期刊论文

Mycoplasma Genitalium-derived Lipid-associated Membrane Proteins Induces Placental Trophoblast Cells Expression of HO-1 via ROS/Nrf2

中文

中南医学科学杂志

2095-1116

2015

4

364-368

10.15972/j.cnki.43-1509/r.2015.04.002

国家自然科学基金（31000091,81072418）； 特殊病原体防控湖南省重点实验室资助项目（湘科计字[2014]5号,湘教通〔2012〕312号）；

本校为第一机构

目的：探讨生殖支原体脂质相关膜蛋白(LAMPs)诱导胎盘滋养层细胞表达血红素氧合酶-1(HO-1)的分子机制。方法用0.5~5μg/mL生殖支原体LAMPs处理体外培养的胎盘滋养层细胞4~12 h,采用实时定量PCR和Western blot法分别检测HO-1 mRNA和蛋白的表达以及核因子相关因子-2(Nrf2)的核转位；比色法观察HO-1的酶活性；2ˊ,7ˊ-二氯二氢荧光黄二乙酸酯( H2 DCFDA)检测活性氧产生。分别采用酪氨酸激酶c-Src抑制剂PP1、活性氧抑制剂N-乙酰半胱氨酸( NAC)和Nrf2 siRNA干预,观察HO-1的表达情况。结果生殖支原体LAMPs能诱导滋养层细胞HO-1 mRNA和蛋白的表达,上调其酶活性。同时,LAMPs也能诱导其产生活性氧,并促使...

Objective To investigate the mechanism of the expression of heme oxygenase-1（ HO-1） in response to Mycoplasma genitalium-derived lipid-associated membrane proteins（ LAMPs） in placental trophoblast cells. Methods Placental trophoblast cells were cultured in vitro and stimulated by 0. 5 ~ 5 μg / m L of LAMPs for 4 ~ 12 h,expression of HO-1 mRNA and protein,and nuclear translocation of nuclear factor erythroid-2 related factor 2（ Nrf2） were detected by realtime PCR and Western blot,respectively. The enzyme activity of HO-1 was detected by c...