期刊论文

Synthesis and Electrochemical Property of Ferrocene-peptide Fc-Lys-Leu-Val-OCH3

中文

南华大学学报(自然科学版)

1673-0062

2015

3

92-95+98

10.19431/j.cnki.1673-0062.2015.03.022

本校为第一机构

以二茂铁、亮氨酸、缬氨酸和赖氨酸为原料,苯并***-1-四甲基六氟磷酸酯( HBTU)及1-羟基苯并***( HOBt)为缩合剂,采用液相合成法,合成了Fc-Lys-Leu-Val-OCH3(简称Fc-KLV,其中Fc：ferrocenoyl,二茂铁).用柱层析分离纯化得到目标化合物,并对目标化合物进行紫外和核磁表征以确证其结构. Fc-KLV的循环伏安扫描结果表明,在0.4~0.8 V范围内出现了一对可逆的氧化还原峰,氧化峰和还原峰电位分别为624 mV和546 mV,峰电位之差ΔEp为78 mV,峰电流密度之比Ipa/Ipc =1.12,扩散系数D=1.782×10-6 cm2/s,表明相应的电化学过程主要受扩散控制.

Electrochemical peptide Fc-Lys-Leu-Val-OCH3 （ Fc-KLV, Fc ： ferrocenoyl） was synthesized from ferrocene, Leu, Val and Lys by solution phase peptide synthesis method u- sing O- （ benzotriazol -yl ） -N, N, N＇, N＇-tetramethyluronium （ HBTU ） / 1 -hydroxybenzotrizole （HOBt） as coupling reagents. The crude Fc-peptide was purified by column ehromatogra- phy, and then was characterized by UV and NMR. The formal redox potential, Epa and Epc at 624 mV and 546 mV, respectively, and Ipa/Ipc at 1.12 were characteristic for the Fe-peptide undergoi...