期刊论文

Up-regulation of RORα Induced by Diallyl Disulfide Inhibiting Proliferaion, Migration and Invasion of Human Gastric Cancer MGCS03 Cells

中文

中南医学科学杂志

2095-1116

2016

44

6

604-609

10.15972/j.cnki.43-1509/r.2016.06.002

81374013，81102854:国家自然科学基金 B2015-182:湖南省卫生计生委科研项目

本校为第一机构

目的研究二烯丙基二硫( DADS)是否通过上调维甲酸相关孤核受体α( RORα)表达抑制人胃癌MGC803细胞增殖、细胞周期与迁移侵袭。方法实验组分为6组,对照组,空载体组, RORα干扰组( miR-RORα),DADS组,空载体联合DADS组,miR-RORα联合DADS组。 Western blot检测RORα、MMP-9与TIMP3蛋白表达。 MTT、流式细胞术、迁移和侵袭实验分别检测细胞增殖、细胞周期与迁移侵袭的改变。结果 DADS处理细胞24 h后,与对照组和空载体组比较,DADS上调RORα和TIMP3、下调MMP-9蛋白表达。干扰组较对照组与空载体组RORα表达明显下调。与DADS处理组比较,干扰RORα表达上调MMP-9、下调TIMP3蛋白表达。 MTT实验显示,DAD...

Objective To investigate whether diallyl disulfide （DADS） inhibits human gastric cancer MGC803 cell proliferation, migration and invasion by up-regulating the expression of RORα. Methods The experimental group was divided into six groups, control group, vector group, DADS group, vector＋DADS group, and miR-RORα＋DADS group. RORα, miR-RORα, MMP-9 and TIMP3 protein levels were detected by using Western blot. MTF and flow cytometry were used for cell proliferation and cell cycle assays.Wound healing and transweU assays were performed to exam...