期刊论文

Proteomics Analysis of Differential Expression Proteins in Gastric Carcinoma

中文

中南医学科学杂志

2095-1116

2015

1

14-20

10.15972/j.cnki.43-1509/r.2015.01.004

12JJ3102:湖南省自然科学基金 B2012-046:湖南省卫生厅科学研究项目 2013HKJ19:衡阳市科技局项目 13K082:湖南省高等学校创新平台开放基金 2012105555017:国家级大学生创新创业训练计划项目

本校为第一机构

目的：筛选与鉴定胃癌差异表达蛋白质分子。方法采用定量蛋白组学方法，通过同位素iTRAQ标记胃癌与正常胃黏膜组织总蛋白，然后用强阳离子交换/反相液相色谱( SCX/nanoHPLC)进行电喷雾串联质谱分析( ESI-MS/MS)，获得两组样本的多肽及相对丰度信息，通过数据库搜索与比对，鉴定差异表达蛋白质。结果共鉴定出319个蛋白质，其中表达差异在2倍以上的88个，在胃癌中呈高表达的12个、呈低表达的76个。按蛋白质功能分为七大类：细胞骨架蛋白、分子伴侣、信号传导、生物代谢、凋亡，蛋白质合成与代谢类及其他蛋白质。结论成功筛选了胃癌差异表达蛋白质分子，这些蛋白质的表达改变，可能参与了胃癌的发生...

Objective To identify the differential expression proteins between gastric adenocarcinoma cells ( GAC) and normal gastric adenoepithelial cells ( NGAC) ,and to look for early diagnosis of Gastric carcinoma ( GC ) specific pro-teins. Methods The total proteins were extracted from the tissues. The total proteins labeled with different iTRAQ stable isotope were segregated with Nano-RPLC-MS/MS,to obtain relative abundance of peptides information. The differential ex-pression proteins were identified through SWISSPORT database. Results Through Quantitative proteomic analysis of GAC and NGAC,319 pro...