育,采用紫外可见光谱（UV-vis）监测反应后HMb的结构变化,利用荧光光谱法监测反应后AGEs的生成情况。通过SDS-PAGE观察HMb与D-核糖及D-葡萄糖反应后分子量的变化情况。结果 UV-vis显示D-核糖可在短时间内导致HMb血红素活性中心构象发生改变,且呈时间依赖性;同时随着核糖糖化时间的延长,具自发荧光的AGEs生成也逐渐增多。SDS-PAGE结果显示D-核糖能很快地与HMb发生反应,形成...

Objective To investigate the effect of D￣ribose and D￣glucose on molecular conformation of human myo￣globin( HMb) ,determine the formation of advanced glycation end products ( AGEs) through in vitro glycation,and provide new clues for the prevention and treatment of diabetes mellitus and its complications. Method HMb was expressed in BL21(DE3),purified,and incubated with D￣ribose or D￣glucose.Structural changes of HMb were studied by using UV￣Vis spectroscopy.The formation of AGEs was monitored by Fluorescence spectrum.The molecular weigh...